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UniProtKB - Q02471 (PA2B4_DABSI)
Protein
Basic phospholipase A2 RV-4
Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Functioni
Heterodimer RV-4/RV-7: acts as a presynaptic neurotoxin.
Monomer: snake venom phospholipase A2 (PLA2) that acts as a presynaptic neurotoxin. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 63 | By similarity | 1 | |
Metal bindingi | 64 | CalciumBy similarity | 1 | |
Active sitei | 105 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Neurotoxin, Presynaptic neurotoxin, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Basic phospholipase A2 RV-4 (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): F4 Phosphatidylcholine 2-acylhydrolase S2 Viperotoxin F Viperotoxin toxic basic component |
Organismi | Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis) |
Taxonomic identifieri | 343250 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Viperinae › Daboia |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
LD50 is 0.32 mg/kg by intravenous injection for RV-4 and 0.15 mg/kg for RV-4/RV-7 complex.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | 2 PublicationsAdd BLAST | 16 | |
ChainiPRO_0000022977 | 17 – 138 | Basic phospholipase A2 RV-4Add BLAST | 122 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 131 | 1 Publication | ||
Disulfide bondi | 44 ↔ 60 | 1 Publication | ||
Disulfide bondi | 59 ↔ 111 | 1 Publication | ||
Disulfide bondi | 65 ↔ 138 | 1 Publication | ||
Disulfide bondi | 66 ↔ 104 | 1 Publication | ||
Disulfide bondi | 73 ↔ 97 | 1 Publication | ||
Disulfide bondi | 91 ↔ 102 | 1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Heterodimer of a weakly toxic basic protein having phospholipase A2 activity (RV-4) and a non-toxic acidic protein which inhibits its enzymatic activity but potentiates its lethal potency and neurotoxicity (RV-7).
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02471 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02471 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q02471-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLWIVAVC LIGVEGNLFQ FARMINGKLG AFSVWNYISY GCYCGWGGQG
60 70 80 90 100
TPKDATDRCC FVHDCCYGGV KGCNPKLAIY SYSFQRGNIV CGRNNGCLRT
110 120 130
ICECDRVAAN CFHQNKNTYN KEYKFLSSSK CRQRSEQC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68385 mRNA Translation: CAA48456.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68385 mRNA Translation: CAA48456.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OQS | X-ray | 1.90 | B/D/F/H | 17-138 | [»] | |
SMRi | Q02471 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02471 |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2B4_DABSI | |
Accessioni | Q02471Primary (citable) accession number: Q02471 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families