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Protein

Ras-related protein ced-10

Gene

ced-10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required in engulfing to control the phagocytosis of apoptotic cell corpses (PubMed:10707082, PubMed:20126385). Required in embryonic development for the correct positioning and orientation of the mitotic spindles and division planes in blastomere cells (PubMed:20126385). Involved in hypodermal cell fusion, together with pak-1 and cdc-42, leading to embryonic body elongation, which involves dramatic cytoskeletal reorganization (PubMed:8824291). Ced-2 and ced-5 function to activate ced-10 in a GTPase signaling pathway that controls the polarized extension of cell surfaces (PubMed:10707082). Plays a redundant role with mig-2 in dorsal axonal guidance in ventral cord commissural motoneurons and in P neuroblast migration. May regulate these 2 processes by activating pak-1 and/or max-2 (PubMed:17050621). Plays a role, probably via mig-10, in orientating axonal growth of HSN and AVM neurons in response to guidance cues such as slt-1. Regulates mig-10 asymmetric distribution in HSN neurons (PubMed:18499456). During gonad morphogenesis, plays a role in distal tip cell (DTC)-mediated guidance of gonad elongation, probably by activating max-2 (PubMed:19797046, PubMed:19023419). Furthermore, plays a role in distal tip cell polarity and migration by negatively regulating the unc-6/Netrin receptor unc-5 (PubMed:26292279). May be involved in signal transduction during cell migration (PubMed:10707082). May be involved in the positioning of ray 1, the most anterior ray sensilium, in the male tail (PubMed:24004945).9 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi115 – 118GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processApoptosis, Neurogenesis, Phagocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114604 GPVI-mediated activation cascade
R-CEL-1433557 Signaling by SCF-KIT
R-CEL-193648 NRAGE signals death through JNK
R-CEL-194840 Rho GTPase cycle
R-CEL-2029482 Regulation of actin dynamics for phagocytic cup formation
R-CEL-389359 CD28 dependent Vav1 pathway
R-CEL-3928662 EPHB-mediated forward signaling
R-CEL-3928664 Ephrin signaling
R-CEL-3928665 EPH-ephrin mediated repulsion of cells
R-CEL-4086400 PCP/CE pathway
R-CEL-416482 G alpha (12/13) signalling events
R-CEL-418885 DCC mediated attractive signaling
R-CEL-4420097 VEGFA-VEGFR2 Pathway
R-CEL-445144 Signal transduction by L1
R-CEL-5218920 VEGFR2 mediated vascular permeability
R-CEL-5625740 RHO GTPases activate PKNs
R-CEL-5626467 RHO GTPases activate IQGAPs
R-CEL-5627123 RHO GTPases activate PAKs
R-CEL-5663213 RHO GTPases Activate WASPs and WAVEs
R-CEL-5687128 MAPK6/MAPK4 signaling
R-CEL-6798695 Neutrophil degranulation
R-CEL-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-CEL-8875555 MET activates RAP1 and RAC1
R-CEL-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiQ03206

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein ced-10
Alternative name(s):
CErac1
Cell death protein 10
Cell-corpse engulfment protein ced-10
Ras-related protein rac-1
Gene namesi
Name:ced-10
Synonyms:rac-1
ORF Names:C09G12.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC09G12.8a ; CE16832 ; WBGene00000424 ; ced-10
C09G12.8b ; CE16833 ; WBGene00000424 ; ced-10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

In the second generation, there is defective mitotic spindle orientation in the EMS and ABar blastomeres which results in disrupted left-right asymmetry and failure to undergo morphogenesis (PubMed:20126385). Due to defective apoptotic cell clearance, embryos accumulate apoptotic cell corpses (PubMed:20126385). Distal tip cell migratory defects (PubMed:26292279). Double knockout with unc-5 RNAi suppresses the distal tip cell migratory defect in the ced-10 single mutant (PubMed:26292279).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12G → V: May be constitutively active. Formation of ectopic processes often branched in PDE neurons. 1 Publication1
Mutagenesisi60G → R in n3246; in HSN neurons, severe reduction in mig-10 ventral enrichment and mild defect in axonal guidance but normal final migration to the ventral nerve cord. 1 Publication1
Mutagenesisi61Q → L: May lock enzyme in its GTP-bound active state. Defect in distal tip cell (DTC) migration. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988911 – 188Ras-related protein ced-10Add BLAST188
PropeptideiPRO_0000281242189 – 191Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Cysteine methyl esterBy similarity1
Lipidationi188S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ03206
PaxDbiQ03206
PeptideAtlasiQ03206
PRIDEiQ03206

Expressioni

Tissue specificityi

Colocalizes with pak-1 to hypodermal cell boundaries during embryo elongation throughout the second phase of embryogenesis.1 Publication

Developmental stagei

Most abundant at embryonic stage, its expression decreases dramatically during development.1 Publication

Gene expression databases

BgeeiWBGene00000424 Expressed in 5 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Interactioni

Subunit structurei

Interacts (GTP-bound form) with pak-1 (PubMed:8824291). May interact (GTP-bound form) with mig-10 (via Ras-associating and PH domains) (PubMed:18499456).2 Publications

Protein-protein interaction databases

BioGridi42251, 14 interactors
IntActiQ03206, 1 interactor
STRINGi6239.C09G12.8b.1

Structurei

3D structure databases

ProteinModelPortaliQ03206
SMRiQ03206
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
InParanoidiQ03206
KOiK04392
OMAiKAKWFPE
OrthoDBiEOG091G0KCM
PhylomeDBiQ03206

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform a (identifier: Q03206-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGRP
60 70 80 90 100
INLGLWDTAG QEDYDRLRPL SYPQTDVFLV CFALNNPASF ENVRAKWYPE
110 120 130 140 150
VSHHCPNTPI ILVGTKADLR EDRDTVERLR ERRLQPVSQT QGYVMAKEIK
160 170 180 190
AVKYLECSAL TQRGLKQVFD EAIRAVLTPP QRAKKSKCTV L
Length:191
Mass (Da):21,455
Last modified:June 6, 2002 - v2
Checksum:i1DE85C308B996AFB
GO
Isoform b (identifier: Q03206-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-130: Missing.

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):14,332
Checksum:i06BE559FFEFDE383
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177L → V in AAA28140 (PubMed:7677998).Curated1
Sequence conflicti177L → V in AAA28141 (PubMed:7677998).Curated1
Sequence conflicti177L → V in CAA48506 (PubMed:7677998).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00571169 – 130Missing in isoform b. CuratedAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03711 mRNA Translation: AAA28140.1
L04287 mRNA Translation: AAA28141.1
X68492 mRNA Translation: CAA48506.1
AF226867 mRNA Translation: AAF33846.1
FO080481 Genomic DNA Translation: CCD64025.1
FO080481 Genomic DNA Translation: CCD64024.1
PIRiA45324
G88650
RefSeqiNP_500362.3, NM_067961.4 [Q03206-2]
NP_500363.1, NM_067962.5 [Q03206-1]
UniGeneiCel.19354

Genome annotation databases

EnsemblMetazoaiC09G12.8b; C09G12.8b; WBGene00000424 [Q03206-1]
GeneIDi177111
KEGGicel:CELE_C09G12.8
UCSCiC09G12.8b c. elegans [Q03206-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAC1_CAEEL
AccessioniPrimary (citable) accession number: Q03206
Secondary accession number(s): O44463, O44464
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 6, 2002
Last modified: September 12, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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