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Entry version 164 (13 Feb 2019)
Sequence version 1 (01 Nov 1996)
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Protein

Vacuolar protein sorting-associated protein 28

Gene

VPS28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for normal endocytic and biosynthetic traffic to the yeast vacuole.

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-33974-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28
Alternative name(s):
ESCRT-I complex subunit VPS28
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:VPS28
Synonyms:VPT28
Ordered Locus Names:YPL065W
ORF Names:LPE5W
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Eukaryotic Pathogen Database Resources

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EuPathDBi
FungiDB:YPL065W

Saccharomyces Genome Database

More...
SGDi
S000005986 VPS28

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi40L → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-44. 1 Publication1
Mutagenesisi44Y → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-40. 1 Publication1
Mutagenesisi228 – 231FDLE → ASLA: Abolishes interaction with VPS20. 1 Publication4

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001209581 – 242Vacuolar protein sorting-associated protein 28Add BLAST242

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q02767

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q02767

PRoteomics IDEntifications database

More...
PRIDEi
Q02767

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Self-associates. Interacts with VPS27; the interaction mediates the association with the ESCRT-0 complex. Interacts with VPS20; the interaction mediates the association with the ESCRT-III complex.4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
36114, 109 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-940 ESCRT-I complex

Database of interacting proteins

More...
DIPi
DIP-5828N

Protein interaction database and analysis system

More...
IntActi
Q02767, 6 interactors

Molecular INTeraction database

More...
MINTi
Q02767

STRING: functional protein association networks

More...
STRINGi
4932.YPL065W

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60B/E1-147[»]
2F66X-ray2.80B/E13-125[»]
2F6MX-ray2.10B/D13-118[»]
2G3KX-ray3.05A/B/C/D/E/F/G148-241[»]
2J9UX-ray2.00A/C148-242[»]
2J9VX-ray2.00A148-242[»]
2P22X-ray2.70B1-118[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q02767

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q02767

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q02767

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini11 – 125VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST115
Domaini148 – 242VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni148 – 242Interaction with VSP36 and VPS201 PublicationAdd BLAST95

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000007486

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000203818

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q02767

KEGG Orthology (KO)

More...
KOi
K12184

Identification of Orthologs from Complete Genome Data

More...
OMAi
FKGSKDW

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.120.1130, 1 hit
1.20.1440.200, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR007143 Vps28
IPR017899 VPS28_C
IPR037206 VPS28_C_sf
IPR017898 VPS28_N
IPR038358 VPS28_N_sf

The PANTHER Classification System

More...
PANTHERi
PTHR12937 PTHR12937, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03997 VPS28, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF017535 VPS28, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF140111 SSF140111, 1 hit
SSF140427 SSF140427, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51310 VPS28_C, 1 hit
PS51313 VPS28_N, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q02767-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQKHNIKLNQ NQDISQLFHD EVPLFDNSIT SKDKEVIETL SEIYSIVITL
60 70 80 90 100
DHVEKAYLKD SIDDTQYTNT VDKLLKQFKV YLNSQNKEEI NKHFQSIEAF
110 120 130 140 150
CDTYNITASN AITRLERGIP ITAEHAISTT TSAPSGDNKQ SSSSDKKFNA
160 170 180 190 200
KYVAEATGNF ITVMDALKLN YNAKDQLHPL LAELLISINR VTRDDFENRS
210 220 230 240
KLIDWIVRIN KLSIGDTLTE TQIRELLFDL ELAYKSFYAL LD
Length:242
Mass (Da):27,702
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDE091D3F60C9D132
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti193R → K in AAT92809 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U50630 Genomic DNA Translation: AAB40936.1
U39205 Genomic DNA Translation: AAB68300.1
AY692790 Genomic DNA Translation: AAT92809.1
BK006949 Genomic DNA Translation: DAA11366.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S60925

NCBI Reference Sequences

More...
RefSeqi
NP_015260.1, NM_001183879.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YPL065W_mRNA; YPL065W_mRNA; YPL065W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
856040

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YPL065W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50630 Genomic DNA Translation: AAB40936.1
U39205 Genomic DNA Translation: AAB68300.1
AY692790 Genomic DNA Translation: AAT92809.1
BK006949 Genomic DNA Translation: DAA11366.1
PIRiS60925
RefSeqiNP_015260.1, NM_001183879.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60B/E1-147[»]
2F66X-ray2.80B/E13-125[»]
2F6MX-ray2.10B/D13-118[»]
2G3KX-ray3.05A/B/C/D/E/F/G148-241[»]
2J9UX-ray2.00A/C148-242[»]
2J9VX-ray2.00A148-242[»]
2P22X-ray2.70B1-118[»]
ProteinModelPortaliQ02767
SMRiQ02767
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36114, 109 interactors
ComplexPortaliCPX-940 ESCRT-I complex
DIPiDIP-5828N
IntActiQ02767, 6 interactors
MINTiQ02767
STRINGi4932.YPL065W

Proteomic databases

MaxQBiQ02767
PaxDbiQ02767
PRIDEiQ02767

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL065W_mRNA; YPL065W_mRNA; YPL065W
GeneIDi856040
KEGGisce:YPL065W

Organism-specific databases

EuPathDBiFungiDB:YPL065W
SGDiS000005986 VPS28

Phylogenomic databases

GeneTreeiENSGT00390000007486
HOGENOMiHOG000203818
InParanoidiQ02767
KOiK12184
OMAiFKGSKDW

Enzyme and pathway databases

BioCyciYEAST:G3O-33974-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ02767

Protein Ontology

More...
PROi
PR:Q02767

Family and domain databases

Gene3Di1.20.120.1130, 1 hit
1.20.1440.200, 1 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR007143 Vps28
IPR017899 VPS28_C
IPR037206 VPS28_C_sf
IPR017898 VPS28_N
IPR038358 VPS28_N_sf
PANTHERiPTHR12937 PTHR12937, 1 hit
PfamiView protein in Pfam
PF03997 VPS28, 1 hit
PIRSFiPIRSF017535 VPS28, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF140427 SSF140427, 1 hit
PROSITEiView protein in PROSITE
PS51310 VPS28_C, 1 hit
PS51313 VPS28_N, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiVPS28_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q02767
Secondary accession number(s): D6W3V0, E9P8X2, P87328
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 13, 2019
This is version 164 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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