UniProtKB - Q02834 (NANH_MICVI)
Protein
Sialidase
Gene
nedA
Organism
Micromonospora viridifaciens
Status
Functioni
To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.
Catalytic activityi
- Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. EC:3.2.1.18
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 68 | SubstrateBy similarity | 1 | |
Active sitei | 92 | Proton acceptorBy similarity | 1 | |
Active sitei | 260 | NucleophileSequence analysis | 1 | |
Binding sitei | 276 | SubstrateBy similarity | 1 | |
Active sitei | 370 | Nucleophile | 1 |
GO - Molecular functioni
- exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
- exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
- exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
GO - Biological processi
- metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BRENDAi | 3.2.1.18, 7474 |
SABIO-RKi | Q02834 |
Protein family/group databases
CAZyi | CBM32, Carbohydrate-Binding Module Family 32 GH33, Glycoside Hydrolase Family 33 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nedA |
Organismi | Micromonospora viridifaciens |
Taxonomic identifieri | 1881 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Micromonosporales › Micromonosporaceae › Micromonospora |
Subcellular locationi
GO - Cellular componenti
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Chemistry databases
DrugBanki | DB03991, 2-deoxy-2,3-dehydro-N-acetylneuraminic acid DB04465, Lactose DB04265, N-acetyl-beta-neuraminic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 37 | 1 PublicationAdd BLAST | 37 | |
ChainiPRO_0000012032 | 38 – 647 | SialidaseAdd BLAST | 610 |
Proteomic databases
PRIDEi | Q02834 |
Expressioni
Inductioni
By N-acetylneuraminic acid, colominic acid, and sialic acid.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02834 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02834 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 102 – 113 | BNR 1Add BLAST | 12 | |
Repeati | 175 – 186 | BNR 2Add BLAST | 12 | |
Repeati | 239 – 250 | BNR 3Add BLAST | 12 | |
Repeati | 287 – 298 | BNR 4Add BLAST | 12 | |
Repeati | 348 – 359 | BNR 5Add BLAST | 12 | |
Domaini | 496 – 646 | F5/8 type CPROSITE-ProRule annotationAdd BLAST | 151 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 33 family.Curated
Keywords - Domaini
Repeat, SignalFamily and domain databases
CDDi | cd00057, FA58C, 1 hit |
Gene3Di | 2.60.120.260, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR018905, A-galactase_NEW3 IPR000421, FA58C IPR008979, Galactose-bd-like_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR011040, Sialidase IPR026856, Sialidase_fam IPR036278, Sialidase_sf IPR006311, TAT_signal |
PANTHERi | PTHR10628, PTHR10628, 1 hit |
Pfami | View protein in Pfam PF13088, BNR_2, 1 hit PF00754, F5_F8_type_C, 1 hit PF10633, NPCBM_assoc, 1 hit |
SMARTi | View protein in SMART SM00231, FA58C, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF50939, SSF50939, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50022, FA58C_3, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q02834-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTANPYLRRL PRRRAVSFLL APALAAATVA GASPAQAIAG APVPPGGEPL
60 70 80 90 100
YTEQDLAVNG REGFPNYRIP ALTVTPDGDL LASYDGRPTG IDAPGPNSIL
110 120 130 140 150
QRRSTDGGRT WGEQQVVSAG QTTAPIKGFS DPSYLVDRET GTIFNFHVYS
160 170 180 190 200
QRQGFAGSRP GTDPADPNVL HANVATSTDG GLTWSHRTIT ADITPDPGWR
210 220 230 240 250
SRFAASGEGI QLRYGPHAGR LIQQYTIINA AGAFQAVSVY SDDHGRTWRA
260 270 280 290 300
GEAVGVGMDE NKTVELSDGR VLLNSRDSAR SGYRKVAVST DGGHSYGPVT
310 320 330 340 350
IDRDLPDPTN NASIIRAFPD APAGSARAKV LLFSNAASQT SRSQGTIRMS
360 370 380 390 400
CDDGQTWPVS KVFQPGSMSY STLTALPDGT YGLLYEPGTG IRYANFNLAW
410 420 430 440 450
LGGICAPFTI PDVALEPGQQ VTVPVAVTNQ SGIAVPKPSL QLDASPDWQV
460 470 480 490 500
QGSVEPLMPG RQAKGQVTIT VPAGTTPGRY RVGATLRTSA GNASTTFTVT
510 520 530 540 550
VGLLDQARMS IADVDSEETA REDGRASNVI DGNPSTFWHT EWSRADAPGY
560 570 580 590 600
PHRISLDLGG THTISGLQYT RRQNSANEQV ADYEIYTSLN GTTWDGPVAS
610 620 630 640
GRFTTSLAPQ RAVFPARDAR YIRLVALSEQ TGHKYAAVAE LEVEGQR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D01045 Genomic DNA Translation: BAA00852.1 |
PIRi | A45244 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D01045 Genomic DNA Translation: BAA00852.1 |
PIRi | A45244 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EUR | X-ray | 1.82 | A | 43-407 | [»] | |
1EUS | X-ray | 2.00 | A | 43-407 | [»] | |
1EUT | X-ray | 2.50 | A | 43-647 | [»] | |
1EUU | X-ray | 2.50 | A | 43-647 | [»] | |
1W8N | X-ray | 2.10 | A | 47-647 | [»] | |
1W8O | X-ray | 1.70 | A | 47-647 | [»] | |
1WCQ | X-ray | 2.10 | A/B/C | 47-647 | [»] | |
2BER | X-ray | 1.80 | A | 47-647 | [»] | |
2BZD | X-ray | 2.00 | A/B/C | 47-647 | [»] | |
4J9T | X-ray | 1.40 | A | 47-407 | [»] | |
SMRi | Q02834 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB03991, 2-deoxy-2,3-dehydro-N-acetylneuraminic acid DB04465, Lactose DB04265, N-acetyl-beta-neuraminic acid |
Protein family/group databases
CAZyi | CBM32, Carbohydrate-Binding Module Family 32 GH33, Glycoside Hydrolase Family 33 |
Proteomic databases
PRIDEi | Q02834 |
Enzyme and pathway databases
BRENDAi | 3.2.1.18, 7474 |
SABIO-RKi | Q02834 |
Miscellaneous databases
EvolutionaryTracei | Q02834 |
Family and domain databases
CDDi | cd00057, FA58C, 1 hit |
Gene3Di | 2.60.120.260, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR018905, A-galactase_NEW3 IPR000421, FA58C IPR008979, Galactose-bd-like_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR011040, Sialidase IPR026856, Sialidase_fam IPR036278, Sialidase_sf IPR006311, TAT_signal |
PANTHERi | PTHR10628, PTHR10628, 1 hit |
Pfami | View protein in Pfam PF13088, BNR_2, 1 hit PF00754, F5_F8_type_C, 1 hit PF10633, NPCBM_assoc, 1 hit |
SMARTi | View protein in SMART SM00231, FA58C, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF50939, SSF50939, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50022, FA58C_3, 1 hit PS51318, TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NANH_MICVI | |
Accessioni | Q02834Primary (citable) accession number: Q02834 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries