UniProtKB - Q03019 (TWINE_DROME)
Protein
Cdc25-like protein phosphatase twine
Gene
twe
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required during meiosis. Regulates the transition from the extended G2 phase to the onset of the first meiotic division.
Catalytic activityi
- EC:3.1.3.48
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 318 | By similarity | 1 |
GO - Molecular functioni
- protein tyrosine phosphatase activity Source: FlyBase
GO - Biological processi
- cell division Source: UniProtKB-KW
- embryonic pattern specification Source: FlyBase
- G2/MI transition of meiotic cell cycle Source: FlyBase
- G2/M transition of mitotic cell cycle Source: GO_Central
- male meiotic nuclear division Source: FlyBase
- meiotic nuclear envelope disassembly Source: FlyBase
- positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
- positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
- protein dephosphorylation Source: FlyBase
- regulation of meiotic cell cycle Source: FlyBase
- regulation of syncytial blastoderm mitotic cell cycle Source: FlyBase
- spermatogenesis Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell cycle, Cell division, Meiosis |
Enzyme and pathway databases
Reactomei | R-DME-156711, Polo-like kinase mediated events R-DME-176187, Activation of ATR in response to replication stress R-DME-5625740, RHO GTPases activate PKNs R-DME-5689880, Ub-specific processing proteases R-DME-69202, Cyclin E associated events during G1/S transition R-DME-69273, Cyclin A/B1/B2 associated events during G2/M transition R-DME-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-DME-69656, Cyclin A:Cdk2-associated events at S phase entry R-DME-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:twe ORF Names:CG4965 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002673, twe |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198659 | 1 – 426 | Cdc25-like protein phosphatase twineAdd BLAST | 426 |
Proteomic databases
PaxDbi | Q03019 |
Expressioni
Tissue specificityi
Expressed in developing male and female germ cells.
Gene expression databases
Bgeei | FBgn0002673, Expressed in embryo and 21 other tissues |
ExpressionAtlasi | Q03019, baseline and differential |
Genevisiblei | Q03019, DM |
Interactioni
Binary interactionsi
Q03019
With | #Exp. | IntAct |
---|---|---|
Cdk1 [P23572] | 4 | EBI-138996,EBI-108689 |
Protein-protein interaction databases
BioGRIDi | 60966, 6 interactors |
DIPi | DIP-23538N |
IntActi | Q03019, 3 interactors |
STRINGi | 7227.FBpp0080412 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 265 – 371 | RhodanesePROSITE-ProRule annotationAdd BLAST | 107 |
Sequence similaritiesi
Belongs to the MPI phosphatase family.Curated
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000166659 |
HOGENOMi | CLU_014464_3_1_1 |
InParanoidi | Q03019 |
OMAi | AKTKSWQ |
OrthoDBi | 1423329at2759 |
PhylomeDBi | Q03019 |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q03019-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASKRLMLDV EEEDDESGAC GQENFDPHDA DMEYQAKRRK SAVQETPLQW
60 70 80 90 100
MLKRHIPAST TVLSPITELS QNMNGARLDG TPKSTQRIPA NRTLNNFNSL
110 120 130 140 150
SSRTLGSFSS SCSSYESGNS LDDEYMDMFE MESAENHNLE LPDDLEVLLS
160 170 180 190 200
GQLKSESNLE EMSNKKGSLR RCLSMYPSEQ PEEAVQEPDQ ETNMPMKKMQ
210 220 230 240 250
RKTLSMNDAE IMRALGDEPE LIGDLSKPCT LPCLATGIRH RDLKTISSDT
260 270 280 290 300
LARLIQGEFD EQLGSQGGYE IIDCRYPYEF LGGHIRGAKN LYTRGQIQEA
310 320 330 340 350
FPTLTSNQEN RRIYVFHCEF SSERGPKLLR YLRSNDRSQH THNYPALDYP
360 370 380 390 400
