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Protein

Manganese peroxidase 1

Gene

MNP1

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of Mn2+ to Mn3+. The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.

Catalytic activityi

2 Mn2+ + 2 H+ + H2O2 = 2 Mn3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 2 calcium ions per subunit.
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Manganese1
Metal bindingi60Manganese1
Sitei63Transition state stabilizer1
Active sitei67Proton acceptor1
Metal bindingi68Calcium 11
Metal bindingi83Calcium 1; via carbonyl oxygen1
Metal bindingi85Calcium 11
Metal bindingi87Calcium 11
Metal bindingi194Iron (heme axial ligand)1
Metal bindingi195Calcium 21
Metal bindingi200Manganese1
Metal bindingi212Calcium 21
Metal bindingi214Calcium 21
Metal bindingi217Calcium 2; via carbonyl oxygen1
Metal bindingi219Calcium 21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide, Lignin degradation
LigandCalcium, Heme, Iron, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14335
BRENDAi1.11.1.13 1380
SABIO-RKiQ02567

Protein family/group databases

CAZyiAA2 Auxiliary Activities 2
mycoCLAPiMPO2A_PHACH
PeroxiBasei2379 PcMnP01-1A_RP78
3866 PcMnP01_OGC101

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese peroxidase 1 (EC:1.11.1.13)
Short name:
MnP-1
Short name:
MnP1
Alternative name(s):
Manganese peroxidase isozyme 1
Peroxidase manganese-dependent 1
Peroxidase manganese-dependent I
Gene namesi
Name:MNP1
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000002377822 – 378Manganese peroxidase 1Add BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 36
Disulfide bondi35 ↔ 310
Disulfide bondi54 ↔ 138
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi152N-linked (GlcNAc...) asparagine1
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi274 ↔ 340
Disulfide bondi362 ↔ 369

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ02567

Expressioni

Inductioni

During wound-healing and by factors which induce suberization.

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ02567
SMRiQ02567
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02567

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZ9A Eukaryota
ENOG410YEPY LUCA
OMAiFRTACEW

Family and domain databases

CDDicd00692 ligninase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR001621 Ligninase
IPR024589 Ligninase_C
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PF11895 Peroxidase_ext, 1 hit
PRINTSiPR00462 LIGNINASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02567-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFKSLIAFV ALAAAVRAAP TAVCPDGTRV SHAACCAFIP LAQDLQETIF
60 70 80 90 100
QNECGEDAHE VIRLTFHDAI AISRSQGPKA GGGADGSMLL FPTVEPNFSA
110 120 130 140 150
NNGIDDSVNN LIPFMQKHNT ISAADLVQFA GAVALSNCPG APRLEFLAGR
160 170 180 190 200
PNKTIAAVDG LIPEPQDSVT KILQRFEDAG GFTPFEVVSL LASHSVARAD
210 220 230 240 250
KVDQTIDAAP FDSTPFTFDT QVFLEVLLKG VGFPGSANNT GEVASPLPLG
260 270 280 290 300
SGSDTGEMRL QSDFALAHDP RTACIWQGFV NEQAFMAASF RAAMSKLAVL
310 320 330 340 350
GHNRNSLIDC SDVVPVPKPA TGQPAMFPAS TGPQDLELSC PSERFPTLTT
360 370
QPGASQSLIA HCPDGSMSCP GVQFNGPA
Length:378
Mass (Da):39,557
Last modified:November 1, 1996 - v1
Checksum:i17A9A8F642441F27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75S → L in AAA33742 (PubMed:2925681).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60672 Genomic DNA Translation: AAA33744.1
M77513 Genomic DNA Translation: AAA33743.1
J04624 mRNA Translation: AAA33742.1
PIRiJN0092 A33271

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60672 Genomic DNA Translation: AAA33744.1
M77513 Genomic DNA Translation: AAA33743.1
J04624 mRNA Translation: AAA33742.1
PIRiJN0092 A33271

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MN1X-ray2.00A22-378[»]
1MN2X-ray2.00A22-378[»]
1MNPX-ray2.00A22-378[»]
1YYDX-ray1.45A22-378[»]
1YYGX-ray1.60A22-378[»]
1YZPX-ray1.60A22-378[»]
1YZRX-ray1.60A22-378[»]
3M5QX-ray0.93A22-378[»]
3M8MX-ray1.05A22-378[»]
ProteinModelPortaliQ02567
SMRiQ02567
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA2 Auxiliary Activities 2
mycoCLAPiMPO2A_PHACH
PeroxiBasei2379 PcMnP01-1A_RP78
3866 PcMnP01_OGC101

Proteomic databases

PRIDEiQ02567

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IZ9A Eukaryota
ENOG410YEPY LUCA
OMAiFRTACEW

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14335
BRENDAi1.11.1.13 1380
SABIO-RKiQ02567

Miscellaneous databases

EvolutionaryTraceiQ02567

Family and domain databases

CDDicd00692 ligninase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR001621 Ligninase
IPR024589 Ligninase_C
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PF11895 Peroxidase_ext, 1 hit
PRINTSiPR00462 LIGNINASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEM1_PHACH
AccessioniPrimary (citable) accession number: Q02567
Secondary accession number(s): Q01788
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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