UniProtKB - Q02932 (KA120_YEAST)
Protein
Importin beta-like protein KAP120
Gene
KAP120
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, RAN-dependent mechanism. Required for nuclear import of Ho endonuclease and RFP1, and involved in rRNA-processing and assembly or export of 60S ribosomal subunits.4 Publications
Miscellaneous
Present with 6300 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- nuclear import signal receptor activity Source: SGD
- nuclear localization sequence binding Source: SGD
- Ran GTPase binding Source: InterPro
GO - Biological processi
- protein import into nucleus Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Importin beta-like protein KAP120Alternative name(s): Karyopherin-120 |
Gene namesi | Name:KAP120 Ordered Locus Names:YPL125W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006046, KAP120 |
VEuPathDBi | FungiDB:YPL125W |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nuclear envelope Source: GO_Central
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000255954 | 2 – 1032 | Importin beta-like protein KAP120Add BLAST | 1031 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q02932 |
PaxDbi | Q02932 |
PRIDEi | Q02932 |
PTM databases
iPTMneti | Q02932 |
Interactioni
Subunit structurei
Interacts with GTP-bound GSP1 and RFP1. Associates with the nuclear pore complex.
1 PublicationGO - Molecular functioni
- Ran GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 36056, 163 interactors |
DIPi | DIP-4000N |
IntActi | Q02932, 12 interactors |
STRINGi | 4932.YPL125W |
Miscellaneous databases
RNActi | Q02932, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02932 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 103 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 73 |
Sequence similaritiesi
Belongs to the importin beta family.Curated
Phylogenomic databases
eggNOGi | KOG1993, Eukaryota |
GeneTreei | ENSGT00390000014071 |
HOGENOMi | CLU_003886_0_0_1 |
InParanoidi | Q02932 |
OMAi | WLLEEWF |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q02932-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASSLNELNL VQVLEQASNP QHIRSDVQKL AEQQLRQWET QAGFHYLLQS
60 70 80 90 100
IYLNLSNSLQ IRWLAVIQFK NGVDKYWRST RINAIPKDEK ASIRGRLFEM
110 120 130 140 150
IDEQNNQLCI QNAQASARIA RLDFPVEWPT LFEDLENLLN DEIIRKDSVK
160 170 180 190 200
IYNILMHINQ IVKVLGTARI GRCRPAMQSK VPLILPLIVR IYLQSFEEWT
210 220 230 240 250
TSSNLNYEDL SSLQVSYLAL KVLRRIICEG YDRPQTDQSV CDFIKLSVSH
260 270 280 290 300
FEMLISNHEN FKKFDIYEKF IKCLGKLYFN LVTGSPANFI LLPCSTQILI
310 320 330 340 350
TYTRLIFDKA PKVYRENSDV TGDFWEQTAI RGLLILKRVI NFIHKKGAIT
360 370 380 390 400
LKARSDKLTI DASINKINTE FLNENLITRL VDTLMEWYLR LRPTELENWF
410 420 430 440 450
MDPEEWINEQ MATSYEYQIR PCAENVFQDL MNTFSELLVP YLLKKIENDA
460 470 480 490 500
SKLSNSLDDF LRKDAIYASF QLSASAVSEM VDFDRLLIQV FLPEATNTNI
510 520 530 540 550
SGDELRIIRR RVALIINEWS TVKCSEESKS LCYKLFTNFL TDEDDKVVLL
560 570 580 590 600
TTVQTVRTMV DDWNFNKDTF QPFLTENVHL LLRKILPSVS LTETRLYVLN
610 620 630 640 650
TLSDIIIQTK PLISRDLLVE ILQIIPNLWE IATNNASEAI LANALLRLLR
660 670 680 690 700
NLVSSLGSQS HLTWDIAIPV VALACDPSSM QYQLLSEDGY ELWGMLLQNF
710 720 730 740 750
SSHDQEFDDK FVELVPFLKY GIETHTEILP TLLEIIKSYA LILNPVDFFS
760 770 780 790 800
NNTFQDIFKQ MSKYLLKLRE DSFQLVLEIW EILILSNESD YENLLLQKFY
810 820 830 840 850
ETGVLSALFD AIFLEEAPSS YLCSQIIQII ARISYVNPDA LMTFLATYHD
860 870 880 890 900
NLPTSNENAR MPESIRKIVS KDQTYDSVVN KLLTGWIVCF RDIFDPKFKK
910 920 930 940 950
VHILGISSLL RTGLVPILTE FSSIASLWIE MLEEINETNR GDCEKYHLND
960 970 980 990 1000
IVTEQSIAFH PLTAEQLRYH QLCKNNDPVH NISLKDFISQ SMEYLESHLG
1010 1020 1030
VERYQEFLKT INPSLLENLQ MFLSIQPQEA RP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43503 Genomic DNA Translation: AAB68237.1 BK006949 Genomic DNA Translation: DAA11308.1 |
PIRi | S61997 |
RefSeqi | NP_015200.1, NM_001183939.1 |
Genome annotation databases
EnsemblFungii | YPL125W_mRNA; YPL125W; YPL125W |
GeneIDi | 855978 |
KEGGi | sce:YPL125W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43503 Genomic DNA Translation: AAB68237.1 BK006949 Genomic DNA Translation: DAA11308.1 |
PIRi | S61997 |
RefSeqi | NP_015200.1, NM_001183939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6FVB | X-ray | 3.30 | A | 2-1032 | [»] | |
SMRi | Q02932 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36056, 163 interactors |
DIPi | DIP-4000N |
IntActi | Q02932, 12 interactors |
STRINGi | 4932.YPL125W |
PTM databases
iPTMneti | Q02932 |
Proteomic databases
MaxQBi | Q02932 |
PaxDbi | Q02932 |
PRIDEi | Q02932 |
Genome annotation databases
EnsemblFungii | YPL125W_mRNA; YPL125W; YPL125W |
GeneIDi | 855978 |
KEGGi | sce:YPL125W |
Organism-specific databases
SGDi | S000006046, KAP120 |
VEuPathDBi | FungiDB:YPL125W |
Phylogenomic databases
eggNOGi | KOG1993, Eukaryota |
GeneTreei | ENSGT00390000014071 |
HOGENOMi | CLU_003886_0_0_1 |
InParanoidi | Q02932 |
OMAi | WLLEEWF |
Miscellaneous databases
PROi | PR:Q02932 |
RNActi | Q02932, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KA120_YEAST | |
Accessioni | Q02932Primary (citable) accession number: Q02932 Secondary accession number(s): D6W3P2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names