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Protein

Beta-ureidopropionase

Gene

Upb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts N-carbamoyl-beta-aminoisobutyrate and N-carbamoyl-beta-alanine (3-ureidopropanoate) to, respectively, beta-aminoisobutyrate and beta-alanine, ammonia and carbon dioxide.1 Publication

Catalytic activityi

3-ureidopropanoate + H2O = beta-alanine + CO2 + NH3.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Activity regulationi

Allosteric enzyme with positive cooperativity toward the substrate N-carbamoyl-beta-alanine.

Pathwayi: beta-alanine biosynthesis

This protein is involved in the pathway beta-alanine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway beta-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97ZincSequence analysis1
Active sitei119Proton acceptorPROSITE-ProRule annotation1
Metal bindingi158ZincSequence analysis1
Active sitei196Proton donorPROSITE-ProRule annotation1
Active sitei233NucleophilePROSITE-ProRule annotation1
Metal bindingi280ZincSequence analysis1
Metal bindingi293ZincSequence analysis1
Metal bindingi307ZincSequence analysis1

GO - Molecular functioni

  • beta-ureidopropionase activity Source: RGD
  • zinc ion binding Source: RGD

GO - Biological processi

  • beta-alanine biosynthetic process Source: RGD
  • beta-alanine biosynthetic process via 3-ureidopropionate Source: GO_Central
  • beta-alanine metabolic process Source: RGD
  • in utero embryonic development Source: RGD
  • liver development Source: RGD

Keywordsi

Molecular functionAllosteric enzyme, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15401
SABIO-RKiQ03248
UniPathwayi
UPA00131

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-ureidopropionase (EC:3.5.1.61 Publication)
Alternative name(s):
Beta-alanine synthase
N-carbamoyl-beta-alanine amidohydrolase
Gene namesi
Name:Upb1
Synonyms:Bup1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620091 Upb1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040541 – 393Beta-ureidopropionaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei378PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ03248

PTM databases

iPTMnetiQ03248
PhosphoSitePlusiQ03248

Interactioni

Subunit structurei

In the absence of ligands, the enzyme exists as a stable homohexamer, although this is not the most active form of the enzyme. This native hexamer dissociates to an inactive trimer in response to the product, beta-alanine, or associates to the more active homododecamer in response to the substrate.

Structurei

3D structure databases

ProteinModelPortaliQ03248
SMRiQ03248
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 344CN hydrolasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG018848
InParanoidiQ03248
KOiK01431
PhylomeDBiQ03248

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q03248-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGPEWQSLE QCLEKHLPPD DLSQVKRILY GKQTRNLDLP RKALEAASER
60 70 80 90 100
NFELKGYAFG AAKEQQRCPQ IVRVGLVQNR IPLPTSAPVA EQVSALHKRI
110 120 130 140 150
EEIAEVAAMC GVNIICFQEA WNMPFAFCTR EKLPWTEFAE SAEDGLTTRF
160 170 180 190 200
CQKLAKKHNM VVISPILERD RDHGGVLWNT AVVISNSGLV MGKTRKNHIP
210 220 230 240 250
RVGDFNESTY YMEGNLGHPV FQTQFGRIAV NICYGRHHPL NWLMYSVNGA
260 270 280 290 300
EIIFNPSATI GELSESMWPI EARNAAIANH CFTCALNRVG QEHYPNEFTS
310 320 330 340 350
GDGKKAHHDL GYFYGSSYVA APDGSRTPGL SRNQDGLLVT ELNLNLCQQI
360 370 380 390
NDFWTFKMTG RLEMYARELA EAVKPNYSPN IVKEDLVLAP SSG
Length:393
Mass (Da):44,042
Last modified:October 1, 1993 - v1
Checksum:i723E5AF5B01E3AAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97662 mRNA Translation: AAA40804.1
BC078767 mRNA Translation: AAH78767.1
PIRiA46624 S27881
RefSeqiNP_446297.1, NM_053845.1
UniGeneiRn.11110

Genome annotation databases

GeneIDi116593
KEGGirno:116593

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97662 mRNA Translation: AAA40804.1
BC078767 mRNA Translation: AAH78767.1
PIRiA46624 S27881
RefSeqiNP_446297.1, NM_053845.1
UniGeneiRn.11110

3D structure databases

ProteinModelPortaliQ03248
SMRiQ03248
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ03248
PhosphoSitePlusiQ03248

Proteomic databases

PRIDEiQ03248

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116593
KEGGirno:116593

Organism-specific databases

CTDi51733
RGDi620091 Upb1

Phylogenomic databases

HOVERGENiHBG018848
InParanoidiQ03248
KOiK01431
PhylomeDBiQ03248

Enzyme and pathway databases

UniPathwayi
UPA00131

BioCyciMetaCyc:MONOMER-15401
SABIO-RKiQ03248

Miscellaneous databases

PROiPR:Q03248

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBUP1_RAT
AccessioniPrimary (citable) accession number: Q03248
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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