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Entry version 72 (02 Jun 2021)
Sequence version 1 (14 Nov 2006)
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Protein

CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST

Gene

cas3

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential).

Curated

In this bacteria Y.pestis-subtype CRISPRs do not confer resistance to phage DSM3 or MP22, but instead are required for DMS3-dependent inhibition of biofilm formation and possibly motility.

1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi124MagnesiumSequence analysis1
Metal bindingi220MagnesiumSequence analysis1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase
Biological processAntiviral defense
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
PAER208963:G1G74-2806-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST (EC:3.1.-.-, EC:3.6.4.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:cas3
Ordered Locus Names:PA14_33340
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri208963 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000653 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Infection with phage DSM3 inhibits biofilm formation; disrupting this gene restores biofilm formation upon infection with DMS3 infection. Normal biofilm formation in the absence of phage infection. Decreased production of crRNA in the presence or absence of phage. Disruption of the entire Y.pestis-subtype CRISPR region disrupts crRNA production but does not alter phage resistance (possibly OLNs PA14_33350 to PA14_33310, plus the flanking CRISPR loci), indicating this CRISPR is not involved in phage resistance.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi124D → A in a disruption mutant, does not restore biofilm formation, restores crRNA production. 1 Publication1
Mutagenesisi576D → A in a disruption mutant, does not restore biofilm formation, restores crRNA production. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004176051 – 1076CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPESTAdd BLAST1076

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q02ML8

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with Cas1.

By similarity

Protein-protein interaction databases

Database of interacting proteins

More...
DIPi
DIP-61771N

Protein interaction database and analysis system

More...
IntActi
Q02ML8, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11076
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q02ML8

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini102 – 318HD Cas3-typePROSITE-ProRule annotationAdd BLAST217

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi576 – 579DEAD box4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.Curated
In the central section; belongs to the CRISPR-associated helicase Cas3 family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_009385_0_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
VACHWIR

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.3210.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006483, CRISPR-assoc_Cas3_HD
IPR038257, CRISPR-assoc_Cas3_HD_sf
IPR013395, Helicase_Cas3_CRISPR-ass_yers
IPR027417, P-loop_NTPase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF18019, HD_6, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02562, cas3_yersinia, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51643, HD_CAS3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q02ML8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNILLVSQCE KRALSETRRI LDQFAERRGE RTWQTPITQA GLDTLRRLLK
60 70 80 90 100
KSARRNTAVA CHWIRGRDHS ELLWIVGDAS RFNAQGAVPT NRTCRDILRK
110 120 130 140 150
EDENDWHSAE DIRLLTVMAA LFHDIGKASQ AFQAKLRNRG KPMADAYRHE
160 170 180 190 200
WVSLRLFEAF VGPGSSDEDW LRRLADKRET GDAWLSQLAR DDRQSAPPGP
210 220 230 240 250
FQKSRLPPLA QAVGWLIVSH HRLPNGDHRG SASLARLPAP IQSQWCGARD
260 270 280 290 300
ADAKEKAACW QFPHGLPFAS AHWRARTALC AQSMLERPGL LARGPALLHD
310 320 330 340 350
SYVMHVSRLI LMLADHHYSS LPADSRLGDP NFPLHANTDR DSGKLKQRLD
360 370 380 390 400
EHLLGVALHS RKLAGTLPRL ERQLPRLARH KGFTRRVEQP RFRWQDKAYD
410 420 430 440 450
CAMACREQAM EHGFFGLNLA STGCGKTLAN GRILYALADP QRGARFSIAL
460 470 480 490 500
GLRSLTLQTG QAYRERLGLG DDDLAILVGG SAARELFEKQ QERLERSGSE
510 520 530 540 550
SAQELLAENS HVHFAGTLED GPLREWLGRN SAGNRLLQAP ILACTIDHLM
560 570 580 590 600
PASESLRGGH QIAPLLRLMT SDLVLDEVDD FDIDDLPALS RLVHWAGLFG
610 620 630 640 650
SRVLLSSATL PPALVQGLFE AYRSGREIFQ RHRGAPGRAT EIRCAWFDEF
660 670 680 690 700
SSQSSAHGAV TSFSEAHATF VAQRLAKLEQ LPPRRQAQLC TVHAAGEARP
710 720 730 740 750
ALCRELAGQM NTWMADLHRC HHTEHQGRRI SFGLLRLANI EPLIELAQAI
760 770 780 790 800
LAQGAPEGLH VHLCVYHSRH PLLVRSAIER QLDELLKRSD DDAAALFARP
810 820 830 840 850
TLAKALQAST ERDHLFVVLA SPVAEVGRDH DYDWAIVEPS SMRSIIQLAG
860 870 880 890 900
RIRRHRSGFS GEANLYLLSR NIRSLEGQNP AFQRPGFETP DFPLDSHDLH
910 920 930 940 950
DLLDPALLAR IDASPRIVEP FPLFPRSRLV DLEHRRLRAL MLADDPPSSL
960 970 980 990 1000
LGVPLWWQTP ASLSGALQTS QPFRAGAKER CYALLPDEDD EERLHFSRYE
1010 1020 1030 1040 1050
EGTWSNQDNL LRNLDLTYGP RIQTWGTVNY REELVAMAGR EDLDLRQCAM
1060 1070
RYGEVRLREN TQGWSYHPYL GFKKYN
Length:1,076
Mass (Da):121,091
Last modified:November 14, 2006 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i8C66F4C5F622BB49
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CP000438 Genomic DNA Translation: ABJ11601.1

NCBI Reference Sequences

More...
RefSeqi
WP_003139227.1, NZ_CP034244.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ABJ11601; ABJ11601; PA14_33340

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
pau:PA14_33340

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA Translation: ABJ11601.1
RefSeqiWP_003139227.1, NZ_CP034244.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B7IX-ray2.60A1-1076[»]
5GQHelectron microscopy4.20A1-1076[»]
SMRiQ02ML8
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

DIPiDIP-61771N
IntActiQ02ML8, 1 interactor

Proteomic databases

PRIDEiQ02ML8

Genome annotation databases

EnsemblBacteriaiABJ11601; ABJ11601; PA14_33340
KEGGipau:PA14_33340

Phylogenomic databases

HOGENOMiCLU_009385_0_0_6
OMAiVACHWIR

Enzyme and pathway databases

BioCyciPAER208963:G1G74-2806-MONOMER

Family and domain databases

Gene3Di1.10.3210.30, 1 hit
InterProiView protein in InterPro
IPR006483, CRISPR-assoc_Cas3_HD
IPR038257, CRISPR-assoc_Cas3_HD_sf
IPR013395, Helicase_Cas3_CRISPR-ass_yers
IPR027417, P-loop_NTPase
PfamiView protein in Pfam
PF18019, HD_6, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit
TIGRFAMsiTIGR02562, cas3_yersinia, 1 hit
PROSITEiView protein in PROSITE
PS51643, HD_CAS3, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCAS3_PSEAB
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q02ML8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: November 14, 2006
Last modified: June 2, 2021
This is version 72 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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