UniProtKB - Q02522 (HGPRT_LACLA)
Protein
Hypoxanthine-guanine phosphoribosyltransferase
Gene
hpt
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Functioni
Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to the N9 position of the 6-oxopurines hypoxanthine and guanine to form the corresponding ribonucleotides IMP (inosine 5'-monophosphate) and GMP (guanosine 5'-monophosphate), with the release of PPi.By similarity
Catalytic activityi
- EC:2.4.2.8By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:2.4.2.8By similarityThis reaction proceeds in the backwardBy similarity direction.
Cofactori
Mg2+By similarity
: IMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.By similarityProteins known to be involved in this subpathway in this organism are:
- Hypoxanthine phosphoribosyltransferase (hprT), Hypoxanthine-guanine phosphoribosyltransferase (hpt)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.
Pathwayi: GMP biosynthesis via salvage pathway
This protein is involved in step 1 of the subpathway that synthesizes GMP from guanine.By similarityProteins known to be involved in this subpathway in this organism are:
- Hypoxanthine phosphoribosyltransferase (hprT), Hypoxanthine-guanine phosphoribosyltransferase (hpt)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from guanine, the pathway GMP biosynthesis via salvage pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 103 | MagnesiumBy similarity | 1 | |
Metal bindingi | 104 | MagnesiumBy similarity | 1 | |
Active sitei | 107 | Proton acceptorBy similarity | 1 | |
Binding sitei | 134 | GMPBy similarity | 1 | |
Binding sitei | 162 | GMP; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 168 | DiphosphateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 155 – 156 | GMPBy similarity | 2 |
GO - Molecular functioni
- guanine phosphoribosyltransferase activity Source: UniProtKB-EC
- hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- GMP salvage Source: UniProtKB-UniPathway
- IMP salvage Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Ligand | Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | LLAC272623:L25115-MONOMER |
UniPathwayi | UPA00591;UER00648 UPA00909;UER00887 |
Names & Taxonomyi
Protein namesi | Recommended name: Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8By similarity)Short name: HGPRT Short name: HGPRTase |
Gene namesi | Name:hpt Ordered Locus Names:LL0020 ORF Names:L25115 |
Organismi | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) |
Taxonomic identifieri | 272623 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139604 | 1 – 183 | Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST | 183 |
Proteomic databases
PaxDbi | Q02522 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 47 – 48 | DiphosphateBy similarity | 2 |
Sequence similaritiesi
Belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG0634, Bacteria |
HOGENOMi | CLU_073615_0_1_9 |
OMAi | TMDWMAV |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
InterProi | View protein in InterPro IPR005904, Hxn_phspho_trans IPR000836, PRibTrfase_dom IPR029057, PRTase-like |
Pfami | View protein in Pfam PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR01203, HGPRTase, 1 hit |
PROSITEi | View protein in PROSITE PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02522-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEKNLDKAI EKVLVSEEEI IEKSKELGEI LTKEYEGKNP LVLGILRGSV
60 70 80 90 100
PFLAELIKHI DCHLETDFMT VSSYHGGTKS SGEVKLILDV DTAVKGRDIL
110 120 130 140 150
IVEDIIDTGR TLKYLKELLE HRGANVKIVT LLDKPEGRIV EIKPDYSGFT
160 170 180
IPNEFVVGFG LDYEENYRNL PYVGVLKPEV YNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67015 Genomic DNA Translation: CAA47404.1 X69123 Genomic DNA Translation: CAA48876.1 AE005176 Genomic DNA Translation: AAK04118.1 |
PIRi | D86627 S30100 |
RefSeqi | NP_266176.1, NC_002662.1 WP_010905038.1, NC_002662.1 |
Genome annotation databases
EnsemblBacteriai | AAK04118; AAK04118; L25115 |
KEGGi | lla:L25115 |
PATRICi | fig|272623.7.peg.22 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67015 Genomic DNA Translation: CAA47404.1 X69123 Genomic DNA Translation: CAA48876.1 AE005176 Genomic DNA Translation: AAK04118.1 |
PIRi | D86627 S30100 |
RefSeqi | NP_266176.1, NC_002662.1 WP_010905038.1, NC_002662.1 |
3D structure databases
SMRi | Q02522 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 272623.L25115 |
Proteomic databases
PaxDbi | Q02522 |
Genome annotation databases
EnsemblBacteriai | AAK04118; AAK04118; L25115 |
KEGGi | lla:L25115 |
PATRICi | fig|272623.7.peg.22 |
Phylogenomic databases
eggNOGi | COG0634, Bacteria |
HOGENOMi | CLU_073615_0_1_9 |
OMAi | TMDWMAV |
Enzyme and pathway databases
UniPathwayi | UPA00591;UER00648 UPA00909;UER00887 |
BioCyci | LLAC272623:L25115-MONOMER |
Family and domain databases
CDDi | cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
InterProi | View protein in InterPro IPR005904, Hxn_phspho_trans IPR000836, PRibTrfase_dom IPR029057, PRTase-like |
Pfami | View protein in Pfam PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit |
TIGRFAMsi | TIGR01203, HGPRTase, 1 hit |
PROSITEi | View protein in PROSITE PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HGPRT_LACLA | |
Accessioni | Q02522Primary (citable) accession number: Q02522 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families