UniProtKB - Q03250 (RBG7_ARATH)
Protein
Glycine-rich RNA-binding protein 7
Gene
RBG7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Plays a role in RNA transcription or processing during stress. Binds RNAs and DNAs sequence with a preference to single-stranded nucleic acids. Displays strong affinity to poly(U) and poly(G) sequence. Involved in mRNA alternative splicing of numerous targets by modulating splice site selection. Negatively regulates the circadian oscillations of its own transcript as well as RBG8 transcript. Forms an interlocked post-transcriptional negative feedback loop with the RBG8 autoregulatory circuit. Both proteins negatively autoregulate and reciprocally crossregulate by binding to their pre-mRNAs and promoting unproductive splicing coupled to degradation via the NMD pathway. Involved in the regulation of abscisic acid and stress responses. Affects the growth and stress tolerance under high salt and dehydration stress conditions, and also confers freezing tolerance, particularly via the regulation of stomatal opening and closing in the guard cells. Exhibits RNA chaperone activity during the cold adaptation process. Involved in the export of mRNAs from the nucleus to the cytoplasm under cold stress conditions. Target of the Pseudomonas syringae type III effector HopU1, which could probably be involved in plant innate immunity. Component of the flowering autonomous pathway which promotes floral transition, at least partly by down-regulating FLC.15 Publications
Miscellaneous
Plants overexpressing RBG7 display retarded germination and affected seedling growth under salt and dehydration stress conditions, confer freezing tolerance and also possess enhanced resistance to P.syringae.
GO - Molecular functioni
- double-stranded DNA binding Source: TAIR
- mRNA binding Source: TAIR
- RNA binding Source: UniProtKB
- single-stranded DNA binding Source: TAIR
GO - Biological processi
- alternative mRNA splicing, via spliceosome Source: TAIR
- circadian rhythm Source: TAIR
- DNA duplex unwinding Source: TAIR
- innate immune response Source: TAIR
- mRNA export from nucleus Source: TAIR
- regulation of stomatal movement Source: TAIR
- response to cadmium ion Source: TAIR
- response to cold Source: UniProtKB
- response to osmotic stress Source: TAIR
- response to salt stress Source: UniProtKB
- response to water deprivation Source: UniProtKB
- response to zinc ion Source: TAIR
- RNA secondary structure unwinding Source: TAIR
- vegetative to reproductive phase transition of meristem Source: TAIR
Keywordsi
Molecular function | Chaperone, RNA-binding |
Biological process | Immunity, Innate immunity, mRNA processing, mRNA splicing, Plant defense |
Names & Taxonomyi
Protein namesi | Recommended name: Glycine-rich RNA-binding protein 7Short name: AtGR-RBP7 Alternative name(s): AtRBG7 Glycine-rich protein 7 Short name: AtGRP7 Protein COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2 Short name: Protein CCR2 |
Gene namesi | Name:RBG7 Synonyms:CCR2, GR-RBP7, GRP7 Ordered Locus Names:At2g21660 ORF Names:F2G1.7 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G21660 |
TAIRi | locus:2049359, AT2G21660 |
Subcellular locationi
Chloroplast
- chloroplast Source: TAIR
Cytosol
- cytosol Source: TAIR
Nucleus
- nucleus Source: UniProtKB
Peroxisome
- peroxisome Source: TAIR
Vacuole
- vacuole Source: TAIR
Other locations
- cytoplasm Source: UniProtKB
- plasmodesma Source: TAIR
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Hypersensitive responses to ABA in both seed germination and root growth. Late-flowering. Increased germination rate and seedling growth under salt and dehydration stress. Impaired mRNA export under cold stress conditions. Defective in PAMP-triggered immunity (PTI) responses and high susceptibility to P.syringae and P.carotovorum SCC1 (Pec).6 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 47 | R → K: Abolishes ADP-ribosylation by HopU1. 1 Publication | 1 | |
Mutagenesisi | 49 | R → K: Abolishes ADP-ribosylation by HopU1. Enable to complement the rbg7 mutant. 4 Publications | 1 | |
Mutagenesisi | 49 | R → Q: Impairs RNA-binding and consequently impairs the regulation of its pre-mRNA and its downstream pre-mRNA target RBG8. Affects the alternative splicing of numerous targets. 4 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000081599 | 2 – 176 | Glycine-rich RNA-binding protein 7Add BLAST | 175 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 49 | ADP-ribosylarginine; by HopU11 Publication | 1 | |
Modified residuei | 105 | PhosphoserineCombined sources | 1 | |
Modified residuei | 117 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
ADP-ribosylated by the Pseudomonas syringae type III effector HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.1 Publication
Keywords - PTMi
Acetylation, ADP-ribosylation, PhosphoproteinProteomic databases
PaxDbi | Q03250 |
PRIDEi | Q03250 |
ProMEXi | Q03250 |
ProteomicsDBi | 225967 [Q03250-1] |
PTM databases
iPTMneti | Q03250 |
MetOSitei | Q03250 |
SwissPalmi | Q03250 |
Expressioni
Tissue specificityi
Ubiquitous with strong expression in guard cell.2 Publications
Inductioni
Up-regulated by cold stress and down-regulated by dehydration stress, salt stress, abscisic acid (ABA) and mannitol. Circadian regulation. Induced by hydrogen peroxide (at the protein level). Up-regulated under P.carotovorum SCC1 (Pec) infection.8 Publications
Gene expression databases
ExpressionAtlasi | Q03250, baseline and differential |
Genevisiblei | Q03250, AT |
Interactioni
Subunit structurei
Interacts with TRN1.
