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Protein

ATPase inhibitor, mitochondrial

Gene

Atp5if1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase inhibitor, mitochondrialCurated
Alternative name(s):
ATP synthase F1 subunit epsilonImported
Inhibitor of F(1)F(o)-ATPase
Short name:
IF(1)
Short name:
IF1
Gene namesi
Name:Atp5if1Imported
Synonyms:Atpi, Atpif1, If1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2181 Atp5if1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionAdd BLAST25
ChainiPRO_000000255126 – 107ATPase inhibitor, mitochondrialAdd BLAST82

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineBy similarity1
Modified residuei103N6-succinyllysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03344
PRIDEiQ03344

PTM databases

iPTMnetiQ03344
PhosphoSitePlusiQ03344

Expressioni

Gene expression databases

BgeeiENSRNOG00000013300 Expressed in 9 organ(s), highest expression level in colon
GenevisibleiQ03344 RN

Interactioni

Subunit structurei

Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ03344
STRINGi10116.ENSRNOP00000018038

Structurei

3D structure databases

ProteinModelPortaliQ03344
SMRiQ03344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 52N-terminal inhibitory regionBy similarityAdd BLAST27
Regioni74 – 106Antiparallel alpha-helical coiled coil regionBy similarityAdd BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili60 – 107Sequence analysisAdd BLAST48

Domaini

Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 74-106, leaving each N-terminal inhibitory region (residues 26-52) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 26-52) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the ATPase inhibitor family.Curated

Keywords - Domaini

Coiled coil, Transit peptide

Phylogenomic databases

eggNOGiENOG410J2TJ Eukaryota
ENOG41127Z5 LUCA
GeneTreeiENSGT00390000006264
HOGENOMiHOG000247022
HOVERGENiHBG061381
InParanoidiQ03344
KOiK22255
OMAiAEEEMYF
OrthoDBiEOG091G1B33
PhylomeDBiQ03344
TreeFamiTF320659

Family and domain databases

InterProiView protein in InterPro
IPR007648 ATPase_inhibitor_mt
PfamiView protein in Pfam
PF04568 IATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03344-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGSALAVRA RLGVWGMRVL QTRGFGSDSS ESMDSGAGSI REAGGAFGKR
60 70 80 90 100
EKAEEDRYFR EKTREQLAAL KKHHEDEIDH HSKEIERLQK QIERHKKKIK

YLKNSEH
Length:107
Mass (Da):12,248
Last modified:February 1, 1996 - v2
Checksum:iC2BE7DF21AC86AF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 11MAGSALAVRAR → MTKSCRIEAST in AAA41360 (PubMed:8442667).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07806 mRNA Translation: AAA41360.1
D13122 mRNA Translation: BAA02424.1
U12250 Genomic DNA Translation: AAA85094.1
BC126065 mRNA Translation: AAI26066.1
PIRiJS0738
RefSeqiNP_037047.2, NM_012915.2
UniGeneiRn.1658

Genome annotation databases

EnsembliENSRNOT00000018038; ENSRNOP00000018038; ENSRNOG00000013300
GeneIDi25392
KEGGirno:25392
UCSCiRGD:2181 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07806 mRNA Translation: AAA41360.1
D13122 mRNA Translation: BAA02424.1
U12250 Genomic DNA Translation: AAA85094.1
BC126065 mRNA Translation: AAI26066.1
PIRiJS0738
RefSeqiNP_037047.2, NM_012915.2
UniGeneiRn.1658

3D structure databases

ProteinModelPortaliQ03344
SMRiQ03344
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ03344
STRINGi10116.ENSRNOP00000018038

PTM databases

iPTMnetiQ03344
PhosphoSitePlusiQ03344

Proteomic databases

PaxDbiQ03344
PRIDEiQ03344

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018038; ENSRNOP00000018038; ENSRNOG00000013300
GeneIDi25392
KEGGirno:25392
UCSCiRGD:2181 rat

Organism-specific databases

CTDi93974
RGDi2181 Atp5if1

Phylogenomic databases

eggNOGiENOG410J2TJ Eukaryota
ENOG41127Z5 LUCA
GeneTreeiENSGT00390000006264
HOGENOMiHOG000247022
HOVERGENiHBG061381
InParanoidiQ03344
KOiK22255
OMAiAEEEMYF
OrthoDBiEOG091G1B33
PhylomeDBiQ03344
TreeFamiTF320659

Miscellaneous databases

ChiTaRSiAtpif1 rat
PROiPR:Q03344

Gene expression databases

BgeeiENSRNOG00000013300 Expressed in 9 organ(s), highest expression level in colon
GenevisibleiQ03344 RN

Family and domain databases

InterProiView protein in InterPro
IPR007648 ATPase_inhibitor_mt
PfamiView protein in Pfam
PF04568 IATP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATIF1_RAT
AccessioniPrimary (citable) accession number: Q03344
Secondary accession number(s): A0JMZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: September 12, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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