UniProtKB - Q02825 (SEN54_YEAST)
Protein
tRNA-splicing endonuclease subunit SEN54
Gene
SEN54
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. May be required to embody the molecular ruler of the complex.
Miscellaneous
The tRNA splicing endonuclease complex is present with 100 molecules/cell.1 Publication
Present with 531 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
- tRNA-type intron splice site recognition and cleavage Source: SGD
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-33989-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA-splicing endonuclease subunit SEN54Alternative name(s): Splicing endonuclease of 54 kDa tRNA-intron endonuclease SEN54 |
Gene namesi | Name:SEN54 Ordered Locus Names:YPL083C ORF Names:LPF3C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006004, SEN54 |
VEuPathDBi | FungiDB:YPL083C |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side 2 Publications
Nucleus
- Nucleus 1 Publication
Other locations
- Endomembrane system 1 Publication; Peripheral membrane protein 1 Publication
Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.
Mitochondrion
- mitochondrial outer membrane Source: SGD
- mitochondrion Source: SGD
Nucleus
- tRNA-intron endonuclease complex Source: SGD
Other locations
- endomembrane system Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194034 | 1 – 467 | tRNA-splicing endonuclease subunit SEN54Add BLAST | 467 |
Proteomic databases
MaxQBi | Q02825 |
PaxDbi | Q02825 |
PRIDEi | Q02825 |
Interactioni
Subunit structurei
tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54.
Interacts directly with SEN2.
1 PublicationBinary interactionsi
Q02825
With | #Exp. | IntAct |
---|---|---|
SEN2 [P16658] | 4 | EBI-16829,EBI-16953 |
Protein-protein interaction databases
BioGRIDi | 36098, 111 interactors |
ComplexPortali | CPX-1832, tRNA-intron endonuclease complex |
DIPi | DIP-4452N |
IntActi | Q02825, 5 interactors |
MINTi | Q02825 |
STRINGi | 4932.YPL083C |
Miscellaneous databases
RNActi | Q02825, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 19 – 27 | Poly-Glu | 9 | |
Compositional biasi | 353 – 356 | Poly-Asp | 4 |
Sequence similaritiesi
Belongs to the SEN54 family.Curated
Phylogenomic databases
eggNOGi | KOG4772, Eukaryota |
GeneTreei | ENSGT00390000004214 |
HOGENOMi | CLU_028449_0_1_1 |
InParanoidi | Q02825 |
OMAi | RLGYIIQ |
Family and domain databases
InterProi | View protein in InterPro IPR024337, tRNA_splic_suSen54 IPR024336, tRNA_splic_suSen54_N |
PANTHERi | PTHR21027, PTHR21027, 1 hit |
Pfami | View protein in Pfam PF12928, tRNA_int_end_N2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02825-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQFAGKKTDQ VTTSNPGFEE EEEEEEELQQ DWSQLASLVS KNAALSLPKR
60 70 80 90 100
GEKDYEPDGT NLQDLLLYNA SKAMFDTISD SIRGTTVKSE VRGYYVPHKH
110 120 130 140 150
QAVLLKPKGS FMQTMGRADS TGELWLDFHE FVYLAERGTI LPYYRLEAGS
160 170 180 190 200
NKSSKHETEI LLSMEDLYSL FSSQQEMDQY FVFAHLKRLG FILKPSNQEA
210 220 230 240 250
AVKTSFFPLK KQRSNLQAIT WRLLSLFKIQ ELSLFSGFFY SKWNFFFRKY
260 270 280 290 300
TTSPQLYQGL NRLVRSVAVP KNKKELLDAQ SDREFQKVKD IPLTFKVWKP
310 320 330 340 350
HSNFKKRDPG LPDFQVFVYN KNDDLQHFPT YKELRSMFSS LDYKFEFLSE
360 370 380 390 400
IEDDDDWETN SYVEDIPRKE YIHKRSAKSQ TEKSESSMKA SFQKKTAQSS
410 420 430 440 450
TKKKRKAYPP HIQQNRRLKT GYRSFIIAIM DNGLISFVKM SEADFGSESV
460
WYTPNTQKKV DQRWKKH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41849 Genomic DNA Translation: AAB68256.1 BK006949 Genomic DNA Translation: DAA11350.1 |
PIRi | S61105 |
RefSeqi | NP_015242.1, NM_001183897.1 |
Genome annotation databases
EnsemblFungii | YPL083C_mRNA; YPL083C; YPL083C |
GeneIDi | 856022 |
KEGGi | sce:YPL083C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41849 Genomic DNA Translation: AAB68256.1 BK006949 Genomic DNA Translation: DAA11350.1 |
PIRi | S61105 |
RefSeqi | NP_015242.1, NM_001183897.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36098, 111 interactors |
ComplexPortali | CPX-1832, tRNA-intron endonuclease complex |
DIPi | DIP-4452N |
IntActi | Q02825, 5 interactors |
MINTi | Q02825 |
STRINGi | 4932.YPL083C |
Proteomic databases
MaxQBi | Q02825 |
PaxDbi | Q02825 |
PRIDEi | Q02825 |
Genome annotation databases
EnsemblFungii | YPL083C_mRNA; YPL083C; YPL083C |
GeneIDi | 856022 |
KEGGi | sce:YPL083C |
Organism-specific databases
SGDi | S000006004, SEN54 |
VEuPathDBi | FungiDB:YPL083C |
Phylogenomic databases
eggNOGi | KOG4772, Eukaryota |
GeneTreei | ENSGT00390000004214 |
HOGENOMi | CLU_028449_0_1_1 |
InParanoidi | Q02825 |
OMAi | RLGYIIQ |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-33989-MONOMER |
Miscellaneous databases
PROi | PR:Q02825 |
RNActi | Q02825, protein |
Family and domain databases
InterProi | View protein in InterPro IPR024337, tRNA_splic_suSen54 IPR024336, tRNA_splic_suSen54_N |
PANTHERi | PTHR21027, PTHR21027, 1 hit |
Pfami | View protein in Pfam PF12928, tRNA_int_end_N2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SEN54_YEAST | |
Accessioni | Q02825Primary (citable) accession number: Q02825 Secondary accession number(s): D6W3T4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families