UniProtKB - Q03043 (KGP24_DROME)
cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B
for
Functioni
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 699 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 807 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 901 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 584 – 587 | 3',5'-cGMP 1By similarity | 4 | |
Nucleotide bindingi | 594 – 595 | 3',5'-cGMP 1By similarity | 2 | |
Nucleotide bindingi | 708 – 711 | 3',5'-cGMP 2By similarity | 4 | |
Nucleotide bindingi | 718 – 719 | 3',5'-cGMP 2By similarity | 2 | |
Nucleotide bindingi | 783 – 791 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cGMP binding Source: UniProtKB-KW
- cGMP-dependent protein kinase activity Source: FlyBase
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- feeding behavior Source: FlyBase
- habituation Source: FlyBase
- larval feeding behavior Source: UniProtKB
- larval locomotory behavior Source: FlyBase
- long-term memory Source: FlyBase
- motor neuron axon guidance Source: FlyBase
- protein phosphorylation Source: UniProtKB
- regulation of heart contraction Source: FlyBase
- regulation of response to food Source: FlyBase
- response to sucrose Source: FlyBase
- short-term memory Source: FlyBase
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cGMP, cGMP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.12, 1994 |
Reactomei | R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-DME-392517, Rap1 signalling R-DME-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B (EC:2.7.11.12)Short name: cGK Alternative name(s): Foraging protein |
Gene namesi | Name:for Synonyms:DG2, PGK2, Pkg24A ORF Names:CG10033 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000721, for |
VEuPathDBi | VectorBase:FBgn0000721 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086121 | 1 – 1088 | cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3BAdd BLAST | 1088 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 934 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 938 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q03043 |
PRIDEi | Q03043 |
PTM databases
iPTMneti | Q03043 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0000721, Expressed in spermathecum and 70 other tissues |
ExpressionAtlasi | Q03043, baseline and differential |
Genevisiblei | Q03043, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 69303, 13 interactors |
STRINGi | 7227.FBpp0088350 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 777 – 1036 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 260 | |
Domaini | 1037 – 1088 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 52 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 69 | DimerizationBy similarityAdd BLAST | 69 | |
Regioni | 15 – 39 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 70 – 528 | RegulatoryBy similarityAdd BLAST | 459 | |
Regioni | 128 – 158 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 191 – 211 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 305 – 336 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 441 – 477 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 1064 – 1088 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 29 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 141 – 158 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 454 – 477 | Polar residuesSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0614, Eukaryota KOG0616, Eukaryota |
