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UniProtKB - Q03149 (WA_EMENI)
Protein
Conidial yellow pigment biosynthesis polyketide synthase
Gene
wA
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Functioni
A polyketide synthase which condenses acetate units to form a heptaketide naphthopyrene YWA1, via a polyketomethylene intermediate step. YWA1 is a yellow pigment found in mature asexual spores (conidia).
3 PublicationsCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
Cofactori
pantetheine 4'-phosphateNote: Binds 2 phosphopantetheines covalently.
: heptaketide naphthopyrone YWA1 biosynthesis Pathwayi
This protein is involved in the pathway heptaketide naphthopyrone YWA1 biosynthesis, which is part of Polyketide biosynthesis.View all proteins of this organism that are known to be involved in the pathway heptaketide naphthopyrone YWA1 biosynthesis and in Polyketide biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 548 | For beta-ketoacyl synthase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1001 | For acyl/malonyl transferase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1967 | For thioesterase activity | 1 |
GO - Molecular functioni
- 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: InterPro
- hydroquinone:oxygen oxidoreductase activity Source: AspGD
- phosphopantetheine binding Source: InterPro
GO - Biological processi
- conidium formation Source: UniProtKB-KW
- fatty acid biosynthetic process Source: InterPro
- pigment biosynthetic process Source: AspGD
- pigment metabolic process involved in developmental pigmentation Source: AspGD
- secondary metabolic process Source: AspGD
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Multifunctional enzyme, Transferase |
Biological process | Conidiation, Sporulation |
Enzyme and pathway databases
UniPathwayi | UPA00167 |
Names & Taxonomyi
Protein namesi | Recommended name: Conidial yellow pigment biosynthesis polyketide synthase1 Publication (EC:2.3.1.-1 Publication)Short name: PKS1 Publication |
Gene namesi | Name:wA ORF Names:AN8209 |
Organismi | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
Taxonomic identifieri | 227321 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Nidulantes › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:AN8209 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1682 | S → A: Fails to form the naphthopyrene YWA1; when associated with A-1804. 1 Publication | 1 | |
Mutagenesisi | 1682 | S → C or A: Forms the naphthopyrene YWA1. 1 Publication | 1 | |
Mutagenesisi | 1682 | S → C: Fails to form the naphthopyrene YWA1; when associated with C-1804. 1 Publication | 1 | |
Mutagenesisi | 1804 | S → A: Fails to form the naphthopyrene YWA1; when associated with A-1682. 1 Publication | 1 | |
Mutagenesisi | 1804 | S → C or A: Forms the naphthopyrene YWA1. 1 Publication | 1 | |
Mutagenesisi | 1804 | S → C: Fails to form the naphthopyrene YWA1; when associated with C-1682. 1 Publication | 1 | |
Mutagenesisi | 1967 | S → A: Required for Claisen-type cyclization of the final product. 1 Publication | 1 | |
Mutagenesisi | 2129 | H → Q: Required for Claisen-type cyclization of the final product. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180305 | 1 – 2157 | Conidial yellow pigment biosynthesis polyketide synthaseAdd BLAST | 2157 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1682 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 | |
