UniProtKB - Q02457 (TBF1_YEAST)
Protein
Protein TBF1
Gene
TBF1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of gene expression. 52 binding sites have been identified, distributed over 15 chromosomes. A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromatin to a promoter.4 Publications
Miscellaneous
Present with 6380 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 431 – 456 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 26 |
GO - Molecular functioni
- chromatin insulator sequence binding Source: SGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: SGD
- double-stranded telomeric DNA binding Source: GO_Central
- protein homodimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: SGD
- telomeric DNA binding Source: SGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- negative regulation of chromatin silencing Source: SGD
- negative regulation of telomere maintenance via telomerase Source: SGD
- snoRNA transcription by RNA polymerase II Source: SGD
- telomere maintenance via telomere lengthening Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein TBF1Alternative name(s): TBF-alpha TTAGGG repeat-binding factor 1 |
Gene namesi | Name:TBF1 Ordered Locus Names:YPL128C ORF Names:LPI16C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006049, TBF1 |
VEuPathDBi | FungiDB:YPL128C |
Subcellular locationi
Nucleus
- nuclear chromosome, telomeric region Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
Chromosome, Nucleus, TelomerePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197125 | 1 – 562 | Protein TBF1Add BLAST | 562 |
Proteomic databases
MaxQBi | Q02457 |
PaxDbi | Q02457 |
PRIDEi | Q02457 |
PTM databases
iPTMneti | Q02457 |
Interactioni
Subunit structurei
Homodimer.
CuratedBinary interactionsi
Q02457
With | #Exp. | IntAct |
---|---|---|
VID22 [Q05934] | 2 | EBI-19005,EBI-30350 |
GO - Molecular functioni
- protein homodimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 36053, 298 interactors |
DIPi | DIP-1918N |
IntActi | Q02457, 23 interactors |
MINTi | Q02457 |
STRINGi | 4932.YPL128C |
Miscellaneous databases
RNActi | Q02457, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 404 – 460 | HTH myb-typePROSITE-ProRule annotationAdd BLAST | 57 |
Phylogenomic databases
eggNOGi | ENOG502QRT9, Eukaryota |
HOGENOMi | CLU_008791_4_0_1 |
InParanoidi | Q02457 |
OMAi | LELKGPH |
Family and domain databases
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb IPR013867, Telomere_rpt-bd_fac_dimer_dom |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF08558, TRF, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02457-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSQVPNNNE SLNRFNDIIQ SLPARTRLTI CSLCLLDNIS TQLLRFLILN
60 70 80 90 100
ANSPNIIAVL TDQTAFLSSG ETEIFQTLVK LFKQIRMIYH TRSPLLSVHD
110 120 130 140 150
VAPGLWFPNS PPPLILRGHE AFIITAIRKA NLLTFLLTSL NCLNYGFELL
160 170 180 190 200
QSIFLDIFCP NTNTVGNNSL EQSGKFLKSQ AILYLDLKTQ AYIAGLKEFQ
210 220 230 240 250
DETNEISLEK KQELLDLIFP SNLADILVQR RTGDSGDITL LTPSEKDFVE
260 270 280 290 300
RCDRRRENLK IVQDFNSLTQ SYEWAQFIRE LLDYCNKNMG LIIWGRKGRG
310 320 330 340 350
KSPLYDFDVN EFDPQVLFST GTRTVEFMDD QNQPSSASAF LSTARPNHYS
360 370 380 390 400
THTPTTDVSS KNPAITQSIV DAAVAASMSN SSSGPHSSHN NSSNSNNNGS
410 420 430 440 450
IGLRKPKAKR TWSKEEEEAL VEGLKEVGPS WSKILDLYGP GGKITENLKN
460 470 480 490 500
RTQVQLKDKA RNWKLQYLKS GKPLPDYLIK VTGNLEKIYK AKKKFSQSPN
510 520 530 540 550
SSTIMEQNLS QHPSSAASAT EDTQTHQEDS HGQNSDNMPS NGLFGNSTSD
560
NTGFDPHLED GM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 71 | E → R in CAA49191 (PubMed:8423796).Curated | 1 | |
Sequence conflicti | 371 – 373 | DAA → ERR in CAA49191 (PubMed:8423796).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69394 Genomic DNA Translation: CAA49191.1 U43703 Genomic DNA Translation: AAB68230.1 BK006949 Genomic DNA Translation: DAA11305.1 |
PIRi | S69055 |
RefSeqi | NP_015197.1, NM_001183942.1 |
Genome annotation databases
EnsemblFungii | YPL128C_mRNA; YPL128C; YPL128C |
GeneIDi | 855975 |
KEGGi | sce:YPL128C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69394 Genomic DNA Translation: CAA49191.1 U43703 Genomic DNA Translation: AAB68230.1 BK006949 Genomic DNA Translation: DAA11305.1 |
PIRi | S69055 |
RefSeqi | NP_015197.1, NM_001183942.1 |
3D structure databases
SMRi | Q02457 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36053, 298 interactors |
DIPi | DIP-1918N |
IntActi | Q02457, 23 interactors |
MINTi | Q02457 |
STRINGi | 4932.YPL128C |
PTM databases
iPTMneti | Q02457 |
Proteomic databases
MaxQBi | Q02457 |
PaxDbi | Q02457 |
PRIDEi | Q02457 |
Genome annotation databases
EnsemblFungii | YPL128C_mRNA; YPL128C; YPL128C |
GeneIDi | 855975 |
KEGGi | sce:YPL128C |
Organism-specific databases
SGDi | S000006049, TBF1 |
VEuPathDBi | FungiDB:YPL128C |
Phylogenomic databases
eggNOGi | ENOG502QRT9, Eukaryota |
HOGENOMi | CLU_008791_4_0_1 |
InParanoidi | Q02457 |
OMAi | LELKGPH |
Miscellaneous databases
PROi | PR:Q02457 |
RNActi | Q02457, protein |
Family and domain databases
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb IPR013867, Telomere_rpt-bd_fac_dimer_dom |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF08558, TRF, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBF1_YEAST | |
Accessioni | Q02457Primary (citable) accession number: Q02457 Secondary accession number(s): D6W3N9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names