UniProtKB - Q02V45 (TRPA_PSEAB)
Protein
Tryptophan synthase alpha chain
Gene
trpA
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Functioni
The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotation
Catalytic activityi
- (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophanUniRule annotationEC:4.2.1.20UniRule annotation
: L-tryptophan biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotationProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Anthranilate synthase component 1 (trpE)
- Anthranilate phosphoribosyltransferase (trpD)
- N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
- Indole-3-glycerol phosphate synthase (trpC)
- Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | Proton acceptorUniRule annotation | 1 | |
Active sitei | 60 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- tryptophan synthase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis |
Enzyme and pathway databases
BioCyci | PAER208963:G1G74-36-MONOMER |
UniPathwayi | UPA00035;UER00044 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:trpAUniRule annotation Ordered Locus Names:PA14_00440 |
Organismi | Pseudomonas aeruginosa (strain UCBPP-PA14) |
Taxonomic identifieri | 208963 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000018253 | 1 – 268 | Tryptophan synthase alpha chainAdd BLAST | 268 |
Proteomic databases
PRIDEi | Q02V45 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta chains.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Belongs to the TrpA family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_016734_0_4_6 |
OMAi | LVMTYWN |
Family and domain databases
CDDi | cd04724, Tryptophan_synthase_alpha, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00131, Trp_synth_alpha, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011060, RibuloseP-bd_barrel IPR018204, Trp_synthase_alpha_AS IPR002028, Trp_synthase_suA |
Pfami | View protein in Pfam PF00290, Trp_syntA, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00262, trpA, 1 hit |
PROSITEi | View protein in PROSITE PS00167, TRP_SYNTHASE_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02V45-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRLQTRFAQ LKQENRAALV TFVTAGDPDY ASSLEILKGL PAAGADVIEL
60 70 80 90 100
GMPFTDPMAD GPAIQLANIR ALDGGQTLAR TLQMVREFRS GDSETPLVLM
110 120 130 140 150
GYFNPIHHYG VERFIAEAKE VGVDGLIVVD LPPEHNEDLC HPAQAAGLDF
160 170 180 190 200
IRLTTPTTGD QRLPTVLEGS SGFVYYVSVA GVTGANAATL EHVEEAVARL
210 220 230 240 250
RRHTDLPIGI GFGIRSAEHA AAVARLADGV VVGSALIDRI AKARDNAQAV
260
KDVLALCGEL AEGVRNAR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000438 Genomic DNA Translation: ABJ14992.1 |
RefSeqi | WP_003097337.1, NZ_CP034244.1 |
Genome annotation databases
EnsemblBacteriai | ABJ14992; ABJ14992; PA14_00440 |
KEGGi | pau:PA14_00440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000438 Genomic DNA Translation: ABJ14992.1 |
RefSeqi | WP_003097337.1, NZ_CP034244.1 |
3D structure databases
SMRi | Q02V45 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q02V45 |
Genome annotation databases
EnsemblBacteriai | ABJ14992; ABJ14992; PA14_00440 |
KEGGi | pau:PA14_00440 |
Phylogenomic databases
HOGENOMi | CLU_016734_0_4_6 |
OMAi | LVMTYWN |
Enzyme and pathway databases
UniPathwayi | UPA00035;UER00044 |
BioCyci | PAER208963:G1G74-36-MONOMER |
Family and domain databases
CDDi | cd04724, Tryptophan_synthase_alpha, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00131, Trp_synth_alpha, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011060, RibuloseP-bd_barrel IPR018204, Trp_synthase_alpha_AS IPR002028, Trp_synthase_suA |
Pfami | View protein in Pfam PF00290, Trp_syntA, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00262, trpA, 1 hit |
PROSITEi | View protein in PROSITE PS00167, TRP_SYNTHASE_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRPA_PSEAB | |
Accessioni | Q02V45Primary (citable) accession number: Q02V45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | November 14, 2006 | |
Last modified: | December 2, 2020 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families