ELYILHNGYK EFFGLYSQLC QPSQYVPMLA PAHNDEFRYF RAKTKSWQCG
410 420
EGGDSGIGGG GSRGLRKSRS RLLYAE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PBC2 | M9PBC2_DROME | Protein-tyrosine-phosphatase | twe BG:DS02740.1, Cdc25, cdc25, Dmel\CG4965, l(2)35Fh | 426 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 48 | L → M in CAA48783 (PubMed:1286615).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94158 mRNA Translation: AAA28413.1 AE014134 Genomic DNA Translation: AAF53508.1 AY061266 mRNA Translation: AAL28814.1 X69018 mRNA Translation: CAA48783.1 |
PIRi | A41910 |
RefSeqi | NP_001260491.1, NM_001273562.2 NP_001260492.1, NM_001273563.1 NP_001260493.1, NM_001273564.2 NP_476633.1, NM_057285.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0080855; FBpp0080412; FBgn0002673 FBtr0331444; FBpp0303861; FBgn0002673 FBtr0331445; FBpp0303862; FBgn0002673 FBtr0331446; FBpp0303863; FBgn0002673 |
GeneIDi | 34954 |
KEGGi | dme:Dmel_CG4965 |
UCSCi | CG4965-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94158 mRNA Translation: AAA28413.1 AE014134 Genomic DNA Translation: AAF53508.1 AY061266 mRNA Translation: AAL28814.1 X69018 mRNA Translation: CAA48783.1 |
PIRi | A41910 |
RefSeqi | NP_001260491.1, NM_001273562.2 NP_001260492.1, NM_001273563.1 NP_001260493.1, NM_001273564.2 NP_476633.1, NM_057285.4 |
3D structure databases
SMRi | Q03019 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 60966, 6 interactors |
DIPi | DIP-23538N |
IntActi | Q03019, 3 interactors |
STRINGi | 7227.FBpp0080412 |
Proteomic databases
PaxDbi | Q03019 |
Genome annotation databases
EnsemblMetazoai | FBtr0080855; FBpp0080412; FBgn0002673 FBtr0331444; FBpp0303861; FBgn0002673 FBtr0331445; FBpp0303862; FBgn0002673 FBtr0331446; FBpp0303863; FBgn0002673 |
GeneIDi | 34954 |
KEGGi | dme:Dmel_CG4965 |
UCSCi | CG4965-RA, d. melanogaster |
Organism-specific databases
CTDi | 34954 |
FlyBasei | FBgn0002673, twe |
Phylogenomic databases
eggNOGi | KOG3772, Eukaryota |
GeneTreei | ENSGT00940000166659 |
HOGENOMi | CLU_014464_3_1_1 |
InParanoidi | Q03019 |
OMAi | AKTKSWQ |
OrthoDBi | 1423329at2759 |
PhylomeDBi | Q03019 |
Enzyme and pathway databases
Reactomei | R-DME-156711, Polo-like kinase mediated events R-DME-176187, Activation of ATR in response to replication stress R-DME-5625740, RHO GTPases activate PKNs R-DME-5689880, Ub-specific processing proteases R-DME-69202, Cyclin E associated events during G1/S transition R-DME-69273, Cyclin A/B1/B2 associated events during G2/M transition R-DME-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-DME-69656, Cyclin A:Cdk2-associated events at S phase entry R-DME-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex |
Miscellaneous databases
BioGRID-ORCSi | 34954, 0 hits in 3 CRISPR screens |
ChiTaRSi | twe, fly |
GenomeRNAii | 34954 |
PROi | PR:Q03019 |
Gene expression databases
Bgeei | FBgn0002673, Expressed in embryo and 21 other tissues |
ExpressionAtlasi | Q03019, baseline and differential |
Genevisiblei | Q03019, DM |
Family and domain databases
CDDi | cd01530, Cdc25, 1 hit |
Gene3Di | 3.40.250.10, 1 hit |
InterProi | View protein in InterPro IPR000751, MPI_Phosphatase IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf |
Pfami | View protein in Pfam PF00581, Rhodanese, 1 hit |
PRINTSi | PR00716, MPIPHPHTASE |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TWINE_DROME | |
Accessioni | Q03019Primary (citable) accession number: Q03019 Secondary accession number(s): Q9V454 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families