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Protein

Adenylate cyclase, germination specific

Gene

acgA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Has a large extracellular domain which may be involved in the recognition of an extracellular signal present during germination, leading to activation or inhibition of cAMP synthesis by the cytoplasmic domain.

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Enzyme regulationi

Insensitive to guanine nucleotides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi401Magnesium 1PROSITE-ProRule annotation1
Metal bindingi401Magnesium 2PROSITE-ProRule annotation1
Metal bindingi402Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi445Magnesium 1PROSITE-ProRule annotation1
Metal bindingi445Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • adenylate cyclase activity Source: dictyBase
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • osmosensor activity Source: dictyBase
  • protein homodimerization activity Source: dictyBase

GO - Biological processi

  • cAMP biosynthetic process Source: dictyBase
  • cellular response to osmotic stress Source: dictyBase
  • intracellular signal transduction Source: InterPro
  • negative regulation of spore germination Source: dictyBase
  • positive regulation of cAMP-dependent protein kinase activity Source: dictyBase
  • regulation of protein kinase activity Source: dictyBase
  • response to acidic pH Source: dictyBase
  • response to caffeine Source: dictyBase
  • response to osmotic stress Source: dictyBase
  • signal transduction Source: dictyBase
  • spore germination Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • water homeostasis Source: dictyBase

Keywordsi

Molecular functionLyase
Biological processcAMP biosynthesis, Germination
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-163359 Glucagon signaling in metabolic regulation
R-DDI-163615 PKA activation
R-DDI-164378 PKA activation in glucagon signalling
R-DDI-170660 Adenylate cyclase activating pathway
R-DDI-170670 Adenylate cyclase inhibitory pathway
R-DDI-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-DDI-418555 G alpha (s) signalling events
R-DDI-418597 G alpha (z) signalling events
R-DDI-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-DDI-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-DDI-5610787 Hedgehog 'off' state

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase, germination specific (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase
Gene namesi
Name:acgA
Synonyms:acg
ORF Names:DDB_G0274569
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274569 acgA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 41Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini42 – 858ExtracellularSequence analysisAdd BLAST817

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: dictyBase
  • plasma membrane Source: GO_Central

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001957151 – 858Adenylate cyclase, germination specificAdd BLAST858

Proteomic databases

PaxDbiQ03101
PRIDEiQ03101

Expressioni

Developmental stagei

After fruiting bodies have been formed and during germination.

Interactioni

GO - Molecular functioni

  • protein homodimerization activity Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0185013

Structurei

3D structure databases

ProteinModelPortaliQ03101
SMRiQ03101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 317CHASEPROSITE-ProRule annotationAdd BLAST232
Domaini396 – 526Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi738 – 848Asn-richAdd BLAST111
Compositional biasi835 – 848Poly-AsnAdd BLAST14

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
InParanoidiQ03101
OMAiERSNNAC

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR006189 CHASE_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF03924 CHASE, 1 hit
PF00211 Guanylate_cyc, 1 hit
SMARTiView protein in SMART
SM01079 CHASE, 1 hit
SM00044 CYCc, 1 hit
SUPFAMiSSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS50839 CHASE, 1 hit
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTFVKILS KSYVEGYPVG FFIGLIILAI FGSMVCIFSF MHYSEEENSN
60 70 80 90 100
IQMDLERSSK QIIHNIQMNA MYLLSSIDTL KALYYVNPNF DRNDFNVFLN
110 120 130 140 150
TTLKNSEFQY LFWIKKINNN DRNCFEEKFS KEIKDTFQIY SFDENTNSIH
160 170 180 190 200
VAKNKSSYFP ILHAFPDINK DIIGLDINST DYMNETIKKS IFNKKPTVHL
210 220 230 240 250
NKKVLLSKRN IDILIVSPII VTKTLESTNE TMEDMSHISS GLFLMEKNVQ
260 270 280 290 300
ASRIEVENGN DFTIFLSTTN GEIVYQENYL NFKTLYQVHE SGLFEDRLKY
310 320 330 340 350
ESSLKIADCV LKLWIFTTEE YENNSKTYLP LLVSIISAVI LVLLITYSVD
360 370 380 390 400
QRKQKSLIAK IMREKNNLIN KILPLEVSVK LENGEDVVAE RSNNACVFFL
410 420 430 440 450
DIAGFTRFSS IHSPEQVIQV LIKIFNSMDL LCAKHGIEKI KTIGDAYMAT
460 470 480 490 500
CGIFPKCDDI RHNTYKMLGF AMDVLEFIPK EMSFHLGLQV RVGIHCGPVI
510 520 530 540 550
SGVISGYAKP HFDVWGDTVN VASRMESTGI AGQIHVSDRV YQLGKEDFNF
560 570 580 590 600
SERCDIIHVK GKGRMKTWYL MGKKSSDFSL KKDFSRSRVQ PSLFNRKQSH
610 620 630 640 650
VHCIYPEFPS GLQALNIENN LNNTDAGCEN CSKILKKTYA YSPDHSTSNY
660 670 680 690 700
YYHGDDNSPP PPSLNSNDLI DGSEYHDDPF PSDSNVGYHD TSKDIKEDEN
710 720 730 740 750
EQNETLLFNQ EQLKKKQIEN IQRDLSLNDS IEAIKILNNN NNNNINDNNI
760 770 780 790 800
NNTNLNNNNN DININNSDNV NNYENNNNFS DKIENNDGDN NNINDNNYKS
810 820 830 840 850
TNENNIKSKT LFKDSKSLIN DIKMAKENCD NNDDNNNNNN NNNNNNNNDE

NVESKKNK
Length:858
Mass (Da):98,380
Last modified:December 4, 2007 - v2
Checksum:iB409D387D8A96453
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti755L → F in AAA33164 (PubMed:1348970).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87278 Genomic DNA Translation: AAA33164.1
AAFI02000012 Genomic DNA Translation: EAL70177.1
PIRiA42239
RefSeqiXP_643956.1, XM_638864.1

Genome annotation databases

EnsemblProtistsiEAL70177; EAL70177; DDB_G0274569
GeneIDi8619384
KEGGiddi:DDB_G0274569

Similar proteinsi

Entry informationi

Entry nameiCYAG_DICDI
AccessioniPrimary (citable) accession number: Q03101
Secondary accession number(s): Q555V4, Q86A89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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