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UniProtKB - Q02589 (ADPRH_RAT)
Protein
ADP-ribosylhydrolase ARH1
Gene
Adprh
Organism
Rattus norvegicus (Rat)
Status
Functioni
Specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.
By similarityCatalytic activityi
- EC:3.2.2.19By similarity
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
Activity regulationi
Its activity is synergistically stimulated by magnesium and dithiothreitol (DTT) in vitro.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 59 | Magnesium 1By similarity | 1 | |
Metal bindingi | 60 | Magnesium 1By similarity | 1 | |
Metal bindingi | 61 | Magnesium 1By similarity | 1 | |
Binding sitei | 90 | SubstrateBy similarity | 1 | |
Binding sitei | 129 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 135 | Substrate; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 307 | Magnesium 2By similarity | 1 | |
Metal bindingi | 309 | Magnesium 1By similarity | 1 | |
Metal bindingi | 309 | Magnesium 2By similarity | 1 | |
Metal bindingi | 310 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- ADP-ribosylarginine hydrolase activity Source: RGD
- magnesium ion binding Source: UniProtKB
- potassium ion binding Source: UniProtKB
GO - Biological processi
- cellular protein modification process Source: UniProtKB
- protein de-ADP-ribosylation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylhydrolase ARH1Curated (EC:3.2.2.19By similarity)Alternative name(s): ADP-ribose-L-arginine cleaving enzyme [Protein ADP-ribosylarginine] hydrolase Short name: ADP-ribosylarginine hydrolase |
Gene namesi | Name:Adprh Synonyms:Arh1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2052, Adprh |
Subcellular locationi
Cytosol
- cytosol Source: RGD
Extracellular region or secreted
- extracellular space Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157285 | 1 – 362 | ADP-ribosylhydrolase ARH1Add BLAST | 362 |
Proteomic databases
jPOSTi | Q02589 |
PaxDbi | Q02589 |
PTM databases
iPTMneti | Q02589 |
PhosphoSitePlusi | Q02589 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000027260, Expressed in brain and 22 other tissues |
Genevisiblei | Q02589, RN |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000034815 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 108 | SubstrateBy similarity | 3 | |
Regioni | 168 – 170 | SubstrateBy similarity | 3 | |
Regioni | 268 – 270 | SubstrateBy similarity | 3 | |
Regioni | 274 – 275 | SubstrateBy similarity | 2 |
Sequence similaritiesi
Belongs to the ADP-ribosylglycohydrolase family.Curated
Phylogenomic databases
eggNOGi | ENOG502QPMI, Eukaryota |
GeneTreei | ENSGT00530000063627 |
HOGENOMi | CLU_047061_0_0_1 |
InParanoidi | Q02589 |
OMAi | ACWWGAM |
OrthoDBi | 1112828at2759 |
PhylomeDBi | Q02589 |
TreeFami | TF329417 |
Family and domain databases
Gene3Di | 1.10.4080.10, 1 hit |
InterProi | View protein in InterPro IPR012108, ADP-ribosylarg_hydro IPR005502, Ribosyl_crysJ1 IPR036705, Ribosyl_crysJ1_sf |
Pfami | View protein in Pfam PF03747, ADP_ribosyl_GH, 1 hit |
PIRSFi | PIRSF016939, ADP_ribslarg_hdr, 1 hit |
SUPFAMi | SSF101478, SSF101478, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02589-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGGLIERYV AAMVLSAAGD TLGYFNGKWE FLRDGEKIHR QLAQMGDLEA
60 70 80 90 100
IDVAQWRVSD DTIMHLATAE ALMEAGSSPD LPQLYSLLAK HYRDCMGDMD
110 120 130 140 150
GRAPGGACMQ NAMQLDPDRA DGWRIPFNSH EGGCGAAMRA MCIGLRFPHP
160 170 180 190 200
SQLDTLIQVS IESGRMTHHH PTGYLGSLAS ALFTAYAVNG KSPRQWGKGL
210 220 230 240 250
MEVLPEAKAY VTQSGYFVKE NLQHWSYFEK EWEKYLELRG ILDGKSAPVF
260 270 280 290 300
PKPFGVKERD QFYIEVSYSG WGGSSGHDAP MIAYDALLAA GDSWKELAHR
310 320 330 340 350
AFFHGGDSDS TATIAGCWWG VMHGFKGVNP SNYEKLEYRQ RLEEAGRALY
360
SLGSKEDTIL GP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 252 | K → Q in AAA40691 (PubMed:1375222).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86341 mRNA Translation: AAA40691.1 BC082065 mRNA Translation: AAH82065.1 |
PIRi | A38135 |
RefSeqi | NP_899154.2, NM_183325.2 XP_006248422.1, XM_006248360.3 |
Genome annotation databases
Ensembli | ENSRNOT00000038439; ENSRNOP00000034815; ENSRNOG00000027260 |
GeneIDi | 25371 |
KEGGi | rno:25371 |
UCSCi | RGD:2052, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86341 mRNA Translation: AAA40691.1 BC082065 mRNA Translation: AAH82065.1 |
PIRi | A38135 |
RefSeqi | NP_899154.2, NM_183325.2 XP_006248422.1, XM_006248360.3 |
3D structure databases
AlphaFoldDBi | Q02589 |
SMRi | Q02589 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000034815 |
PTM databases
iPTMneti | Q02589 |
PhosphoSitePlusi | Q02589 |
Proteomic databases
jPOSTi | Q02589 |
PaxDbi | Q02589 |
Protocols and materials databases
DNASUi | 25371 |
Genome annotation databases
Ensembli | ENSRNOT00000038439; ENSRNOP00000034815; ENSRNOG00000027260 |
GeneIDi | 25371 |
KEGGi | rno:25371 |
UCSCi | RGD:2052, rat |
Organism-specific databases
CTDi | 141 |
RGDi | 2052, Adprh |
Phylogenomic databases
eggNOGi | ENOG502QPMI, Eukaryota |
GeneTreei | ENSGT00530000063627 |
HOGENOMi | CLU_047061_0_0_1 |
InParanoidi | Q02589 |
OMAi | ACWWGAM |
OrthoDBi | 1112828at2759 |
PhylomeDBi | Q02589 |
TreeFami | TF329417 |
Miscellaneous databases
PROi | PR:Q02589 |
Gene expression databases
Bgeei | ENSRNOG00000027260, Expressed in brain and 22 other tissues |
Genevisiblei | Q02589, RN |
Family and domain databases
Gene3Di | 1.10.4080.10, 1 hit |
InterProi | View protein in InterPro IPR012108, ADP-ribosylarg_hydro IPR005502, Ribosyl_crysJ1 IPR036705, Ribosyl_crysJ1_sf |
Pfami | View protein in Pfam PF03747, ADP_ribosyl_GH, 1 hit |
PIRSFi | PIRSF016939, ADP_ribslarg_hdr, 1 hit |
SUPFAMi | SSF101478, SSF101478, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADPRH_RAT | |
Accessioni | Q02589Primary (citable) accession number: Q02589 Secondary accession number(s): Q66H27 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | May 29, 2007 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families