UniProtKB - Q02516 (HAP5_YEAST)
Transcriptional activator HAP5
HAP5
Functioni
Acts a component of the CCAT-binding factor, which is a transcriptional activator and binds to the upstream activation site (UAS2) of the CYC1 gene and other genes involved in mitochondrial electron transport and activates their expression. Recognizes the sequence 5'-CCAAT-3'. HAP5 is essential for DNA-binding activity. It may be the linchpin that binds to the subunit association domains (SAD) of HAP2 and HAP3 to bring these proteins together.
Miscellaneous
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- protein heterodimerization activity Source: InterPro
GO - Biological processi
- carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
- regulation of carbohydrate metabolic process Source: ComplexPortal
- regulation of cellular respiration Source: SGD
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator HAP5 |
Gene namesi | Name:HAP5 Ordered Locus Names:YOR358W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005885, HAP5 |
VEuPathDBi | FungiDB:YOR358W |
Subcellular locationi
Nucleus
Nucleus
- CCAAT-binding factor complex Source: SGD
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218260 | 1 – 242 | Transcriptional activator HAP5Add BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 7 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q02516 |
PaxDbi | Q02516 |
PRIDEi | Q02516 |
PTM databases
iPTMneti | Q02516 |
Interactioni
Subunit structurei
Component of the CCAT-binding factor (CBF or HAP complex II), which consists of one copy each of HAP2, HAP3, HAP4 and HAP5. The assembly of the HAP2-HAP3-HAP5 heteromer (HAP complex I) occurs in a one-step pathway and its binding to DNA is a prerequisite for the association of HAP4.
2 PublicationsBinary interactionsi
Q02516
With | #Exp. | IntAct |
---|---|---|
HAP2 [P06774] | 7 | EBI-8165,EBI-8152 |
GO - Molecular functioni
- protein heterodimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 34743, 433 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-1363N |
IntActi | Q02516, 10 interactors |
MINTi | Q02516 |
STRINGi | 4932.YOR358W |
Miscellaneous databases
RNActi | Q02516, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 108 | DisorderedSequence analysisAdd BLAST | 108 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 53 | Polar residuesSequence analysisAdd BLAST | 53 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1657, Eukaryota |
GeneTreei | ENSGT00940000155689 |
HOGENOMi | CLU_1134110_0_0_1 |
InParanoidi | Q02516 |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR027170, HAP5_su IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 |
PANTHERi | PTHR10252:SF8, PTHR10252:SF8, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTDRNFSPQQ GQGPQESLPE GPQPSTMIQR EEMNMPRQYS EQQQLQENEG
60 70 80 90 100
EGENTRLPVS EEEFRMVQEL QAIQAGHDQA NLPPSGRGSL EGEDNGNSDG
110 120 130 140 150
ADGEMDEDDE EYDVFRNVGQ GLVGHYKEIM IRYWQELINE IESTNEPGSE
160 170 180 190 200
HQDDFKSHSL PFARIRKVMK TDEDVKMISA EAPIIFAKAC EIFITELTMR
210 220 230 240
AWCVAERNKR RTLQKADIAE ALQKSDMFDF LIDVVPRRPL PQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19932 Genomic DNA Translation: AAC49610.1 Z75266 Genomic DNA Translation: CAA99687.1 BK006948 Genomic DNA Translation: DAA11119.1 |
PIRi | S67270 |
RefSeqi | NP_015003.1, NM_001183778.1 |
Genome annotation databases
EnsemblFungii | YOR358W_mRNA; YOR358W; YOR358W |
GeneIDi | 854540 |
KEGGi | sce:YOR358W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19932 Genomic DNA Translation: AAC49610.1 Z75266 Genomic DNA Translation: CAA99687.1 BK006948 Genomic DNA Translation: DAA11119.1 |
PIRi | S67270 |
RefSeqi | NP_015003.1, NM_001183778.1 |
3D structure databases
AlphaFoldDBi | Q02516 |
SMRi | Q02516 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34743, 433 interactors |
ComplexPortali | CPX-1830, CCAAT-binding factor complex |
DIPi | DIP-1363N |
IntActi | Q02516, 10 interactors |
MINTi | Q02516 |
STRINGi | 4932.YOR358W |
PTM databases
iPTMneti | Q02516 |
Proteomic databases
MaxQBi | Q02516 |
PaxDbi | Q02516 |
PRIDEi | Q02516 |
Genome annotation databases
EnsemblFungii | YOR358W_mRNA; YOR358W; YOR358W |
GeneIDi | 854540 |
KEGGi | sce:YOR358W |
Organism-specific databases
SGDi | S000005885, HAP5 |
VEuPathDBi | FungiDB:YOR358W |
Phylogenomic databases
eggNOGi | KOG1657, Eukaryota |
GeneTreei | ENSGT00940000155689 |
HOGENOMi | CLU_1134110_0_0_1 |
InParanoidi | Q02516 |
Miscellaneous databases
PROi | PR:Q02516 |
RNActi | Q02516, protein |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR027170, HAP5_su IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 |
PANTHERi | PTHR10252:SF8, PTHR10252:SF8, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAP5_YEAST | |
Accessioni | Q02516Primary (citable) accession number: Q02516 Secondary accession number(s): D6W353, Q08827 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families