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Protein

Glutathione S-transferase Mu 4

Gene

GSTM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • glutathione binding Source: BHF-UCL
  • glutathione transferase activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
R-HSA-9026762 Biosynthesis of maresin conjugates in tissue regeneration (MCTR)
SABIO-RKiQ03013

Chemistry databases

SwissLipidsiSLP:000001727

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 4 (EC:2.5.1.18)
Alternative name(s):
GST class-mu 4
GST-Mu2
GSTM4-4
Gene namesi
Name:GSTM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000168765.15
HGNCiHGNC:4636 GSTM4
MIMi138333 gene
neXtProtiNX_Q03013

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2948
OpenTargetsiENSG00000168765
PharmGKBiPA29026

Chemistry databases

ChEMBLiCHEMBL2100
DrugBankiDB03706 1-Hydroxy-2-S-Glutathionyl-3-Para-Nitrophenoxy-Propane
DB03597 Gamma-Glutamyl[S-(2-Iodobenzyl)Cysteinyl]Glycine
DB00143 Glutathione
DB03003 Glutathione Sulfonic Acid
DB04132 S-Hexylglutathione
DB03032 S-Octylglutathione

Polymorphism and mutation databases

BioMutaiGSTM4
DMDMi1170096

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858241 – 218Glutathione S-transferase Mu 4Add BLAST218

Proteomic databases

EPDiQ03013
MaxQBiQ03013
PaxDbiQ03013
PeptideAtlasiQ03013
PRIDEiQ03013
ProteomicsDBi58159
58160 [Q03013-2]

2D gel databases

REPRODUCTION-2DPAGEiIPI00008770

PTM databases

iPTMnetiQ03013
PhosphoSitePlusiQ03013

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues.

Gene expression databases

BgeeiENSG00000168765 Expressed in 190 organ(s), highest expression level in right ovary
CleanExiHS_GSTM4
ExpressionAtlasiQ03013 baseline and differential
GenevisibleiQ03013 HS

Organism-specific databases

HPAiHPA055972
HPA055973

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
GSTM3Q6FGJ93EBI-713363,EBI-10209603

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109203, 14 interactors
IntActiQ03013, 12 interactors
STRINGi9606.ENSP00000358851

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ03013
SMRiQ03013
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03013

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione bindingBy similarity2
Regioni46 – 50Glutathione bindingBy similarity5
Regioni59 – 60Glutathione bindingBy similarity2
Regioni72 – 73Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated

Phylogenomic databases

eggNOGiENOG410IN5J Eukaryota
ENOG4110YU0 LUCA
GeneTreeiENSGT00550000074559
HOGENOMiHOG000115735
HOVERGENiHBG106842
InParanoidiQ03013
KOiK00799
OMAiRITESWA
PhylomeDBiQ03013
TreeFamiTF353040

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR003081 GST_mu
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01267 GSTRNSFRASEM
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q03013-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSMTLGYWDI RGLAHAIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGAHKI TQSNAILCYI ARKHNLCGET EEEKIRVDIL
110 120 130 140 150
ENQAMDVSNQ LARVCYSPDF EKLKPEYLEE LPTMMQHFSQ FLGKRPWFVG
160 170 180 190 200
DKITFVDFLA YDVLDLHRIF EPNCLDAFPN LKDFISRFEG LEKISAYMKS
210
SRFLPKPLYT RVAVWGNK
Length:218
Mass (Da):25,561
Last modified:January 23, 2007 - v3
Checksum:i35E96FA54D566B1E
GO
Isoform 2 (identifier: Q03013-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-195: GLEKIS → VSCGIM
     196-218: Missing.

Show »
Length:195
Mass (Da):22,829
Checksum:i32F3BEF15BB378D3
GO
Isoform 3 (identifier: Q03013-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-120: Missing.

Show »
Length:157
Mass (Da):18,627
Checksum:i095AFD32871D3B4A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6NNT0A6NNT0_HUMAN
Glutathione S-transferase
GSTM4
202Annotation score:
A0A0A0MR85A0A0A0MR85_HUMAN
Glutathione S-transferase Mu 4
GSTM4
218Annotation score:
A0A1W2PNX1A0A1W2PNX1_HUMAN
Glutathione S-transferase Mu 4
GSTM4
11Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4SMT → PMI in CAA48637 (PubMed:8471052).Curated3
Sequence conflicti17I → M in AAA57346 (PubMed:8349586).Curated1
Sequence conflicti37D → G in CAA48637 (PubMed:8471052).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0339792S → P. Corresponds to variant dbSNP:rs3211190Ensembl.1
Natural variantiVAR_033980160A → V. Corresponds to variant dbSNP:rs17838158Ensembl.1
Natural variantiVAR_049487208L → V. Corresponds to variant dbSNP:rs112611763Ensembl.1
Natural variantiVAR_049488209Y → F. Corresponds to variant dbSNP:rs112330158Ensembl.1
Natural variantiVAR_049489211R → K. Corresponds to variant dbSNP:rs200675176Ensembl.1
Natural variantiVAR_049490212V → M1 PublicationCorresponds to variant dbSNP:rs149370166Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04768860 – 120Missing in isoform 3. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_011773190 – 195GLEKIS → VSCGIM in isoform 2. 1 Publication6
Alternative sequenceiVSP_011774196 – 218Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96234 mRNA Translation: AAA57347.1
M96233 Genomic DNA Translation: AAA57346.1
X68677 Genomic DNA Translation: CAA48637.1
M99422 mRNA Translation: AAA58623.1
DQ062813 mRNA Translation: AAY98515.1
CR541869 mRNA Translation: CAG46667.1
AK291880 mRNA Translation: BAF84569.1
AC000031 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56405.1
BC015513 mRNA Translation: AAH15513.1
BC108729 mRNA Translation: AAI08730.1
X56837 Genomic DNA Translation: CAA40167.1
CCDSiCCDS806.1 [Q03013-2]
CCDS807.1 [Q03013-1]
PIRiA47486
S32425
RefSeqiNP_000841.1, NM_000850.4 [Q03013-1]
NP_671489.1, NM_147148.2 [Q03013-2]
UniGeneiHs.348387

