UniProtKB - Q02784 (GLRX5_YEAST)
Protein
Monothiol glutaredoxin-5, mitochondrial
Gene
GRX5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed:11950925, PubMed:12730244, PubMed:23615440). Receives 2Fe/2S clusters from scaffold protein ISU1 and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed:27532773, PubMed:23615440).4 Publications
Miscellaneous
Present with 6260 molecules/cell in log phase SD medium.1 Publication
Redox potential
E0 is -175 mV.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 52 | GlutathioneBy similarity | 1 | |
Metal bindingi | 60 | Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 104 | Glutathione; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: SGD
- disulfide oxidoreductase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- protein disulfide oxidoreductase activity Source: InterPro
GO - Biological processi
- cellular response to oxidative stress Source: SGD
- protein maturation by [2Fe-2S] cluster transfer Source: SGD
- protein maturation by [4Fe-4S] cluster transfer Source: SGD
- protein maturation by iron-sulfur cluster transfer Source: SGD
- response to osmotic stress Source: SGD
Keywordsi
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1362409, Mitochondrial iron-sulfur cluster biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Monothiol glutaredoxin-5, mitochondrialCurated |
Gene namesi | Name:GRX5Imported Ordered Locus Names:YPL059WImported ORF Names:LPE13W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005980, GRX5 |
VEuPathDBi | FungiDB:YPL059W |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | Mitochondrion1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000011632 | 30 – 150 | Monothiol glutaredoxin-5, mitochondrialAdd BLAST | 121 |
Proteomic databases
MaxQBi | Q02784 |
PaxDbi | Q02784 |
PRIDEi | Q02784 |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 36121, 42 interactors |
DIPi | DIP-1352N |
IntActi | Q02784, 4 interactors |
MINTi | Q02784 |
STRINGi | 4932.YPL059W |
Miscellaneous databases
RNActi | Q02784, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02784 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02784 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 140 | GlutaredoxinPROSITE-ProRule annotationAdd BLAST | 106 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 92 – 96 | Glutathione bindingBy similarity | 5 | |
Regioni | 117 – 118 | Glutathione bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0911, Eukaryota |
GeneTreei | ENSGT00550000075082 |
HOGENOMi | CLU_026126_2_0_1 |
InParanoidi | Q02784 |
OMAi | KGTKLMP |
Family and domain databases
CDDi | cd03028, GRX_PICOT_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002109, Glutaredoxin IPR033658, GRX_PICOT-like IPR004480, Monothiol_GRX-rel IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR10293, PTHR10293, 1 hit |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00365, TIGR00365, 1 hit |
PROSITEi | View protein in PROSITE PS51354, GLUTAREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q02784-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLPKFNPIR SFSPILRAKT LLRYQNRMYL STEIRKAIED AIESAPVVLF
60 70 80 90 100
MKGTPEFPKC GFSRATIGLL GNQGVDPAKF AAYNVLEDPE LREGIKEFSE
110 120 130 140 150
WPTIPQLYVN KEFIGGCDVI TSMARSGELA DLLEEAQALV PEEEEETKDR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39205 Genomic DNA Translation: AAB68306.1 BK006949 Genomic DNA Translation: DAA11371.1 |
PIRi | S60931 |
RefSeqi | NP_015266.1, NM_001183873.1 |
Genome annotation databases
EnsemblFungii | YPL059W_mRNA; YPL059W; YPL059W |
GeneIDi | 856048 |
KEGGi | sce:YPL059W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39205 Genomic DNA Translation: AAB68306.1 BK006949 Genomic DNA Translation: DAA11371.1 |
PIRi | S60931 |
RefSeqi | NP_015266.1, NM_001183873.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GX8 | X-ray | 1.67 | A | 30-150 | [»] | |
SMRi | Q02784 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36121, 42 interactors |
DIPi | DIP-1352N |
IntActi | Q02784, 4 interactors |
MINTi | Q02784 |
STRINGi | 4932.YPL059W |
Proteomic databases
MaxQBi | Q02784 |
PaxDbi | Q02784 |
PRIDEi | Q02784 |
Genome annotation databases
EnsemblFungii | YPL059W_mRNA; YPL059W; YPL059W |
GeneIDi | 856048 |
KEGGi | sce:YPL059W |
Organism-specific databases
SGDi | S000005980, GRX5 |
VEuPathDBi | FungiDB:YPL059W |
Phylogenomic databases
eggNOGi | KOG0911, Eukaryota |
GeneTreei | ENSGT00550000075082 |
HOGENOMi | CLU_026126_2_0_1 |
InParanoidi | Q02784 |
OMAi | KGTKLMP |
Enzyme and pathway databases
Reactomei | R-SCE-1362409, Mitochondrial iron-sulfur cluster biogenesis |
Miscellaneous databases
EvolutionaryTracei | Q02784 |
PROi | PR:Q02784 |
RNActi | Q02784, protein |
Family and domain databases
CDDi | cd03028, GRX_PICOT_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002109, Glutaredoxin IPR033658, GRX_PICOT-like IPR004480, Monothiol_GRX-rel IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR10293, PTHR10293, 1 hit |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00365, TIGR00365, 1 hit |
PROSITEi | View protein in PROSITE PS51354, GLUTAREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLRX5_YEAST | |
Accessioni | Q02784Primary (citable) accession number: Q02784 Secondary accession number(s): D6W3V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families