Modified residuei | 1804 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1645 – 1722 | Carrier 1PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 1767 – 1844 | Carrier 2PROSITE-ProRule annotationAdd BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 529 – 582 | Beta-ketoacyl synthaseBy similarityAdd BLAST | 54 | |
Regioni | 991 – 1024 | Acyl/malonyl transferasesBy similarityAdd BLAST | 34 | |
Regioni | 1607 – 1638 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 1725 – 1760 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 1847 – 1888 | DisorderedSequence analysisAdd BLAST | 42 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1608 – 1624 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 1851 – 1888 | Polar residuesSequence analysisAdd BLAST | 38 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1202, Eukaryota |
HOGENOMi | CLU_000022_16_2_1 |
InParanoidi | Q03149 |
OMAi | VCVYGLN |
OrthoDBi | 68112at2759 |
Family and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.10.129.110, 1 hit 3.40.366.10, 2 hits 3.40.47.10, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR030918, PT_fungal_PKS IPR032088, SAT IPR001031, Thioesterase IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF00550, PP-binding, 2 hits PF14765, PS-DH, 1 hit PF16073, SAT, 1 hit PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF52151, SSF52151, 1 hit SSF53474, SSF53474, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
TIGRFAMsi | TIGR04532, PT_fungal_PKS, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03149-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDPYRVYLF GDQTGDFEVG LRRLLQAKNH SLLSSFLQRS YHAVRQEISH
60 70 80 90 100
LPPSERSTFP RFTSIGDLLA RHCESPGNPA IESVLTCIYQ LGCFINYYGD
110 120 130 140 150
LGHTFPSHSQ SQLVGLCTGL LSCAAVSCAS NIGELLKPAV EVVVVALRLG
160 170 180 190 200
LCVYRVRKLF GQDQAAPLSW SALVSGLSES EGTSLIDKFT RRNVIPPSSR
210 220 230 240 250
PYISAVCANT LTISGPPVVL NQFLDTFISG KNKAVMVPIH GPFHASHLYE
260 270 280 290 300
KRDVEWILKS CNVETIRNHK PRIPVLSSNT GELIVVENME GFLKIALEEI
310 320 330 340 350
LLRQMSWDKV TDSCISILKS VGDNKPKKLL PISSTATQSL FNSLKKSNLV
360 370 380 390 400
NIEVDGGISD FAAETQLVNQ TGRAELSKIA IIGMSGRFPE ADSPQDFWNL
410 420 430 440 450
LYKGLDVHRK VPEDRWDADA HVDLTGTATN TSKVPYGCWI REPGLFDPRF
460 470 480 490 500
FNMSPREALQ ADPAQRLALL TAYEALEGAG FVPDSTPSTQ RDRVGIFYGM
510 520 530 540 550
TSDDYREVNS GQDIDTYFIP GGNRAFTPGR INYYFKFSGP SVSVDTACSS
560 570 580 590 600
SLAAIHLACN SIWRNDCDTA ITGGVNILTN PDNHAGLDRG HFLSRTGNCN
610 620 630 640 650
TFDDGADGYC RADGVGTVVL KRLEDALADN DPILGVINGA YTNHSAEAVS
660 670 680 690 700
ITRPHVGAQA FIFKKLLNEA NVDPKNISYI EMHGTGTQAG DAVEMQSVLD
710 720 730 740 750
VFAPDHRRGP GQSLHLGSAK SNIGHGESAS GVTSLVKVLL MMKENMIPPH
760 770 780 790 800
CGIKTKINHN FPTDLAQRNV HIALQPTAWN RPSFGKRQIF LNNFSAAGGN
810 820 830 840 850
TALLLEDGPV SDPEGEDKRR THVITLSARS QTALQNNIDA LCQYISEQEK
860 870 880 890 900
TFGVKDSNAL PSLAYTTTAR RIHHPFRVTA IGSSFQEMRD SLIASSRKEF
910 920 930 940 950
VAVPAKTPGI GFLFTGQGAQ YAAMGKQLYE DCSHFRSAIE HLDCISQGQD
960 970 980 990 1000
LPSILPLVDG SLPLSELSPV VVQLGTTCVQ MALSSFWASL GITPSFVLGH
1010 1020 1030 1040 1050
SLGDFAAMNA AGVLSTSDTI YACGRRAQLL TERCQPGTHA MLAIKAPLVE
1060 1070 1080 1090 1100
VKQLLNEKVH DMACINSPSE TVISGPKSSI DELSRACSEK GLKSTILTVP
1110 1120 1130 1140 1150
YAFHSAQVEP ILEDLEKALQ GITFNKPSVP FVSALLGEVI TEAGSNILNA
1160 1170 1180 1190 1200
EYLVRHCRET VNFLSAFEAV RNAKLGGDQT LWLEVGPHTV CSGMVKATLG
1210 1220 1230 1240 1250
PQTTTMASLR RDEDTWKVLS NSLSSLYLAG VDINWKQYHQ DFSSSHRVLP
1260 1270 1280 1290 1300
LPTYKWDLKN YWIPYRNNFC LTKGSSMSAA SASLQPTFLT TSAQRVVESR
