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Protein

Pyridoxal 5'-phosphate synthase subunit SNO1

Gene

SNO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of a SNZ isoform.Curated

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.Curated
L-glutamine + H2O = L-glutamate + NH3.1 Publication

Kineticsi

  1. KM=3.4 mM for glutamine1 Publication
  1. Vmax=0.48 µmol/min/mg enzyme with glutamine as substrate1 Publication

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100NucleophileBy similarity1
Binding sitei129L-glutamineBy similarity1
Active sitei203Charge relay systemBy similarity1
Active sitei205Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: SGD
  • pyridoxal phosphate biosynthetic process Source: GO_Central
  • pyridoxine biosynthetic process Source: SGD

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32795-MONOMER
YEAST:G3O-32795-MONOMER
UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit SNO1 (EC:4.3.3.6)
Alternative name(s):
PDX2 homolog 1
Short name:
Pdx2.1
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.21 Publication)
Gene namesi
Name:SNO1
Ordered Locus Names:YMR095C
ORF Names:YM6543.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR095C
SGDiS000004701 SNO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356191 – 224Pyridoxal 5'-phosphate synthase subunit SNO1Add BLAST224

Proteomic databases

MaxQBiQ03144
PaxDbiQ03144
PRIDEiQ03144

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SNZ1Q031483EBI-28190,EBI-17618

Protein-protein interaction databases

BioGridi35270, 55 interactors
ComplexPortaliCPX-1370 SNO1-SNZ1 pyridoxal 5'-phosphate synthase complex
DIPiDIP-1642N
IntActiQ03144, 10 interactors
MINTiQ03144
STRINGi4932.YMR095C

Structurei

3D structure databases

ProteinModelPortaliQ03144
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 69L-glutamine bindingBy similarity3
Regioni160 – 161L-glutamine bindingBy similarity2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00390000011516
HOGENOMiHOG000039949
InParanoidiQ03144
KOiK08681
OMAiVFIRAPI
OrthoDBiEOG092C4BRK

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKTHSTMSG KSMKVIGVLA LQGAFLEHTN HLKRCLAEND YGIKIEIKTV
60 70 80 90 100
KTPEDLAQCD ALIIPGGEST SMSLIAQRTG LYPCLYEFVH NPEKVVWGTC
110 120 130 140 150
AGLIFLSAQL ENESALVKTL GVLKVDVRRN AFGRQAQSFT QKCDFSNFIP
160 170 180 190 200
GCDNFPATFI RAPVIERILD PIAVKSLYEL PVNGKDVVVA ATQNHNILVT
210 220
SFHPELADSD TRFHDWFIRQ FVSN
Length:224
Mass (Da):24,907
Last modified:November 1, 1997 - v1
Checksum:iC0EC1B2382581D40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49807 Genomic DNA Translation: CAA89896.1
AY692745 Genomic DNA Translation: AAT92764.1
BK006946 Genomic DNA Translation: DAA09992.1
PIRiS55081
RefSeqiNP_013813.1, NM_001182595.1

Genome annotation databases

EnsemblFungiiYMR095C; YMR095C; YMR095C
GeneIDi855120
KEGGisce:YMR095C

Similar proteinsi

Entry informationi

Entry nameiSNO1_YEAST
AccessioniPrimary (citable) accession number: Q03144
Secondary accession number(s): D6VZR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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