Interacts with the Pseudomonas syringae type III effector HopU1.
2 PublicationsBinary interactionsi
Hide detailsQ03250
With | #Exp. | IntAct |
---|---|---|
EFR [C0LGT6] | 4 | EBI-1393626,EBI-8801168 |
FLS2 [Q9FL28] | 4 | EBI-1393626,EBI-1799448 |
hopU1 [Q88A91] from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000). | 2 | EBI-1393626,EBI-8802399 |
Protein-protein interaction databases
BioGRIDi | 2058, 8 interactors |
IntActi | Q03250, 41 interactors |
MINTi | Q03250 |
STRINGi | 3702.AT2G21660.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 86 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 41 | Required for RNA chaperone activityAdd BLAST | 40 | |
Regioni | 97 – 148 | Nuclear targeting sequence (M9)Add BLAST | 52 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 88 – 175 | Gly-richAdd BLAST | 88 |
Domaini
N-terminal part of the protein is one of the crucial determinant to confer RNA chaperone activity during cold adaptation process.1 Publication
Sequence similaritiesi
Belongs to the GR-RBP family.Curated
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
HOGENOMi | CLU_012062_28_1_1 |
InParanoidi | Q03250 |
OMAi | DAMTAMN |
OrthoDBi | 1579773at2759 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q03250-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASGDVEYRC FVGGLAWATD DRALETAFAQ YGDVIDSKII NDRETGRSRG
60 70 80 90 100
FGFVTFKDEK AMKDAIEGMN GQDLDGRSIT VNEAQSRGSG GGGGHRGGGG
110 120 130 140 150
GGYRSGGGGG YSGGGGSYGG GGGRREGGGG YSGGGGGYSS RGGGGGSYGG
160 170
GRREGGGGYG GGEGGGYGGS GGGGGW
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4IHK9 | F4IHK9_ARATH | Cold, circadian rhythm, and rna bin... | GRP7 ''cold, and rna binding 2, and rna binding 2'', ATGPR7, ATGRP7 | 159 | Annotation score: |
Sequence cautioni
The sequence L04172 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 133 | Missing in AAM62447 (Ref. 7) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045855 | 39 – 48 | IINDRETGRS → VCYTPRSDSE in isoform 2. 3 Publications | 10 | |
Alternative sequenceiVSP_045856 | 49 – 176 | Missing in isoform 2. 3 PublicationsAdd BLAST | 128 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z14987 mRNA Translation: CAA78711.1 L00648 mRNA Translation: AAA32853.1 L04172 mRNA No translation available. AC007119 Genomic DNA Translation: AAD23639.1 CP002685 Genomic DNA Translation: AEC07209.1 AF428381 mRNA Translation: AAL16149.1 AY054284 mRNA Translation: AAL06943.1 AY042826 mRNA Translation: AAK68766.1 Sequence problems. AY072523 mRNA Translation: AAL66938.1 Sequence problems. AK318968 mRNA Translation: BAH57083.1 Sequence problems. AY085214 mRNA Translation: AAM62447.1 |
PIRi | S30147 |
RefSeqi | NP_179760.1, NM_127738.5 [Q03250-1] |
Genome annotation databases
EnsemblPlantsi | AT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1] |
GeneIDi | 816705 |
Gramenei | AT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1] |
KEGGi | ath:AT2G21660 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z14987 mRNA Translation: CAA78711.1 L00648 mRNA Translation: AAA32853.1 L04172 mRNA No translation available. AC007119 Genomic DNA Translation: AAD23639.1 CP002685 Genomic DNA Translation: AEC07209.1 AF428381 mRNA Translation: AAL16149.1 AY054284 mRNA Translation: AAL06943.1 AY042826 mRNA Translation: AAK68766.1 Sequence problems. AY072523 mRNA Translation: AAL66938.1 Sequence problems. AK318968 mRNA Translation: BAH57083.1 Sequence problems. AY085214 mRNA Translation: AAM62447.1 |
PIRi | S30147 |
RefSeqi | NP_179760.1, NM_127738.5 [Q03250-1] |
3D structure databases
SMRi | Q03250 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2058, 8 interactors |
IntActi | Q03250, 41 interactors |
MINTi | Q03250 |
STRINGi | 3702.AT2G21660.1 |
PTM databases
iPTMneti | Q03250 |
MetOSitei | Q03250 |
SwissPalmi | Q03250 |
Proteomic databases
PaxDbi | Q03250 |
PRIDEi | Q03250 |
ProMEXi | Q03250 |
ProteomicsDBi | 225967 [Q03250-1] |
Genome annotation databases
EnsemblPlantsi | AT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1] |
GeneIDi | 816705 |
Gramenei | AT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1] |
KEGGi | ath:AT2G21660 |
Organism-specific databases
Araporti | AT2G21660 |
TAIRi | locus:2049359, AT2G21660 |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
HOGENOMi | CLU_012062_28_1_1 |
InParanoidi | Q03250 |
OMAi | DAMTAMN |
OrthoDBi | 1579773at2759 |
Miscellaneous databases
PROi | PR:Q03250 |
Gene expression databases
ExpressionAtlasi | Q03250, baseline and differential |
Genevisiblei | Q03250, AT |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBG7_ARATH | |
Accessioni | Q03250Primary (citable) accession number: Q03250 Secondary accession number(s): C0Z304, Q8LEV4, Q94B62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | October 7, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families