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Protein

Cholinesterase

Gene

Bche

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.2 Publications

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei227Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei354Charge relay systemBy similarity1
Active sitei467Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-112311 Neurotransmitter clearance
R-MMU-1483191 Synthesis of PC
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
SABIO-RKiQ03311

Protein family/group databases

ESTHERimouse-BCHE BCHE
MEROPSiS09.980

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:Bche
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:894278 Bche

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to the presence of other cholinesterases. Hypersensitive to acetylcholinesterase inhibitors, such as huperzine and donepezil. Treatment with the acetylcholinesterase inhibitor donepezil causes convulsions and death within 3 hours of dosing.2 Publications

Chemistry databases

ChEMBLiCHEMBL2528

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000000861430 – 603CholinesteraseAdd BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi94 ↔ 121By similarity
Glycosylationi135N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei227PhosphoserineBy similarity1
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi281 ↔ 292By similarity
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi429 ↔ 548By similarity
Glycosylationi484N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi510N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi600InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ03311
PaxDbiQ03311
PeptideAtlasiQ03311
PRIDEiQ03311

PTM databases

iPTMnetiQ03311
PhosphoSitePlusiQ03311

Expressioni

Tissue specificityi

Present in most cells except erythrocytes.

Gene expression databases

BgeeiENSMUSG00000027792 Expressed in 195 organ(s), highest expression level in jejunum
CleanExiMM_BCHE
ExpressionAtlasiQ03311 baseline and differential
GenevisibleiQ03311 MM

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029367

Chemistry databases

BindingDBiQ03311

Structurei

3D structure databases

ProteinModelPortaliQ03311
SMRiQ03311
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 146Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00760000118946
HOGENOMiHOG000091866
HOVERGENiHBG008839
InParanoidiQ03311
KOiK01050
OMAiFPGSEMW
OrthoDBiEOG091G0I4G
TreeFamiTF315470

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR014788 AChE_tetra
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
IPR000997 Cholinesterase
PfamiView protein in Pfam
PF08674 AChE_tetra, 1 hit
PF00135 COesterase, 1 hit
PRINTSiPR00878 CHOLNESTRASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD415333 AChE_tetra, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q03311-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQTQHTKVTQ THFLLWILLL CMPFGKSHTE EDFIITTKTG RVRGLSMPVL
60 70 80 90 100
GGTVTAFLGI PYAQPPLGSL RFKKPQPLNK WPDIHNATQY ANSCYQNIDQ
110 120 130 140 150
AFPGFQGSEM WNPNTNLSED CLYLNVWIPV PKPKNATVMV WIYGGGFQTG
160 170 180 190 200
TSSLPVYDGK FLARVERVIV VSMNYRVGAL GFLAFPGNPD APGNMGLFDQ
210 220 230 240 250
QLALQWVQRN IAAFGGNPKS ITIFGESAGA ASVSLHLLCP QSYPLFTRAI
260 270 280 290 300
LESGSSNAPW AVKHPEEARN RTLTLAKFTG CSKENEMEMI KCLRSKDPQE
310 320 330 340 350
ILRNERFVLP SDSILSINFG PTVDGDFLTD MPHTLLQLGK VKKAQILVGV
360 370 380 390 400
NKDEGTAFLV YGAPGFSKDN DSLITRKEFQ EGLNMYFPGV SRLGKEAVLF
410 420 430 440 450
YYVDWLGEQS PEVYRDALDD VIGDYNIICP ALEFTKKFAE LENNAFFYFF
460 470 480 490 500
EHRSSKLPWP EWMGVMHGYE IEFVFGLPLG RRVNYTRAEE IFSRSIMKTW
510 520 530 540 550
ANFAKYGHPN GTQGNSTMWP VFTSTEQKYL TLNTEKSKIY SKLRAPQCQF
560 570 580 590 600
WRLFFPKVLE MTGDIDETEQ EWKAGFHRWS NYMMDWQNQF NDYTSKKESC

TAL
Length:603
Mass (Da):68,462
Last modified:July 27, 2011 - v2
Checksum:i2CB79C46797B3713
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YVZ5A0A0A6YVZ5_MOUSE
Cholinesterase
Bche
174Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129P → R in AAA37328 (PubMed:2400605).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99492 mRNA Translation: AAA37328.1
AK050337 mRNA Translation: BAC34196.1
CH466547 Genomic DNA Translation: EDL15482.1
CCDSiCCDS17411.1
PIRiS70849
RefSeqiNP_033868.3, NM_009738.3
UniGeneiMm.250719

Genome annotation databases

EnsembliENSMUST00000029367; ENSMUSP00000029367; ENSMUSG00000027792
GeneIDi12038
KEGGimmu:12038
UCSCiuc008pmv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99492 mRNA Translation: AAA37328.1
AK050337 mRNA Translation: BAC34196.1
CH466547 Genomic DNA Translation: EDL15482.1
CCDSiCCDS17411.1
PIRiS70849
RefSeqiNP_033868.3, NM_009738.3
UniGeneiMm.250719

3D structure databases

ProteinModelPortaliQ03311
SMRiQ03311
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029367

Chemistry databases

BindingDBiQ03311
ChEMBLiCHEMBL2528

Protein family/group databases

ESTHERimouse-BCHE BCHE
MEROPSiS09.980

PTM databases

iPTMnetiQ03311
PhosphoSitePlusiQ03311

Proteomic databases

MaxQBiQ03311
PaxDbiQ03311
PeptideAtlasiQ03311
PRIDEiQ03311

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029367; ENSMUSP00000029367; ENSMUSG00000027792
GeneIDi12038
KEGGimmu:12038
UCSCiuc008pmv.1 mouse

Organism-specific databases

CTDi590
MGIiMGI:894278 Bche

Phylogenomic databases

eggNOGiKOG4389 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00760000118946
HOGENOMiHOG000091866
HOVERGENiHBG008839
InParanoidiQ03311
KOiK01050
OMAiFPGSEMW
OrthoDBiEOG091G0I4G
TreeFamiTF315470

Enzyme and pathway databases

ReactomeiR-MMU-112311 Neurotransmitter clearance
R-MMU-1483191 Synthesis of PC
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
SABIO-RKiQ03311

Miscellaneous databases

PROiPR:Q03311
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027792 Expressed in 195 organ(s), highest expression level in jejunum
CleanExiMM_BCHE
ExpressionAtlasiQ03311 baseline and differential
GenevisibleiQ03311 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR014788 AChE_tetra
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
IPR000997 Cholinesterase
PfamiView protein in Pfam
PF08674 AChE_tetra, 1 hit
PF00135 COesterase, 1 hit
PRINTSiPR00878 CHOLNESTRASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD415333 AChE_tetra, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHLE_MOUSE
AccessioniPrimary (citable) accession number: Q03311
Secondary accession number(s): Q543J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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