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Protein

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Gene

Mgat3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-(beta-D-GlcNAc-(1->4))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.144 5301
ReactomeiR-RNO-975574 Reactions specific to the hybrid N-glycan synthesis pathway
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT17 Glycosyltransferase Family 17

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (EC:2.4.1.144)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
Short name:
GNT-III
Short name:
GlcNAc-T III
Short name:
N-acetylglucosaminyltransferase III
Gene namesi
Name:Mgat3
Synonyms:Gnt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3084 Mgat3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini24 – 538LumenalSequence analysisAdd BLAST515

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888441 – 538Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferaseAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi245N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi263N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ02527

Expressioni

Gene expression databases

BgeeiENSRNOG00000017434
GenevisibleiQ02527 RN

Interactioni

Subunit structurei

Interacts with MGAT4D.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023434

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi35 – 86Pro-richAdd BLAST52

Sequence similaritiesi

Belongs to the glycosyltransferase 17 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEQJ Eukaryota
ENOG410YQKP LUCA
GeneTreeiENSGT00390000008221
HOGENOMiHOG000113579
HOVERGENiHBG052468
InParanoidiQ02527
KOiK00737
OMAiMKMRRHK
OrthoDBiEOG091G0CDF
PhylomeDBiQ02527
TreeFamiTF323781

Family and domain databases

InterProiView protein in InterPro
IPR006813 Glyco_trans_17
PANTHERiPTHR12224 PTHR12224, 1 hit
PfamiView protein in Pfam
PF04724 Glyco_transf_17, 1 hit

Sequencei

Sequence statusi: Complete.

Q02527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLISSFF
60 70 80 90 100
WNNAPVTPQA SPEPGDPDLL RTPLYSHSPL LQPLSPSKAT EELHRVDFVL
110 120 130 140 150
PEDTTEYFVR TKAGGVCFKP GTRMLEKPSP GRTEEKTKVA EGSSVRGPAR
160 170 180 190 200
RPMRHVLSAR ERLGGRGTRR KWVECVCLPG WHGPSCGVPT VVQYSNLPTK
210 220 230 240 250
ERLVPREVPR RVINAININH EFDLLDVRFH ELGDVVDAFV VCESNFTAYG
260 270 280 290 300
EPRPLKFREM LTNGTFEYIR HKVLYVFLDH FPPGGRQDGW IADDYLRTFL
310 320 330 340 350
TQDGVSRLRN LRPDDVFIID DADEIPARDG VLFLKLYDGW TEPFAFHMRK
360 370 380 390 400
SLYGFFWKQP GTLEVVSGCT IDMLQAVYGL DGIRLRRRQY YTMPNFRQYE
410 420 430 440 450
NRTGHILVQW SLGSPLHFAG WHCSWCFTPE GIYFKLVSAQ NGDFPRWGDY
460 470 480 490 500
EDKRDLNYIR SLIRTGGWFD GTQQEYPPAD PSEHMYAPKY LLKNYDQFRY
510 520 530
LLENPYREPK STVEGGRRNQ GSDGRSSAVR GKLDTTEG
Length:538
Mass (Da):62,022
Last modified:March 15, 2005 - v2
Checksum:i9DCBE619284D7438
GO

Sequence cautioni

The sequence BAA01625 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10852 mRNA Translation: BAA01625.1 Different initiation.
PIRiA43415
RefSeqiNP_062112.2, NM_019239.2
XP_006242134.1, XM_006242072.2
XP_006242135.1, XM_006242073.3
XP_006242136.1, XM_006242074.3
XP_017450192.1, XM_017594703.1
UniGeneiRn.9803

Genome annotation databases

EnsembliENSRNOT00000023434; ENSRNOP00000023434; ENSRNOG00000017434
GeneIDi29582
KEGGirno:29582
UCSCiRGD:3084 rat

Similar proteinsi

Entry informationi

Entry nameiMGAT3_RAT
AccessioniPrimary (citable) accession number: Q02527
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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