1310 1320 1330 1340 1350
DDGLTATVVV HNDIADPDLN RVIQGHKVNG AALCPSSLYA DSAQTLAEYL
1360 1370 1380 1390 1400
IEKYKPELKG SGLDVCNVTV PKPLIAKTGK EQFRISATAN WVDKHVSVQV
1410 1420 1430 1440 1450
FSVTAEGKKL IDHAHCEVKL FDCMAADLEW KRGSYLVKRS IELLENSAVK
1460 1470 1480 1490 1500
GDAHRLRRGM VYKLFSALVD YDENYQSIRE VILDSEHHEA TALVKFQAPQ
1510 1520 1530 1540 1550
ANFHRNPYWI DSFGHLSGFI MNASDGTDSK SQVFVNHGWD SMRCLKKFSA
1560 1570 1580 1590 1600
DVTYRTYVRM QPWRDSIWAG NVYIFEGDDI IAVFGGVKFQ ALSRKILDIA
1610 1620 1630 1640 1650
LPPAGLSKAQ TSPIQSSAPQ KPIETAKPTS RPAPPVTMKS FVKKSAGPSV
1660 1670 1680 1690 1700
VVRALNILAS EVGLSESDMS DDLVFADYGV DSLLSLTVTG KYREELNLDM
1710 1720 1730 1740 1750
DSSVFIEHPT VGDFKRFVTQ LSPSVASDSS STDRESEYSF NGDSCSGLSS
1760 1770 1780 1790 1800
PASPGTVSPP NEKVIQIHEN GTMKEIRAII ADEIGVSADE IKSDENLNEL
1810 1820 1830 1840 1850
GMDSLLSLTV LGKIRESLDM DLPGEFFIEN QTLDQIETAL DLKPKAVPTA
1860 1870 1880 1890 1900
VPQSQPITLP QSQSTKQLST RPTSSSDNHP PATSILLQGN PRTASKTLFL
1910 1920 1930 1940 1950
FPDGSGSATS YATIPGVSPN VAVYGLNCPY MKAPEKLTCS LDSLTTPYLA
1960 1970 1980 1990 2000
EIRRRQPTGP YNLGGWSAGG ICAYDAARKL VLQQGEIVET LLLLDTPFPI
2010 2020 2030 2040 2050
GLEKLPPRLY SFFNSIGLFG EGKAAPPAWL LPHFLAFIDS LDAYKAVPLP
2060 2070 2080 2090 2100
FNEQEWKGKL PKTYLVWAKD GVCPKPGDPW PEPAEDGSKD PREMVWLLSN
2110 2120 2130 2140 2150
RTDLGPNGWD TLVGKENIGG ITVIHDANHF TMTKGEKAKE LATFMKNALG
VCERRLV
Sequence cautioni
The sequence EAA58778 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65866 Genomic DNA Translation: CAA46695.2 AACD01000143 Genomic DNA Translation: EAA58778.1 Sequence problems. BN001302 Genomic DNA Translation: CBF74114.1 |
PIRi | S28353 |
RefSeqi | XP_681478.1, XM_676386.1 |
Genome annotation databases
EnsemblFungii | CBF74114; CBF74114; ANIA_08209 EAA58778; EAA58778; AN8209.2 |
GeneIDi | 2868945 |
KEGGi | ani:AN8209.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65866 Genomic DNA Translation: CAA46695.2 AACD01000143 Genomic DNA Translation: EAA58778.1 Sequence problems. BN001302 Genomic DNA Translation: CBF74114.1 |
PIRi | S28353 |
RefSeqi | XP_681478.1, XM_676386.1 |
3D structure databases
AlphaFoldDBi | Q03149 |
SMRi | Q03149 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 162425.CADANIAP00004256 |
Genome annotation databases
EnsemblFungii | CBF74114; CBF74114; ANIA_08209 EAA58778; EAA58778; AN8209.2 |
GeneIDi | 2868945 |
KEGGi | ani:AN8209.2 |
Organism-specific databases
VEuPathDBi | FungiDB:AN8209 |
Phylogenomic databases
eggNOGi | KOG1202, Eukaryota |
HOGENOMi | CLU_000022_16_2_1 |
InParanoidi | Q03149 |
OMAi | VCVYGLN |
OrthoDBi | 68112at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00167 |
Family and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.10.129.110, 1 hit 3.40.366.10, 2 hits 3.40.47.10, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR030918, PT_fungal_PKS IPR032088, SAT IPR001031, Thioesterase IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF00550, PP-binding, 2 hits PF14765, PS-DH, 1 hit PF16073, SAT, 1 hit PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF52151, SSF52151, 1 hit SSF53474, SSF53474, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
TIGRFAMsi | TIGR04532, PT_fungal_PKS, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WA_EMENI | |
Accessioni | Q03149Primary (citable) accession number: Q03149 Secondary accession number(s): C8V774, Q5AU21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | March 1, 2004 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways