UniProtKB - Q03014 (HHEX_HUMAN)
Protein
Hematopoietically-expressed homeobox protein HHEX
Gene
HHEX
Organism
Homo sapiens (Human)
Status
Functioni
Recognizes the DNA sequence 5'-ATTAA-3' (By similarity). Transcriptional repressor (By similarity). Activator of WNT-mediated transcription in conjunction with CTNNB1 (PubMed:20028982). Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity). May play a role in hematopoietic differentiation (PubMed:8096636).By similarity2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 137 – 196 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding, bending Source: BHF-UCL
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- eukaryotic initiation factor 4E binding Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- repressing transcription factor binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: BHF-UCL
- TBP-class protein binding Source: BHF-UCL
- transcription factor binding Source: BHF-UCL
- transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
GO - Biological processi
- anterior/posterior pattern specification Source: UniProtKB
- B cell differentiation Source: BHF-UCL
- cell differentiation Source: GO_Central
- DNA conformation change Source: BHF-UCL
- mRNA export from nucleus Source: BHF-UCL
- negative regulation of angiogenesis Source: BHF-UCL
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
- negative regulation of transcription, DNA-templated Source: BHF-UCL
- negative regulation of transcription by competitive promoter binding Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- negative regulation of transcription by transcription factor localization Source: BHF-UCL
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
- poly(A)+ mRNA export from nucleus Source: BHF-UCL
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of Wnt signaling pathway Source: UniProtKB
- protein localization to nucleus Source: BHF-UCL
- regulation of leukocyte proliferation Source: BHF-UCL
- response to peptide hormone Source: Ensembl
- response to wounding Source: Ensembl
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation, Wnt signaling pathway |
Enzyme and pathway databases
PathwayCommonsi | Q03014 |
SIGNORi | Q03014 |
Names & Taxonomyi
Protein namesi | Recommended name: Hematopoietically-expressed homeobox protein HHEXShort name: Homeobox protein HEX Alternative name(s): Homeobox protein PRH |
Gene namesi | Name:HHEX Synonyms:HEX, PRH, PRHX |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000152804.10 |
HGNCi | HGNC:4901, HHEX |
MIMi | 604420, gene |
neXtProti | NX_Q03014 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: BHF-UCL
- protein-DNA complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 3087 |
OpenTargetsi | ENSG00000152804 |
PharmGKBi | PA29274 |
Miscellaneous databases
Pharosi | Q03014, Tbio |
Polymorphism and mutation databases
BioMutai | HHEX |
DMDMi | 547658 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049074 | 1 – 270 | Hematopoietically-expressed homeobox protein HHEXAdd BLAST | 270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q03014 |
jPOSTi | Q03014 |
MassIVEi | Q03014 |
MaxQBi | Q03014 |
PaxDbi | Q03014 |
PeptideAtlasi | Q03014 |
PRIDEi | Q03014 |
ProteomicsDBi | 58161 |
PTM databases
iPTMneti | Q03014 |
PhosphoSitePlusi | Q03014 |
Expressioni
Tissue specificityi
Liver and promyelocytic leukemia cell line HL-60.3 Publications
Developmental stagei
Expressed during hematopoiesis.
Gene expression databases
Bgeei | ENSG00000152804, Expressed in secondary oocyte and 193 other tissues |
ExpressionAtlasi | Q03014, baseline and differential |
Genevisiblei | Q03014, HS |
Organism-specific databases
HPAi | ENSG00000152804, Tissue enhanced (thyroid) |
Interactioni
Subunit structurei
Interacts with CD81; the interaction prevents nuclear translocation of HHEX (By similarity).
Interacts (via N-terminus) with SOX13; abolishes the SOX13-mediated inhibition of WNT-mediated transcriptional activity via competitive inhibition of the SOX13-TCF7 complex (PubMed:20028982).
By similarity1 PublicationBinary interactionsi
Hide detailsQ03014
GO - Molecular functioni
- eukaryotic initiation factor 4E binding Source: BHF-UCL
- protein homodimerization activity Source: BHF-UCL
- repressing transcription factor binding Source: BHF-UCL
- TBP-class protein binding Source: BHF-UCL
- transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 109335, 41 interactors |
IntActi | Q03014, 28 interactors |
MINTi | Q03014 |
STRINGi | 9606.ENSP00000282728 |
Miscellaneous databases
RNActi | Q03014, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q03014 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q03014 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 137 | Interaction with SOX131 PublicationAdd BLAST | 137 | |
Regioni | 137 – 270 | Required for WNT signaling induction1 PublicationAdd BLAST | 134 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 133 | Pro-richAdd BLAST | 133 |
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0483, Eukaryota |
GeneTreei | ENSGT00940000161748 |
HOGENOMi | CLU_081944_0_0_1 |
InParanoidi | Q03014 |
OMAi | SVNDYTH |
OrthoDBi | 1596695at2759 |
PhylomeDBi | Q03014 |
TreeFami | TF325047 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q03014-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8VU08 | F8VU08_HUMAN | Hematopoietically-expressed homeobo... | HHEX | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | L → V in AAA02988 (PubMed:8103988).Curated | 1 | |
Sequence conflicti | 171 | A → T in AAH14336 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67235 mRNA Translation: CAA47661.1 L16499 mRNA Translation: AAA02988.1 AK314891 mRNA Translation: BAG37405.1 AL590080 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50087.1 CH471066 Genomic DNA Translation: EAW50088.1 BC014336 mRNA Translation: AAH14336.1 BC015110 mRNA Translation: AAH15110.1 BC050638 mRNA Translation: AAH50638.1 Z21533 mRNA Translation: CAA79730.1 |
CCDSi | CCDS7423.1 |
PIRi | JN0767 |
RefSeqi | NP_002720.1, NM_002729.4 |
Genome annotation databases
Ensembli | ENST00000282728; ENSP00000282728; ENSG00000152804 |
GeneIDi | 3087 |
KEGGi | hsa:3087 |
UCSCi | uc001kid.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67235 mRNA Translation: CAA47661.1 L16499 mRNA Translation: AAA02988.1 AK314891 mRNA Translation: BAG37405.1 AL590080 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50087.1 CH471066 Genomic DNA Translation: EAW50088.1 BC014336 mRNA Translation: AAH14336.1 BC015110 mRNA Translation: AAH15110.1 BC050638 mRNA Translation: AAH50638.1 Z21533 mRNA Translation: CAA79730.1 |
CCDSi | CCDS7423.1 |
PIRi | JN0767 |
RefSeqi | NP_002720.1, NM_002729.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2E1O | NMR | - | A | 138-194 | [»] | |
SMRi | Q03014 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109335, 41 interactors |
IntActi | Q03014, 28 interactors |
MINTi | Q03014 |
STRINGi | 9606.ENSP00000282728 |
PTM databases
iPTMneti | Q03014 |
PhosphoSitePlusi | Q03014 |
Polymorphism and mutation databases
BioMutai | HHEX |
DMDMi | 547658 |
Proteomic databases
EPDi | Q03014 |
jPOSTi | Q03014 |
MassIVEi | Q03014 |
MaxQBi | Q03014 |
PaxDbi | Q03014 |
PeptideAtlasi | Q03014 |
PRIDEi | Q03014 |
ProteomicsDBi | 58161 |
Protocols and materials databases
Antibodypediai | 16484, 484 antibodies |
DNASUi | 3087 |
Genome annotation databases
Ensembli | ENST00000282728; ENSP00000282728; ENSG00000152804 |
GeneIDi | 3087 |
KEGGi | hsa:3087 |
UCSCi | uc001kid.4, human |
Organism-specific databases
CTDi | 3087 |
DisGeNETi | 3087 |
EuPathDBi | HostDB:ENSG00000152804.10 |
GeneCardsi | HHEX |
HGNCi | HGNC:4901, HHEX |
HPAi | ENSG00000152804, Tissue enhanced (thyroid) |
MIMi | 604420, gene |
neXtProti | NX_Q03014 |
OpenTargetsi | ENSG00000152804 |
PharmGKBi | PA29274 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0483, Eukaryota |
GeneTreei | ENSGT00940000161748 |
HOGENOMi | CLU_081944_0_0_1 |
InParanoidi | Q03014 |
OMAi | SVNDYTH |
OrthoDBi | 1596695at2759 |
PhylomeDBi | Q03014 |
TreeFami | TF325047 |
Enzyme and pathway databases
PathwayCommonsi | Q03014 |
SIGNORi | Q03014 |
Miscellaneous databases
BioGRID-ORCSi | 3087, 15 hits in 877 CRISPR screens |
EvolutionaryTracei | Q03014 |
GeneWikii | HHEX |
GenomeRNAii | 3087 |
Pharosi | Q03014, Tbio |
PROi | PR:Q03014 |
RNActi | Q03014, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000152804, Expressed in secondary oocyte and 193 other tissues |
ExpressionAtlasi | Q03014, baseline and differential |
Genevisiblei | Q03014, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HHEX_HUMAN | |
Accessioni | Q03014Primary (citable) accession number: Q03014 Secondary accession number(s): B1AQ17, Q96CE9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM