UniProtKB - Q02555 (RNT1_YEAST)
Protein
Ribonuclease 3
Gene
RNT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
DsRNA-specific nuclease that cleaves eukaryotic pre-ribosomal RNA at the U3 snoRNP-dependent A0 site in the 5'-external transcribed spacer (ETS) and in the 3'-ETS. In vitro, cleaves synthetic 5'-ETS RNA A0 site in the absence of snoRNA or other factors. Has an essential growth function in addition to pre-rRNA processing.
Miscellaneous
Present with 4970 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.3
GO - Molecular functioni
- double-stranded RNA binding Source: GO_Central
- ribonuclease III activity Source: SGD
GO - Biological processi
- box C/D snoRNA processing Source: SGD
- box H/ACA snoRNA processing Source: SGD
- chromatin organization Source: SGD
- nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay Source: SGD
- pre-miRNA processing Source: GO_Central
- primary miRNA processing Source: GO_Central
- production of siRNA involved in RNA interference Source: GO_Central
- regulation of fungal-type cell wall organization Source: SGD
- RNA processing Source: GO_Central
- rRNA processing Source: SGD
- rRNA transcription Source: SGD
- termination of RNA polymerase II transcription, exosome-dependent Source: SGD
- U1 snRNA 3'-end processing Source: SGD
- U4 snRNA 3'-end processing Source: SGD
- U5 snRNA 3'-end processing Source: SGD
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease, RNA-binding |
Enzyme and pathway databases
BRENDAi | 3.1.26.3, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease 3 (EC:3.1.26.3)Alternative name(s): Ribonuclease III Short name: RNase III |
Gene namesi | Name:RNT1 Ordered Locus Names:YMR239C ORF Names:YM9408.01C, YM9959.21 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YMR239C |
SGDi | S000004852, RNT1 |
Subcellular locationi
Nucleus
- nucleolus Source: SGD
- nucleoplasm Source: SGD
- nucleus Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180466 | 1 – 471 | Ribonuclease 3Add BLAST | 471 |
Proteomic databases
MaxQBi | Q02555 |
PaxDbi | Q02555 |
PRIDEi | Q02555 |
PTM databases
iPTMneti | Q02555 |
Interactioni
Binary interactionsi
Q02555
With | #Exp. | IntAct |
---|---|---|
SEN1 [Q00416] | 2 | EBI-15673,EBI-16945 |
Protein-protein interaction databases
BioGRIDi | 35418, 339 interactors |
DIPi | DIP-4298N |
IntActi | Q02555, 8 interactors |
MINTi | Q02555 |
STRINGi | 4932.YMR239C |
Miscellaneous databases
RNActi | Q02555, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q02555 |
SMRi | Q02555 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02555 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 227 – 331 | RNase IIIPROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 369 – 437 | DRBMPROSITE-ProRule annotationAdd BLAST | 69 |
Phylogenomic databases
eggNOGi | KOG1817, Eukaryota |
HOGENOMi | CLU_026251_0_0_1 |
InParanoidi | Q02555 |
OMAi | SKKKAMA |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd00593, RIBOc, 1 hit |
Gene3Di | 1.10.1520.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR040540, RNase_3_N IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF00636, Ribonuclease_3, 1 hit PF18497, RNase_3_N, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit SM00535, RIBOc, 1 hit |
SUPFAMi | SSF69065, SSF69065, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02555-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSKVAGKKK TQNDNKLDNE NGSQQRENIN TKTLLKGNLK ISNYKYLEVI
60 70 80 90 100
QLEHAVTKLV ESYNKIIELS PNLVAYNEAV NNQDRVPVQI LPSLSRYQLK
110 120 130 140 150
LAAELKTLHD LKKDAILTEI TDYENEFDTE QKQPILQEIS KADMEKLEKL
160 170 180 190 200
EQVKREKREK IDVNVYENLN EKEDEEEDEG EDSYDPTKAG DIVKATKWPP
210 220 230 240 250
KLPEIQDLAI RARVFIHKST IKDKVYLSGS EMINAHNERL EFLGDSILNS
260 270 280 290 300
VMTLIIYNKF PDYSEGQLST LRMNLVSNEQ IKQWSIMYNF HEKLKTNFDL
310 320 330 340 350
KDENSNFQNG KLKLYADVFE AYIGGLMEDD PRNNLPKIRK WLRKLAKPVI
360 370 380 390 400
EEATRNQVAL EKTDKLDMNA KRQLYSLIGY ASLRLHYVTV KKPTAVDPNS
410 420 430 440 450
IVECRVGDGT VLGTGVGRNI KIAGIRAAEN ALRDKKMLDF YAKQRAAIPR
460 470
SESVLKDPSQ KNKKRKFSDT S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27016 Genomic DNA Translation: AAB04172.1 Z48756 Genomic DNA Translation: CAA88649.1 Z49939 Genomic DNA Translation: CAA90210.1 BK006946 Genomic DNA Translation: DAA10139.1 |
PIRi | S56053 |
RefSeqi | NP_013966.1, NM_001182746.1 |
Genome annotation databases
EnsemblFungii | YMR239C_mRNA; YMR239C; YMR239C |
GeneIDi | 855280 |
KEGGi | sce:YMR239C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27016 Genomic DNA Translation: AAB04172.1 Z48756 Genomic DNA Translation: CAA88649.1 Z49939 Genomic DNA Translation: CAA90210.1 BK006946 Genomic DNA Translation: DAA10139.1 |
PIRi | S56053 |
RefSeqi | NP_013966.1, NM_001182746.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T4L | NMR | - | B | 366-453 | [»] | |
1T4N | NMR | - | A | 364-447 | [»] | |
1T4O | X-ray | 2.50 | A/B | 362-471 | [»] | |
2LBS | NMR | - | B | 366-453 | [»] | |
2LUP | NMR | - | B | 366-453 | [»] | |
2LUQ | NMR | - | A | 366-453 | [»] | |
4OOG | X-ray | 2.50 | A/B | 42-151 | [»] | |
C | 197-457 | [»] | ||||
5T16 | X-ray | 2.78 | A/B/I/J | 184-459 | [»] | |
C/D/E/F/K/L/M/N | 41-159 | [»] | ||||
BMRBi | Q02555 | |||||
SMRi | Q02555 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35418, 339 interactors |
DIPi | DIP-4298N |
IntActi | Q02555, 8 interactors |
MINTi | Q02555 |
STRINGi | 4932.YMR239C |
PTM databases
iPTMneti | Q02555 |
Proteomic databases
MaxQBi | Q02555 |
PaxDbi | Q02555 |
PRIDEi | Q02555 |
Genome annotation databases
EnsemblFungii | YMR239C_mRNA; YMR239C; YMR239C |
GeneIDi | 855280 |
KEGGi | sce:YMR239C |
Organism-specific databases
EuPathDBi | FungiDB:YMR239C |
SGDi | S000004852, RNT1 |
Phylogenomic databases
eggNOGi | KOG1817, Eukaryota |
HOGENOMi | CLU_026251_0_0_1 |
InParanoidi | Q02555 |
OMAi | SKKKAMA |
Enzyme and pathway databases
BRENDAi | 3.1.26.3, 984 |
Miscellaneous databases
EvolutionaryTracei | Q02555 |
PROi | PR:Q02555 |
RNActi | Q02555, protein |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd00593, RIBOc, 1 hit |
Gene3Di | 1.10.1520.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR040540, RNase_3_N IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF00636, Ribonuclease_3, 1 hit PF18497, RNase_3_N, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit SM00535, RIBOc, 1 hit |
SUPFAMi | SSF69065, SSF69065, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNT1_YEAST | |
Accessioni | Q02555Primary (citable) accession number: Q02555 Secondary accession number(s): D6W065, Q04008, Q05038 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD