UniProtKB - Q02821 (IMA1_YEAST)
Protein
Importin subunit alpha
Gene
SRP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).By similarity3 Publications
Miscellaneous
Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the karyopherin heterodimer by binding to the beta subunit and releases both subunits from the docking site.
Present with 2790 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- disordered domain specific binding Source: CAFA
- nuclear import signal receptor activity Source: SGD
- nuclear localization sequence binding Source: GO_Central
- protein-containing complex binding Source: SGD
GO - Biological processi
- NLS-bearing protein import into nucleus Source: SGD
- proteasome localization Source: SGD
- protein targeting to membrane Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit alphaAlternative name(s): Karyopherin subunit alpha Karyopherin-60 Serine-rich RNA polymerase I suppressor protein |
Gene namesi | Name:SRP1 Synonyms:KAP60 Ordered Locus Names:YNL189W ORF Names:N1606 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL189W |
SGDi | S000005133, SRP1 |
Subcellular locationi
Other locations
Note: Mainly localized at the periphery of the nucleus.
Nucleus
- nucleoplasm Source: GO_Central
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- NLS-dependent protein nuclear import complex Source: SGD
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 116 | S → F in SRP1-31; temperature-sensitive mutant; reduced growth rate and chromosome loss. 1 Publication | 1 | |
Mutagenesisi | 145 | E → K in SRP1-49; temperature-sensitive mutant; alteration in nucleolar and microtubule morphology. 1 Publication | 1 | |
Mutagenesisi | 219 | P → Q in SRP1-1; temperature-sensitive mutant. 1 Publication | 1 | |
Mutagenesisi | 286 | D → N in SRP1-3; temperature-sensitive mutant. 1 Publication | 1 | |
Mutagenesisi | 360 | E → K in SRP1-2; temperature-sensitive mutant. 1 Publication | 1 | |
Mutagenesisi | 459 | G → V in SRP1-54; temperature-sensitive mutant; reduced growth rate. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120744 | 1 – 542 | Importin subunit alphaAdd BLAST | 542 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q02821 |
PaxDbi | Q02821 |
PRIDEi | Q02821 |
PTM databases
iPTMneti | Q02821 |
Interactioni
Subunit structurei
Forms a complex with an importin beta subunit. In the nucleus, interacts with NUP2 which accelerate release of NLSs, NUP2 is subsequently displaced by CSE1:RanGTP which mediates re-export and recycling.
Interacts with HEH2, SHE2, and STS1.
7 PublicationsBinary interactionsi
Hide detailsQ02821
GO - Molecular functioni
- disordered domain specific binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 35644, 422 interactors |
ComplexPortali | CPX-1068, Importin complex, KAP60-KAP95 |
DIPi | DIP-728N |
ELMi | Q02821 |
IntActi | Q02821, 142 interactors |
MINTi | Q02821 |
STRINGi | 4932.YNL189W |
Miscellaneous databases
RNActi | Q02821, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02821 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02821 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 65 | IBBPROSITE-ProRule annotationAdd BLAST | 65 | |
Repeati | 89 – 122 | ARM 1; truncatedPROSITE-ProRule annotation1 PublicationAdd BLAST | 34 | |
Repeati | 123 – 162 | ARM 2PROSITE-ProRule annotation1 PublicationAdd BLAST | 40 | |
Repeati | 163 – 204 | ARM 3PROSITE-ProRule annotation1 PublicationAdd BLAST | 42 | |
Repeati | 205 – 251 | ARM 4PROSITE-ProRule annotation1 PublicationAdd BLAST | 47 | |
Repeati | 252 – 288 | ARM 5PROSITE-ProRule annotation1 PublicationAdd BLAST | 37 | |
Repeati | 289 – 330 | ARM 6PROSITE-ProRule annotation1 PublicationAdd BLAST | 42 | |
Repeati | 331 – 372 | ARM 7PROSITE-ProRule annotation1 PublicationAdd BLAST | 42 | |
Repeati | 373 – 417 | ARM 8PROSITE-ProRule annotation1 PublicationAdd BLAST | 45 | |
Repeati | 418 – 471 | ARM 9PROSITE-ProRule annotation1 PublicationAdd BLAST | 54 | |
Repeati | 472 – 508 | ARM 10; atypicalPROSITE-ProRule annotation1 PublicationAdd BLAST | 37 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 209 – 335 | NLS binding site 1Add BLAST | 127 | |
Regioni | 419 – 505 | NLS binding site 2Add BLAST | 87 |
Domaini
The NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
Sequence similaritiesi
Belongs to the importin alpha family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01000000214366 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | Q02821 |
OMAi | GEQEANQ |
Family and domain databases
DisProti | DP02227 |
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
IDEALi | IID50012 |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 2 hits PS51214, IBB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02821-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNGTDSSTS KFVPEYRRTN FKNKGRFSAD ELRRRRDTQQ VELRKAKRDE
60 70 80 90 100
ALAKRRNFIP PTDGADSDEE DESSVSADQQ FYSQLQQELP QMTQQLNSDD
110 120 130 140 150
MQEQLSATVK FRQILSREHR PPIDVVIQAG VVPRLVEFMR ENQPEMLQLE
160 170 180 190 200
AAWALTNIAS GTSAQTKVVV DADAVPLFIQ LLYTGSVEVK EQAIWALGNV
210 220 230 240 250
AGDSTDYRDY VLQCNAMEPI LGLFNSNKPS LIRTATWTLS NLCRGKKPQP
260 270 280 290 300
DWSVVSQALP TLAKLIYSMD TETLVDACWA ISYLSDGPQE AIQAVIDVRI
310 320 330 340 350
PKRLVELLSH ESTLVQTPAL RAVGNIVTGN DLQTQVVINA GVLPALRLLL
360 370 380 390 400
SSPKENIKKE ACWTISNITA GNTEQIQAVI DANLIPPLVK LLEVAEYKTK
410 420 430 440 450
KEACWAISNA SSGGLQRPDI IRYLVSQGCI KPLCDLLEIA DNRIIEVTLD
460 470 480 490 500
ALENILKMGE ADKEARGLNI NENADFIEKA GGMEKIFNCQ QNENDKIYEK
510 520 530 540
AYKIIETYFG EEEDAVDETM APQNAGNTFG FGSNVNQQFN FN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75849 Genomic DNA Translation: AAA35090.1 Z71465 Genomic DNA Translation: CAA96083.1 BK006947 Genomic DNA Translation: DAA10364.1 |
PIRi | S30884 |
RefSeqi | NP_014210.1, NM_001183027.1 |
Genome annotation databases
EnsemblFungii | YNL189W_mRNA; YNL189W; YNL189W |
GeneIDi | 855532 |
KEGGi | sce:YNL189W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75849 Genomic DNA Translation: AAA35090.1 Z71465 Genomic DNA Translation: CAA96083.1 BK006947 Genomic DNA Translation: DAA10364.1 |
PIRi | S30884 |
RefSeqi | NP_014210.1, NM_001183027.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BK5 | X-ray | 2.20 | A/B | 89-510 | [»] | |
1BK6 | X-ray | 2.80 | A/B | 89-510 | [»] | |
1EE4 | X-ray | 2.10 | A/B | 87-509 | [»] | |
1EE5 | X-ray | 2.40 | A | 87-510 | [»] | |
1UN0 | X-ray | 2.60 | A/B | 88-530 | [»] | |
1WA5 | X-ray | 2.00 | B | 1-530 | [»] | |
2C1T | X-ray | 2.60 | A/B | 88-541 | [»] | |
4PVZ | X-ray | 2.50 | A/B | 88-509 | [»] | |
4XZR | X-ray | 2.25 | B | 88-509 | [»] | |
5H2W | X-ray | 2.50 | A/C | 88-510 | [»] | |
5H2X | X-ray | 2.20 | A | 88-510 | [»] | |
5T94 | X-ray | 2.63 | B | 1-542 | [»] | |
SMRi | Q02821 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35644, 422 interactors |
ComplexPortali | CPX-1068, Importin complex, KAP60-KAP95 |
DIPi | DIP-728N |
ELMi | Q02821 |
IntActi | Q02821, 142 interactors |
MINTi | Q02821 |
STRINGi | 4932.YNL189W |
PTM databases
iPTMneti | Q02821 |
Proteomic databases
MaxQBi | Q02821 |
PaxDbi | Q02821 |
PRIDEi | Q02821 |
Genome annotation databases
EnsemblFungii | YNL189W_mRNA; YNL189W; YNL189W |
GeneIDi | 855532 |
KEGGi | sce:YNL189W |
Organism-specific databases
EuPathDBi | FungiDB:YNL189W |
SGDi | S000005133, SRP1 |
Phylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01000000214366 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | Q02821 |
OMAi | GEQEANQ |
Miscellaneous databases
EvolutionaryTracei | Q02821 |
PROi | PR:Q02821 |
RNActi | Q02821, protein |
Family and domain databases
DisProti | DP02227 |
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
IDEALi | IID50012 |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 2 hits PS51214, IBB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IMA1_YEAST | |
Accessioni | Q02821Primary (citable) accession number: Q02821 Secondary accession number(s): D6W0Z8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - UniProtKB entry view manual
User manual for the UniProtKB entry view - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD