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UniProtKB - Q03070 (CHIO_RAT)
Protein
Beta-chimaerin
Gene
Chn2
Organism
Rattus norvegicus (Rat)
Status
Functioni
GTPase-activating protein for p21-rac.
Activity regulationi
In the inactive state, the N terminus protrudes into the active site of the Rho-GAP domain, sterically blocking Rac binding. Phospholipid binding to the Phorbol-ester/DAG-type zinc-finger/C1 domain triggers the cooperative dissociation of these interactions, allowing the N-terminus to move out of the active site and thereby activating the enzyme (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 91 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 |
GO - Molecular functioni
- GTPase activator activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- phorbol ester receptor activity Source: RGD
GO - Biological processi
- acrosome assembly Source: RGD
- intracellular signal transduction Source: InterPro
- positive regulation of GTPase activity Source: InterPro
- regulation of GTPase activity Source: GO_Central
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-9013149, RAC1 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-chimaerinAlternative name(s): Beta-chimerin Rho GTPase-activating protein 3 |
Gene namesi | Name:Chn2 Synonyms:Arhgap3, Bch |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620140, Chn2 |
Subcellular locationi
Other locations
- Membrane Curated; Peripheral membrane protein Curated
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056699 | 1 – 295 | Beta-chimaerinAdd BLAST | 295 |
Proteomic databases
PaxDbi | Q03070 |
Expressioni
Tissue specificityi
Found in cerebellum and testis.
Developmental stagei
Expressed specifically in late stage spermatocytes. In the cerebellum, emergence of beta-2 isoform coincides with granule cells maturation and exhibits postnatal developmental increases. Expression is specifically reduced in weaver mutant.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 295 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 192 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 91 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
SH2 domain, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1453, Eukaryota |
InParanoidi | Q03070 |
OrthoDBi | 1300981at2759 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd04372, RhoGAP_chimaerin, 1 hit |
Gene3Di | 1.10.555.10, 1 hit |
InterProi | View protein in InterPro IPR020454, DAG/PE-bd IPR002219, PE/DAG-bd IPR008936, Rho_GTPase_activation_prot IPR037860, RhoGAP_chimaerin IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00620, RhoGAP, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50238, RHOGAP, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform Beta-1 (identifier: Q03070-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLCTSPVNCL DSVCCTSINI SSLVRRAALT HNDNHFNYEK THNFKVHTFR
60 70 80 90 100
GPHWCEYCAN FMWGLIAQGV RCSDCGLNVH KQCSKHVPND CQPDLKRIKK
110 120 130 140 150
VYCCDLTTLV KAHNTQRPMV VDICIREIEA RGLKSEGLYR VSGFTEHIED
160 170 180 190 200
VKMAFDRDGE KADISANIYP DINIITGALK LYFRDLPIPI ITYDTYTKFI
210 220 230 240 250
EAAKISNADE RLEAVHEVLM LLPPAHYETL RYLMIHLKKV TMNEKDNLMN
260 270 280 290
AENLGIVFGP TLMRPPEDST LTTLHDMRYQ KLIVQILIEN EDVLF
Isoform Beta-2 (identifier: Q03070-2)
Sequence is not available
Length:–
Mass (Da):–
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JXD5 | A0A0G2JXD5_RAT | Chimaerin | Chn2 | 460 | Annotation score: | ||
G3V773 | G3V773_RAT | Beta-chimaerin | Chn2 rCG_52339 | 295 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69489 mRNA Translation: CAA49244.1 L07494 mRNA Translation: AAA40809.1 |
PIRi | A45485, S29956 |
RefSeqi | NP_114473.1, NM_032084.1 [Q03070-1] |
Genome annotation databases
GeneIDi | 84031 |
KEGGi | rno:84031 |
UCSCi | RGD:620140, rat [Q03070-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69489 mRNA Translation: CAA49244.1 L07494 mRNA Translation: AAA40809.1 |
PIRi | A45485, S29956 |
RefSeqi | NP_114473.1, NM_032084.1 [Q03070-1] |
3D structure databases
SMRi | Q03070 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000012655 |
Proteomic databases
PaxDbi | Q03070 |
Genome annotation databases
GeneIDi | 84031 |
KEGGi | rno:84031 |
UCSCi | RGD:620140, rat [Q03070-1] |
Organism-specific databases
CTDi | 1124 |
RGDi | 620140, Chn2 |
Phylogenomic databases
eggNOGi | KOG1453, Eukaryota |
InParanoidi | Q03070 |
OrthoDBi | 1300981at2759 |
Enzyme and pathway databases
Reactomei | R-RNO-9013149, RAC1 GTPase cycle |
Miscellaneous databases
PROi | PR:Q03070 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd04372, RhoGAP_chimaerin, 1 hit |
Gene3Di | 1.10.555.10, 1 hit |
InterProi | View protein in InterPro IPR020454, DAG/PE-bd IPR002219, PE/DAG-bd IPR008936, Rho_GTPase_activation_prot IPR037860, RhoGAP_chimaerin IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00620, RhoGAP, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50238, RHOGAP, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHIO_RAT | |
Accessioni | Q03070Primary (citable) accession number: Q03070 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |