UniProtKB - Q02742 (GCNT1_HUMAN)
Protein
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Gene
GCNT1
Organism
Homo sapiens (Human)
Status
Functioni
Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine moiety onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. Mucin-type core 2 O-glycans play an important role in leukocyte extravasation as they serve as scaffolds for the display of the selectin ligand sialyl Lewis X by leukocytes.1 Publication
Catalytic activityi
- O3-[β-D-galactosyl-(1→3)-N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-{β-D-galactosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→6)]-N-acetyl-α-D-galactosaminyl}-L-seryl-[protein] + H+ + UDPEC:2.4.1.102
- O3-[β-D-galactosyl-(1→3)-N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-{β-D-galactosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→6)]-N-acetyl-α-D-galactosaminyl}-L-threonyl-[protein] + H+ + UDPEC:2.4.1.102
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 320 | By similarity | 1 |
GO - Molecular functioni
- beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: ProtInc
GO - Biological processi
- cell adhesion molecule production Source: UniProtKB
- glycoprotein biosynthetic process Source: UniProtKB
- kidney morphogenesis Source: Ensembl
- leukocyte tethering or rolling Source: UniProtKB
- O-glycan processing Source: Reactome
- response to insulin Source: Ensembl
- tissue morphogenesis Source: Ensembl
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05939-MONOMER |
BRENDAi | 2.4.1.102, 2681 |
PathwayCommonsi | Q02742 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q02742 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT14, Glycosyltransferase Family 14 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102)Alternative name(s): Core 2-branching enzyme Core2-GlcNAc-transferase Short name: C2GNT Short name: Core 2 GNT |
Gene namesi | Name:GCNT1 Synonyms:NACGT2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4203, GCNT1 |
MIMi | 600391, gene |
neXtProti | NX_Q02742 |
VEuPathDBi | HostDB:ENSG00000187210.12 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: Also detected in the trans-Golgi network.
Extracellular region or secreted
- extracellular space Source: Ensembl
Golgi apparatus
- Golgi cisterna Source: UniProtKB
- Golgi membrane Source: Reactome
- trans-Golgi network Source: UniProtKB
Other locations
- integral component of membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 32 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 33 – 428 | LumenalSequence analysisAdd BLAST | 396 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 – 6 | LL → AA: Loss of interaction with GOLPH3 and loss of localization to the Golgi. 1 Publication | 2 | |
Mutagenesisi | 7 – 9 | RRR → AAA: Loss of interaction with GOLPH3 and loss of localization to the Golgi. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 2650 |
OpenTargetsi | ENSG00000187210 |
PharmGKBi | PA168 |
Miscellaneous databases
Pharosi | Q02742, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2321632 |
Genetic variation databases
BioMutai | GCNT1 |
DMDMi | 218512053 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191395 | 1 – 428 | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferaseAdd BLAST | 428 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 58 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 59 ↔ 413 | By similarity | ||
Glycosylationi | 95 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 100 ↔ 172 | By similarity | ||
Disulfide bondi | 151 ↔ 199 | By similarity | ||
Disulfide bondi | 372 ↔ 381 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q02742 |
jPOSTi | Q02742 |
MassIVEi | Q02742 |
MaxQBi | Q02742 |
PaxDbi | Q02742 |
PeptideAtlasi | Q02742 |
PRIDEi | Q02742 |
ProteomicsDBi | 58117 |
PTM databases
GlyGeni | Q02742, 2 sites |
iPTMneti | Q02742 |
PhosphoSitePlusi | Q02742 |
Expressioni
Tissue specificityi
Highly expressed in activated T-lymphocytes and myeloid cells.
Gene expression databases
Bgeei | ENSG00000187210, Expressed in duodenum and 173 other tissues |
Genevisiblei | Q02742, HS |
Organism-specific databases
HPAi | ENSG00000187210, Tissue enhanced (intestine, parathyroid gland) |
Interactioni
Subunit structurei
Interacts with GOLPH3; may control GCNT1 retention in the Golgi.
1 PublicationBinary interactionsi
Q02742
With | #Exp. | IntAct |
---|---|---|
GOLPH3 [Q9H4A6] | 6 | EBI-8766035,EBI-2465479 |
Protein-protein interaction databases
BioGRIDi | 108920, 12 interactors |
IntActi | Q02742, 19 interactors |
STRINGi | 9606.ENSP00000415454 |
Miscellaneous databases
RNActi | Q02742, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 5 – 9 | Mediates interaction with GOLPH3 and is necessary and sufficient for localization to the Golgi1 Publication | 5 | |
Regioni | 33 – 121 | Stem regionBy similarityAdd BLAST | 89 | |
Regioni | 122 – 428 | CatalyticBy similarityAdd BLAST | 307 |
Sequence similaritiesi
Belongs to the glycosyltransferase 14 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0799, Eukaryota |
GeneTreei | ENSGT00940000161348 |
HOGENOMi | CLU_032341_1_2_1 |
InParanoidi | Q02742 |
OMAi | PSSNINC |
OrthoDBi | 868849at2759 |
PhylomeDBi | Q02742 |
TreeFami | TF315534 |
Family and domain databases
InterProi | View protein in InterPro IPR003406, Glyco_trans_14 |
Pfami | View protein in Pfam PF02485, Branch, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02742-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRTLLRRRL FSYPTKYYFM VLVLSLITFS VLRIHQKPEF VSVRHLELAG
60 70 80 90 100
ENPSSDINCT KVLQGDVNEI QKVKLEILTV KFKKRPRWTP DDYINMTSDC
110 120 130 140 150
SSFIKRRKYI VEPLSKEEAE FPIAYSIVVH HKIEMLDRLL RAIYMPQNFY
160 170 180 190 200
CIHVDTKSED SYLAAVMGIA SCFSNVFVAS RLESVVYASW SRVQADLNCM
210 220 230 240 250
KDLYAMSANW KYLINLCGMD FPIKTNLEIV RKLKLLMGEN NLETERMPSH
260 270 280 290 300
KEERWKKRYE VVNGKLTNTG TVKMLPPLET PLFSGSAYFV VSREYVGYVL
310 320 330 340 350
QNEKIQKLME WAQDTYSPDE YLWATIQRIP EVPGSLPASH KYDLSDMQAV
360 370 380 390 400
ARFVKWQYFE GDVSKGAPYP PCDGVHVRSV CIFGAGDLNW MLRKHHLFAN
410 420
KFDVDVDLFA IQCLDEHLRH KALETLKH
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048000 | 152 | I → V2 PublicationsCorresponds to variant dbSNP:rs2282683Ensembl. | 1 | |
Natural variantiVAR_048001 | 158 | S → C. Corresponds to variant dbSNP:rs11546569Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97347 mRNA Translation: AAA35919.1 L41415 Genomic DNA Translation: AAA96661.1 AL161626 Genomic DNA No translation available. CH471089 Genomic DNA Translation: EAW62580.1 BC074885 mRNA Translation: AAH74885.1 BC074886 mRNA Translation: AAH74886.1 BC109101 mRNA Translation: AAI09102.1 BC109102 mRNA Translation: AAI09103.1 |
CCDSi | CCDS6653.1 |
PIRi | A46293 |
RefSeqi | NP_001091102.1, NM_001097633.1 NP_001091103.1, NM_001097634.1 NP_001091104.1, NM_001097635.1 NP_001091105.1, NM_001097636.1 NP_001481.2, NM_001490.4 XP_016870109.1, XM_017014620.1 XP_016870110.1, XM_017014621.1 |
Genome annotation databases
Ensembli | ENST00000376730; ENSP00000365920; ENSG00000187210 ENST00000442371; ENSP00000415454; ENSG00000187210 ENST00000444201; ENSP00000390703; ENSG00000187210 |
GeneIDi | 2650 |
KEGGi | hsa:2650 |
UCSCi | uc004akf.5, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97347 mRNA Translation: AAA35919.1 L41415 Genomic DNA Translation: AAA96661.1 AL161626 Genomic DNA No translation available. CH471089 Genomic DNA Translation: EAW62580.1 BC074885 mRNA Translation: AAH74885.1 BC074886 mRNA Translation: AAH74886.1 BC109101 mRNA Translation: AAI09102.1 BC109102 mRNA Translation: AAI09103.1 |
CCDSi | CCDS6653.1 |
PIRi | A46293 |
RefSeqi | NP_001091102.1, NM_001097633.1 NP_001091103.1, NM_001097634.1 NP_001091104.1, NM_001097635.1 NP_001091105.1, NM_001097636.1 NP_001481.2, NM_001490.4 XP_016870109.1, XM_017014620.1 XP_016870110.1, XM_017014621.1 |
3D structure databases
SMRi | Q02742 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 108920, 12 interactors |
IntActi | Q02742, 19 interactors |
STRINGi | 9606.ENSP00000415454 |
Chemistry databases
ChEMBLi | CHEMBL2321632 |
Protein family/group databases
CAZyi | GT14, Glycosyltransferase Family 14 |
PTM databases
GlyGeni | Q02742, 2 sites |
iPTMneti | Q02742 |
PhosphoSitePlusi | Q02742 |
Genetic variation databases
BioMutai | GCNT1 |
DMDMi | 218512053 |
Proteomic databases
EPDi | Q02742 |
jPOSTi | Q02742 |
MassIVEi | Q02742 |
MaxQBi | Q02742 |
PaxDbi | Q02742 |
PeptideAtlasi | Q02742 |
PRIDEi | Q02742 |
ProteomicsDBi | 58117 |
Protocols and materials databases
Antibodypediai | 27275, 219 antibodies |
DNASUi | 2650 |
Genome annotation databases
Ensembli | ENST00000376730; ENSP00000365920; ENSG00000187210 ENST00000442371; ENSP00000415454; ENSG00000187210 ENST00000444201; ENSP00000390703; ENSG00000187210 |
GeneIDi | 2650 |
KEGGi | hsa:2650 |
UCSCi | uc004akf.5, human |
Organism-specific databases
CTDi | 2650 |
DisGeNETi | 2650 |
GeneCardsi | GCNT1 |
HGNCi | HGNC:4203, GCNT1 |
HPAi | ENSG00000187210, Tissue enhanced (intestine, parathyroid gland) |
MIMi | 600391, gene |
neXtProti | NX_Q02742 |
OpenTargetsi | ENSG00000187210 |
PharmGKBi | PA168 |
VEuPathDBi | HostDB:ENSG00000187210.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0799, Eukaryota |
GeneTreei | ENSGT00940000161348 |
HOGENOMi | CLU_032341_1_2_1 |
InParanoidi | Q02742 |
OMAi | PSSNINC |
OrthoDBi | 868849at2759 |
PhylomeDBi | Q02742 |
TreeFami | TF315534 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | MetaCyc:HS05939-MONOMER |
BRENDAi | 2.4.1.102, 2681 |
PathwayCommonsi | Q02742 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q02742 |
Miscellaneous databases
BioGRID-ORCSi | 2650, 5 hits in 988 CRISPR screens |
ChiTaRSi | GCNT1, human |
GeneWikii | GCNT1 |
GenomeRNAii | 2650 |
Pharosi | Q02742, Tbio |
PROi | PR:Q02742 |
RNActi | Q02742, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000187210, Expressed in duodenum and 173 other tissues |
Genevisiblei | Q02742, HS |
Family and domain databases
InterProi | View protein in InterPro IPR003406, Glyco_trans_14 |
Pfami | View protein in Pfam PF02485, Branch, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCNT1_HUMAN | |
Accessioni | Q02742Primary (citable) accession number: Q02742 Secondary accession number(s): Q6DJZ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | December 16, 2008 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families