Genome annotation databases

EnsembliENST00000326729; ENSP00000316471; ENSG00000168765 [Q03013-2]
ENST00000369836; ENSP00000358851; ENSG00000168765 [Q03013-1]
GeneIDi2948
KEGGihsa:2948
UCSCiuc001dyf.4 human [Q03013-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96234 mRNA Translation: AAA57347.1
M96233 Genomic DNA Translation: AAA57346.1
X68677 Genomic DNA Translation: CAA48637.1
M99422 mRNA Translation: AAA58623.1
DQ062813 mRNA Translation: AAY98515.1
CR541869 mRNA Translation: CAG46667.1
AK291880 mRNA Translation: BAF84569.1
AC000031 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56405.1
BC015513 mRNA Translation: AAH15513.1
BC108729 mRNA Translation: AAI08730.1
X56837 Genomic DNA Translation: CAA40167.1
CCDSiCCDS806.1 [Q03013-2]
CCDS807.1 [Q03013-1]
PIRiA47486
S32425
RefSeqiNP_000841.1, NM_000850.4 [Q03013-1]
NP_671489.1, NM_147148.2 [Q03013-2]
UniGeneiHs.348387

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GTUX-ray3.30A/B/C/D/E/F/G/H2-218[»]
ProteinModelPortaliQ03013
SMRiQ03013
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109203, 14 interactors
IntActiQ03013, 12 interactors
STRINGi9606.ENSP00000358851

Chemistry databases

ChEMBLiCHEMBL2100
DrugBankiDB03706 1-Hydroxy-2-S-Glutathionyl-3-Para-Nitrophenoxy-Propane
DB03597 Gamma-Glutamyl[S-(2-Iodobenzyl)Cysteinyl]Glycine
DB00143 Glutathione
DB03003 Glutathione Sulfonic Acid
DB04132 S-Hexylglutathione
DB03032 S-Octylglutathione
SwissLipidsiSLP:000001727

PTM databases

iPTMnetiQ03013
PhosphoSitePlusiQ03013

Polymorphism and mutation databases

BioMutaiGSTM4
DMDMi1170096

2D gel databases

REPRODUCTION-2DPAGEiIPI00008770

Proteomic databases

EPDiQ03013
MaxQBiQ03013
PaxDbiQ03013
PeptideAtlasiQ03013
PRIDEiQ03013
ProteomicsDBi58159
58160 [Q03013-2]

Protocols and materials databases

DNASUi2948
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326729; ENSP00000316471; ENSG00000168765 [Q03013-2]
ENST00000369836; ENSP00000358851; ENSG00000168765 [Q03013-1]
GeneIDi2948
KEGGihsa:2948
UCSCiuc001dyf.4 human [Q03013-1]

Organism-specific databases

CTDi2948
DisGeNETi2948
EuPathDBiHostDB:ENSG00000168765.15
GeneCardsiGSTM4
HGNCiHGNC:4636 GSTM4
HPAiHPA055972
HPA055973
MIMi138333 gene
neXtProtiNX_Q03013
OpenTargetsiENSG00000168765
PharmGKBiPA29026
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IN5J Eukaryota
ENOG4110YU0 LUCA
GeneTreeiENSGT00550000074559
HOGENOMiHOG000115735
HOVERGENiHBG106842
InParanoidiQ03013
KOiK00799
OMAiRITESWA
PhylomeDBiQ03013
TreeFamiTF353040

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
R-HSA-9026762 Biosynthesis of maresin conjugates in tissue regeneration (MCTR)
SABIO-RKiQ03013

Miscellaneous databases

ChiTaRSiGSTM4 human
EvolutionaryTraceiQ03013
GeneWikiiGSTM4
GenomeRNAii2948
PROiPR:Q03013
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168765 Expressed in 190 organ(s), highest expression level in right ovary
CleanExiHS_GSTM4
ExpressionAtlasiQ03013 baseline and differential
GenevisibleiQ03013 HS

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR003081 GST_mu
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01267 GSTRNSFRASEM
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM4_HUMAN
AccessioniPrimary (citable) accession number: Q03013
Secondary accession number(s): A8K765
, Q05465, Q32NC1, Q4JNT8, Q6FH87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 185 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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