HOGENOMi | CLU_000288_73_6_1 |
InParanoidi | Q03043 |
PhylomeDBi | Q03043 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR031831, PKcGMP_CC IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF16808, PKcGMP_CC, 1 hit PF00069, Pkinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRFCFDRLCF ATKRPAQNSN SNAPHSSTTV DAPPRPADVD VATVPVATPA
60 70 80 90 100
PPPQQPVSNL FYADYQKLQP AIIDRDWERD RDTDTDTRSE AKPPDIVEHI
110 120 130 140 150
EPVEEQRQIH TQIQSPAEIQ IQIPPTPPAP SIQIQIQQRY RRHSSAEDRN
160 170 180 190 200
LNTRRNDSNI TEALRKAASM QQEPNANYQF PTDLGLVSIV NNNNNTNTHP
210 220 230 240 250
SGSNSGSNNN SNINNNLVGG IVTLPAAGGL IGLEHTASGL RLIPAPPTHS
260 270 280 290 300
DVLTHTLIYG TPPSGAQQLN QDPRSLLHQQ ELQLQQRYQQ LQQLQAQTQG
310 320 330 340 350
LYTSQGSPVL YHQPSPGSSQ PVAIPGATCH SPTQLQPPNT LNLQQQMQSL
360 370 380 390 400
RISGCTPSGT GGSATPSPVG LVDPNFIVSN YVAASPQEER FIQIIQAKEL
410 420 430 440 450
KIQEMQRALQ FKDNEIAELK SHLDKFQSVF PFSRGSAAGC AGTGGASGSG
460 470 480 490 500
AGGSGGSGPG TATGATRKSG QNFQRQRALG ISAEPQSESS LLLEHVSFPK
510 520 530 540 550
YDKDERSREL IKAAILDNDF MKNLDLTQIR EIVDCMYPVK YPAKNLIIKE
560 570 580 590 600
GDVGSIVYVM EDGRVEVSRE GKYLSTLSGA KVLGELAILY NCQRTATITA
610 620 630 640 650
ITECNLWAIE RQCFQTIMMR TGLIRQAEYS DFLKSVPIFK DLAEDTLIKI
660 670 680 690 700
SDVLEETHYQ RGDYIVRQGA RGDTFFIISK GKVRVTIKQQ DTQEEKFIRM
710 720 730 740 750
LGKGDFFGEK ALQGDDLRTA NIICESADGV SCLVIDRETF NQLISNLDEI
760 770 780 790 800
KHRYDDEGAM ERRKINEEFR DINLTDLRVI ATLGVGGFGR VELVQTNGDS
810 820 830 840 850
SRSFALKQMK KSQIVETRQQ QHIMSEKEIM GEANCQFIVK LFKTFKDKKY
860 870 880 890 900
LYMLMESCLG GELWTILRDK GNFDDSTTRF YTACVVEAFD YLHSRNIIYR
910 920 930 940 950
DLKPENLLLN ERGYVKLVDF GFAKKLQTGR KTWTFCGTPE YVAPEVILNR
960 970 980 990 1000
GHDISADYWS LGVLMFELLT GTPPFTGSDP MRTYNIILKG IDAIEFPRNI
1010 1020 1030 1040 1050
TRNASNLIKK LCRDNPAERL GYQRGGISEI QKHKWFDGFY WWGLQNCTLE
1060 1070 1080
PPIKPAVKSV VDTTNFDDYP PDPEGPPPDD VTGWDKDF
The sequence of this isoform differs from the canonical sequence as follows:
1-520: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-169: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-346: Missing.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W5Q0S1 | A0A1W5Q0S1_DROME | cGMP-dependent protein kinase | for 142251_at, anon-WO0140519.260, anon-WO02059370.47, BcDNA:GM08338, DG2 | 742 | Annotation score: | ||
E1JHR9 | E1JHR9_DROME | cGMP-dependent protein kinase | for 142251_at, anon-WO0140519.260, anon-WO02059370.47, BcDNA:GM08338, DG2 | 894 | Annotation score: | ||
A0A4D6K344 | A0A4D6K344_DROME | cGMP-dependent protein kinase | for 142251_at, anon-WO0140519.260, anon-WO02059370.47, BcDNA:GM08338, DG2 | 471 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 49 | P → T in AAA28455 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 207 | S → T in AAA28455 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 664 | Y → H in AAA28455 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 664 | Y → H in AAA28459 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 692 | T → R in AAA28455 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 692 | T → R in AAA28459 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 915 | V → G in AAA28455 (PubMed:2732245).Curated | 1 | |
Sequence conflicti | 915 | V → G in AAA28459 (PubMed:2732245).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004763 | 1 – 520 | Missing in isoform cD4. CuratedAdd BLAST | 520 | |
Alternative sequenceiVSP_004762 | 1 – 346 | Missing in isoform T3B. 1 PublicationAdd BLAST | 346 | |
Alternative sequenceiVSP_004761 | 1 – 169 | Missing in isoform T3A. CuratedAdd BLAST | 169 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27120 , M27117, M27118, M27119 Genomic DNA Translation: AAA28455.1 M30413 mRNA Translation: AAA28459.1 AE014134 Genomic DNA Translation: AAF51082.2 AE014134 Genomic DNA Translation: AAS64613.1 AE014134 Genomic DNA Translation: AAS64614.1 AY061514 mRNA Translation: AAL29062.1 |
PIRi | B34106 |
RefSeqi | NP_477487.1, NM_058139.4 [Q03043-1] NP_995626.1, NM_205904.2 [Q03043-1] NP_995628.1, NM_205906.2 [Q03043-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0089304; FBpp0088350; FBgn0000721 [Q03043-1] FBtr0089310; FBpp0088922; FBgn0000721 [Q03043-1] FBtr0089312; FBpp0088923; FBgn0000721 [Q03043-1] FBtr0446099; FBpp0402801; FBgn0000721 [Q03043-3] FBtr0473363; FBpp0422967; FBgn0000721 [Q03043-4] FBtr0473364; FBpp0422968; FBgn0000721 [Q03043-4] |
GeneIDi | 44817 |
UCSCi | CG10033-RA, d. melanogaster [Q03043-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27120 , M27117, M27118, M27119 Genomic DNA Translation: AAA28455.1 M30413 mRNA Translation: AAA28459.1 AE014134 Genomic DNA Translation: AAF51082.2 AE014134 Genomic DNA Translation: AAS64613.1 AE014134 Genomic DNA Translation: AAS64614.1 AY061514 mRNA Translation: AAL29062.1 |
PIRi | B34106 |
RefSeqi | NP_477487.1, NM_058139.4 [Q03043-1] NP_995626.1, NM_205904.2 [Q03043-1] NP_995628.1, NM_205906.2 [Q03043-1] |
3D structure databases
AlphaFoldDBi | Q03043 |
SMRi | Q03043 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 69303, 13 interactors |
STRINGi | 7227.FBpp0088350 |
PTM databases
iPTMneti | Q03043 |
Proteomic databases
PaxDbi | Q03043 |
PRIDEi | Q03043 |
Protocols and materials databases
DNASUi | 44817 |
Genome annotation databases
EnsemblMetazoai | FBtr0089304; FBpp0088350; FBgn0000721 [Q03043-1] FBtr0089310; FBpp0088922; FBgn0000721 [Q03043-1] FBtr0089312; FBpp0088923; FBgn0000721 [Q03043-1] FBtr0446099; FBpp0402801; FBgn0000721 [Q03043-3] FBtr0473363; FBpp0422967; FBgn0000721 [Q03043-4] FBtr0473364; FBpp0422968; FBgn0000721 [Q03043-4] |
GeneIDi | 44817 |
UCSCi | CG10033-RA, d. melanogaster [Q03043-1] |
Organism-specific databases
CTDi | 44817 |
FlyBasei | FBgn0000721, for |
VEuPathDBi | VectorBase:FBgn0000721 |
Phylogenomic databases
eggNOGi | KOG0614, Eukaryota KOG0616, Eukaryota |
HOGENOMi | CLU_000288_73_6_1 |
InParanoidi | Q03043 |
PhylomeDBi | Q03043 |
Enzyme and pathway databases
BRENDAi | 2.7.11.12, 1994 |
Reactomei | R-DME-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-DME-392517, Rap1 signalling R-DME-9648002, RAS processing |
Miscellaneous databases
BioGRID-ORCSi | 44817, 0 hits in 3 CRISPR screens |
ChiTaRSi | for, fly |
GenomeRNAii | 44817 |
Gene expression databases
Bgeei | FBgn0000721, Expressed in spermathecum and 70 other tissues |
ExpressionAtlasi | Q03043, baseline and differential |
Genevisiblei | Q03043, DM |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR031831, PKcGMP_CC IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF16808, PKcGMP_CC, 1 hit PF00069, Pkinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KGP24_DROME | |
Accessioni | Q03043Primary (citable) accession number: Q03043 Secondary accession number(s): A4V042 Q9VQT2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | February 1, 2005 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families