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Protein

Laccase

Gene
N/A
Organism
Trametes hirsuta (White-rot fungus) (Coriolus hirsutus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Most probably plays an important role in lignin degradation. Cleaves the C-C and C-O bonds of some phenolic lignin model compounds (such as O- and P-quinols, aminophenols and phenylenediamine).

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85Copper 1; type 2By similarity1
Metal bindingi87Copper 2; type 3By similarity1
Metal bindingi130Copper 2; type 3By similarity1
Metal bindingi132Copper 3; type 3By similarity1
Metal bindingi416Copper 4; type 1By similarity1
Metal bindingi419Copper 1; type 2By similarity1
Metal bindingi421Copper 3; type 3By similarity1
Metal bindingi473Copper 3; type 3By similarity1
Metal bindingi474Copper 4; type 1By similarity1
Metal bindingi475Copper 2; type 3By similarity1
Metal bindingi479Copper 4; type 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLignin degradation
LigandCopper, Metal-binding

Enzyme and pathway databases

BRENDAi1.10.3.2 1623

Protein family/group databases

CAZyiAA1 Auxiliary Activities 1

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase
Diphenol oxidase
Ligninolytic phenoloxidase
Urishiol oxidase
OrganismiTrametes hirsuta (White-rot fungus) (Coriolus hirsutus)
Taxonomic identifieri5327 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeTrametes

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000000293922 – 520LaccaseAdd BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi106 ↔ 509Sequence analysis
Disulfide bondi138 ↔ 226Sequence analysis
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi229N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi354N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ02497
SMRiQ02497
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 148Plastocyanin-like 1Add BLAST126
Domaini160 – 302Plastocyanin-like 2Add BLAST143
Domaini369 – 491Plastocyanin-like 3Add BLAST123

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420, 3 hits
InterProiView protein in InterPro
IPR001117 Cu-oxidase
IPR011706 Cu-oxidase_2
IPR011707 Cu-oxidase_3
IPR033138 Cu_oxidase_CS
IPR008972 Cupredoxin
PfamiView protein in Pfam
PF00394 Cu-oxidase, 1 hit
PF07731 Cu-oxidase_2, 1 hit
PF07732 Cu-oxidase_3, 1 hit
SUPFAMiSSF49503 SSF49503, 3 hits
PROSITEiView protein in PROSITE
PS00079 MULTICOPPER_OXIDASE1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02497-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRFQSLLAF VVASLAAVAH AAIGPTADLT ISNAEVSPDG FARQAVVVNN
60 70 80 90 100
VTPGPLVAGN KGDRFQLNVI DNLTNHTMLK STSIHWHGFF QKGTNWADGP
110 120 130 140 150
AFVNQCPISS GHSFLYDFQV PDQAGTFWYH SHLSTQYCDG LRGPFVVYDP
160 170 180 190 200
NDPHASLYDV DNDDTVITLA DWYHTAAKLG PAFPLGADAT LINGLGRSPS
210 220 230 240 250
TTAADLAVIN VTKGKRYRFR LVSLSCDPNH TFSIDGHDLT IIEVDSINSQ
260 270 280 290 300
PLVVDSIQIF AAQRYSFVLN ADQDVGNYWI RANPNFGNVG FAGGINSAIL
310 320 330 340 350
RYDGADPVEP TTTQTTPTKP LNEVDLHPLA TMAVPGSPVA GGVDTAINMA
360 370 380 390 400
FNFNGTNFFI NGASFVPPTV PVLLQIISGA QNAQDLLPSG SVYSLPSNAD
410 420 430 440 450
IEISFPATAA APGAPHPFHL HGHAFAVVRS AGSTVYNYDN PIFRDVVSTG
460 470 480 490 500
TPAAGDNVTI RFRTDNPGPW FLHCHIDFHL EAGFAVVFAE DIPDVASANP
510 520
VPQAWSDLCP IYDALDVNDQ
Length:520
Mass (Da):55,688
Last modified:October 1, 1996 - v1
Checksum:i977D8DFA551F7929
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti378 – 379SG → RR in AAA33104 (PubMed:2394718).Curated2

Polymorphismi

2 allelic forms exist in position 111.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti411A → P1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60560 Genomic DNA Translation: AAA33103.1
M60561 Genomic DNA Translation: AAA33104.1
PIRiA35883

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60560 Genomic DNA Translation: AAA33103.1
M60561 Genomic DNA Translation: AAA33104.1
PIRiA35883

3D structure databases

ProteinModelPortaliQ02497
SMRiQ02497
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA1 Auxiliary Activities 1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.10.3.2 1623

Family and domain databases

Gene3Di2.60.40.420, 3 hits
InterProiView protein in InterPro
IPR001117 Cu-oxidase
IPR011706 Cu-oxidase_2
IPR011707 Cu-oxidase_3
IPR033138 Cu_oxidase_CS
IPR008972 Cupredoxin
PfamiView protein in Pfam
PF00394 Cu-oxidase, 1 hit
PF07731 Cu-oxidase_2, 1 hit
PF07732 Cu-oxidase_3, 1 hit
SUPFAMiSSF49503 SSF49503, 3 hits
PROSITEiView protein in PROSITE
PS00079 MULTICOPPER_OXIDASE1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLAC1_TRAHI
AccessioniPrimary (citable) accession number: Q02497
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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