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UniProtKB - Q03194 (PMA4_NICPL)
Protein
Plasma membrane ATPase 4
Gene
PMA4
Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Functioni
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 332 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 591 | MagnesiumBy similarity | 1 | |
Metal bindingi | 595 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- P-type proton-exporting transporter activity Source: UniProtKB-EC
GO - Biological processi
- proton export across plasma membrane Source: InterPro
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PMA4 |
Organismi | Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco) |
Taxonomic identifieri | 4092 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 64 | CytoplasmicSequence analysisAdd BLAST | 64 | |
Transmembranei | 65 – 84 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 85 – 96 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 97 – 117 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 246 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 247 – 267 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 268 – 277 | ExtracellularSequence analysis | 10 | |
Transmembranei | 278 – 299 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 300 – 646 | CytoplasmicSequence analysisAdd BLAST | 347 | |
Transmembranei | 647 – 668 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 669 – 673 | ExtracellularSequence analysis | 5 | |
Transmembranei | 674 – 696 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 697 – 712 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 713 – 733 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 734 – 754 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 755 – 775 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 776 – 787 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 788 – 808 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 809 – 817 | ExtracellularSequence analysis | 9 | |
Transmembranei | 818 – 838 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 839 – 952 | CytoplasmicSequence analysisAdd BLAST | 114 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046293 | 1 – 952 | Plasma membrane ATPase 4Add BLAST | 952 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q03194 |
Expressioni
Tissue specificityi
Expressed at high levels in root, stem, leaf and flower.
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03194-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKAISLEEI KNETVDLEKI PIEEVFEQLK CTREGLSADE GASRLQIFGP
60 70 80 90 100
NKLEEKNESK ILKFLGFMWN PLSWVMEAAA VMAIALANGD GKPPDWQDFI
110 120 130 140 150
GIICLLVINS TISFIEENNA GNAAAALMAG LAPKTKVLRD GRWSEQEAAI
160 170 180 190 200
LVPGDIISVK LGDIIPADAR LLEGDPLKID QSALTGESLP VTKNPGDEVF
210 220 230 240 250
SGSTCKQGEL EAVVIATGVH TFFGKAAHLV DSTNNVGHFQ KVLTAIGNFC
260 270 280 290 300
ICSIAIGMLV EIIVMYPIQH RKYRDGIDNL LVLLIGGIPI AMPTVLSVTM
310 320 330 340 350
AIGSHRLSQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDRNLVE
360 370 380 390 400
VFAKGVDKEY VLLLAARASR VENQDAIDAC MVGMLADPKE ARAGIREVHF
410 420 430 440 450
LPFNPVDKRT ALTYIDNNNN WHRASKGAPE QILDLCNAKE DVRRKVHSMM
460 470 480 490 500
DKYAERGLRS LAVARRTVPE KSKESPGGRW EFVGLLPLFD PPRHDSAETI
510 520 530 540 550
RRALNLGVNV KMITGDQLAI AKETGRRLGM GTNMYPSASL LGQDKDSAIA
560 570 580 590 600
SLPIEELIEK ADGFAGVFPE HKYEIVKKLQ ERKHIVGMTG DGVNDAPALK
610 620 630 640 650
KADIGIAVAD ATDAARGASD IVLTEPGLSV IISAVLTSRA IFQRMKNYTI
660 670 680 690 700
YAVSITIRIV FGFMFIALIW KYDFSAFMVL IIAILNDGTI MTISKDRVKP
710 720 730 740 750
SPMPDSWKLK EIFATGVVLG GYQALMTVVF FWAMHDTDFF SDKFGVKSLR
760 770 780 790 800
NSDEEMMSAL YLQVSIISQA LIFVTRSRSW SFLERPGMLL VIAFMIAQLV
810 820 830 840 850
ATLIAVYANW AFARVKGCGW GWAGVIWLYS IIFYLPLDIM KFAIRYILSG
860 870 880 890 900
KAWNNLLDNK TAFTTKKDYG KEEREAQWAL AQRTLHGLQP PEATNLFNEK
910 920 930 940 950
NSYRELSEIA EQAKRRAEMA RLRELHTLKG HVESVVKLKG LDIETIQQHY
TV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66737 mRNA Translation: CAA47275.1 |
PIRi | S33548 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66737 mRNA Translation: CAA47275.1 |
PIRi | S33548 |
3D structure databases
AlphaFoldDBi | Q03194 |
SMRi | Q03194 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q03194 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA4_NICPL | |
Accessioni | Q03194Primary (citable) accession number: Q03194 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |