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Protein

Heavy metal tolerance protein

Gene

hmt1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in metal tolerance. Probably involved in the transport of metal-bound phytochelatins. Compartmentalizes cadmium within vacuoles, thereby protecting cells from cadmium toxicity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei542Glutathione; via amide nitrogenBy similarity1
Binding sitei593ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi617 – 628ATPPROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: PomBase
  • ATP binding Source: PomBase
  • phytochelatin transmembrane transporter ATPase activity Source: PomBase

GO - Biological processi

  • cadmium ion import into vacuole Source: PomBase
  • cellular detoxification of cadmium ion Source: PomBase
  • glutathione transmembrane import into vacuole Source: PomBase
  • phytochelatin 2 import into vacuole Source: PomBase
  • phytochelatin import into vacuole Source: PomBase

Keywordsi

Biological processCadmium resistance, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-1369007 Mitochondrial ABC transporters

Protein family/group databases

TCDBi3.A.1.210.2 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Heavy metal tolerance protein
Gene namesi
Name:hmt1
ORF Names:SPCC737.09c, SPCC74.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC737.09c
PomBaseiSPCC737.09c hmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei51 – 71HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei88 – 108HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei126 – 146HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei156 – 176HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei263 – 283HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei304 – 324HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei403 – 423HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei490 – 511HelicalPROSITE-ProRule annotationAdd BLAST22

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000000025928 – 830Heavy metal tolerance proteinAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi150N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi350N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ02592
PaxDbiQ02592
PRIDEiQ02592

Interactioni

Protein-protein interaction databases

BioGridi280210, 19 interactors
STRINGi4896.SPCC737.09c.1

Structurei

3D structure databases

ProteinModelPortaliQ02592
SMRiQ02592
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 550ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST286
Domaini584 – 818ABC transporterPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni429 – 433Glutathione bindingBy similarity5
Regioni492 – 495Glutathione bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ02592
OMAiSNLWISV
OrthoDBiEOG092C3H79
PhylomeDBiQ02592

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRYNSPRL NILELVLLYV GFFSIGSLNL LQKRKATSDP YRRKNRFGKE
60 70 80 90 100
PIGIISWWIL GIALTYVVDI SNLVIYALRV PNWWPCKTTV VCLILFLLFW
110 120 130 140 150
IIVLISCADS KALPKNADSI LKAYRLSVLY VWAIDIVFET IFIVYSPHPN
160 170 180 190 200
ETFQGIVLAD HVARLVLCVF ATAIYLTYRR KRHTHDPLDF EERQLTEESN
210 220 230 240 250
VNENAISQNP STVQLGVSAS TSNFGTLKST SKKPSDKSWA EYFRSFSTLL
260 270 280 290 300
PYLWPTKDYR LQFQIFICIV LLFLGRAVNI LAPRQLGVLT EKLTKHSEKI
310 320 330 340 350
PWSDVILFVI YRFLQGNMGV IGSLRSFLWV PVSQYAYRAI STKALRHVLN
360 370 380 390 400
LSYDFHLNKR AGEVLTALTK GSSLNTFAEQ VVFQIGPVLL DLGVAMVYFF
410 420 430 440 450
IKFDIYFTLI VLIMTLCYCY VTVKITSWRT EARRKMVNSW RESYAVQNDA
460 470 480 490 500
IMNFETVKNF DADDFENERY GHAVDIYLKQ ERKVLFSLNF LNIVQGGIFT
510 520 530 540 550
FSLAIACLLS AYRVTFGFNT VGDFVILLTY MIQLQQPLNF FGTLYRSLQN
560 570 580 590 600
SIIDTERLLE IFEEKPTVVE KPNAPDLKVT QGKVIFSHVS FAYDPRKPVL
610 620 630 640 650
SDINFVAQPG KVIALVGESG GGKSTIMRIL LRFFDVNSGS ITIDDQDIRN
660 670 680 690 700
VTLSSLRSSI GVVPQDSTLF NDTILYNIKY AKPSATNEEI YAAAKAAQIH
710 720 730 740 750
DRILQFPDGY NSRVGERGLK LSGGEKQRVA VARAILKDPS IILLDEATSA
760 770 780 790 800
LDTNTERQIQ AALNRLASGR TAIVIAHRLS TITNADLILC ISNGRIVETG
810 820 830
THEELIKRDG GAYKKMWFQQ AMGKTSAETH
Length:830
Mass (Da):93,994
Last modified:December 15, 1998 - v3
Checksum:i909FBD10D51F50A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79R → A in CAA78419 (PubMed:1396551).Curated1
Sequence conflicti439S → T in CAA78419 (PubMed:1396551).Curated1
Sequence conflicti812A → R in CAA78419 (PubMed:1396551).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14055 mRNA Translation: CAA78419.1
CU329672 Genomic DNA Translation: CAA20865.1
PIRiS25198
RefSeqiNP_588371.3, NM_001023362.3

Genome annotation databases

EnsemblFungiiSPCC737.09c.1; SPCC737.09c.1:pep; SPCC737.09c
GeneIDi3361134
KEGGispo:SPCC737.09c

Similar proteinsi

Entry informationi

Entry nameiHMT1_SCHPO
AccessioniPrimary (citable) accession number: Q02592
Secondary accession number(s): O13675, Q9UQW7, Q9USI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 15, 1998
Last modified: June 20, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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