ID2

Functionⁱ

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.1 Publication

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from sequence modelⁱ
- 19274049
ion channel binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 16311606
protein dimerization activity Source: InterPro
RNA polymerase II transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adipose tissue development Source: Ensembl
adult locomotory behavior Source: Ensembl
bundle of His development Source: Ensembl
cell maturation Source: Ensembl
cell morphogenesis involved in neuron differentiation Source: Ensembl
cellular response to lithium ion Source: Ensembl
cellular senescence Source: UniProtKB
circadian regulation of gene expression Source: UniProtKB
circadian rhythm
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
embryonic digestive tract morphogenesis Source: UniProtKB
endodermal digestive tract morphogenesis Source: UniProtKB
entrainment of circadian clock by photoperiod Source: UniProtKB
enucleate erythrocyte differentiation Source: Ensembl
epithelial cell differentiation involved in mammary gland alveolus development Source: UniProtKB
locomotor rhythm Source: UniProtKB
mammary gland alveolus development Source: UniProtKB
mammary gland epithelial cell proliferation Source: UniProtKB
membranous septum morphogenesis Source: Ensembl
metanephros development Source: Ensembl
multicellular organism development
Source: ProtInc
Traceable author statementⁱ
- 1741406
natural killer cell differentiation Source: Ensembl
negative regulation of B cell differentiation Source: Ensembl
negative regulation of DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
negative regulation of DNA-binding transcription factor activity
Source: GDB
Inferred from direct assayⁱ
- 14627819
negative regulation of gene expression Source: UniProtKB
negative regulation of neural precursor cell proliferation Source: UniProtKB
negative regulation of neuron differentiation Source: UniProtKB
negative regulation of oligodendrocyte differentiation Source: Ensembl
negative regulation of osteoblast differentiation Source: Ensembl
negative regulation of transcription, DNA-templated
Source: GDB
Inferred from direct assayⁱ
- 14627819
negative regulation of transcription by RNA polymerase II Source: Ensembl
neuron differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
neuron fate commitment Source: UniProtKB
olfactory bulb development Source: Ensembl
oligodendrocyte development Source: Ensembl
Peyer's patch development Source: Ensembl
positive regulation of astrocyte differentiation Source: Ensembl
positive regulation of blood pressure Source: UniProtKB
positive regulation of cell cycle arrest Source: UniProtKB
positive regulation of erythrocyte differentiation Source: Ensembl
positive regulation of fat cell differentiation Source: Ensembl
positive regulation of gene expression Source: UniProtKB
positive regulation of macrophage differentiation Source: Ensembl
positive regulation of smooth muscle cell proliferation Source: UniProtKB
positive regulation of transcription, DNA-templated Source: UniProtKB
positive regulation of transcription involved in G1/S transition of mitotic cell cycle
Source: BHF-UCL
Inferred by curatorⁱ
- 16311606
regulation of cell cycle
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of circadian rhythm Source: UniProtKB
regulation of G1/S transition of mitotic cell cycle
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 16311606
regulation of lipid metabolic process Source: UniProtKB
thigmotaxis Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Repressor
Biological process	Biological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORⁱ	Q02363

SIGNORⁱ

Q02363

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA-binding protein inhibitor ID-2 Alternative name(s): Class B basic helix-loop-helix protein 26 Short name: bHLHb26 Inhibitor of DNA binding 2 Inhibitor of differentiation 2
Gene namesⁱ	Name:ID2 Synonyms:BHLHB26
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000115738.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:5361 ID2
MIMⁱ	600386 gene
neXtProtⁱ	NX_Q02363

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	3398
Open Targets More...OpenTargetsⁱ	ENSG00000115738
PharmGKBⁱ	PA29609

Polymorphism and mutation databases

BioMutaⁱ	ID2
DMDMⁱ	729806

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127240	1 – 134	DNA-binding protein inhibitor ID-2Add BLAST		134

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	14	PhosphoserineCombined sources		1
Modified residueⁱ	25	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q02363
PaxDbⁱ	Q02363
PeptideAtlas More...PeptideAtlasⁱ	Q02363
PRIDEⁱ	Q02363
ProteomicsDBⁱ	58083

PTM databases

iPTMnetⁱ	Q02363
PhosphoSitePlusⁱ	Q02363

Expressionⁱ

Tissue specificityⁱ

Highly expressed in early fetal tissues, including those of the central nervous system.

Developmental stageⁱ

Found in most early fetal tissues but not in the corresponding mature tissues.

Gene expression databases

Bgeeⁱ	ENSG00000115738 Expressed in 227 organ(s), highest expression level in adrenal gland
CleanExⁱ	HS_ID2
ExpressionAtlasⁱ	Q02363 baseline and differential
Genevisibleⁱ	Q02363 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA027612

HPAⁱ

HPA027612

Interactionⁱ

Subunit structureⁱ

Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5 (By similarity). Interacts with NR0B2. Interacts with CLOCK and ARNTL/BMAL1.By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CCNDBP1	O95273	4	EBI-713450,EBI-748961
PDLIM5	Q96HC4	5	EBI-713450,EBI-751267
PKD2	Q13563	7	EBI-713450,EBI-7813714
TCF12	Q99081	2	EBI-713450,EBI-722877
TCF3	P15923-1	3	EBI-713450,EBI-769645

GO - Molecular functionⁱ

ion channel binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 16311606
protein dimerization activity Source: InterPro
RNA polymerase II transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	109624, 77 interactors
Database of interacting proteins More...DIPⁱ	DIP-46874N
ELMⁱ	Q02363
IntActⁱ	Q02363, 29 interactors
Molecular INTeraction database More...MINTⁱ	Q02363
STRINGⁱ	9606.ENSP00000234091

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	32 – 49	Combined sources	18
Beta strandⁱ	55 – 57	Combined sources	3
Helixⁱ	61 – 80	Combined sources	20

Show more details

3D structure databases

ProteinModelPortalⁱ	Q02363
SMRⁱ	Q02363
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	23 – 75	bHLHPROSITE-ProRule annotationAdd BLAST		53

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	106 – 115	Nuclear export signalBy similarity		10

Domainⁱ

The bHLH domain is essential for its repressor activity towards the CLOCK-ARNTL/BMAL1 heterodimer.By similarity

Phylogenomic databases

eggNOGⁱ	ENOG410IVXR Eukaryota ENOG4111PGK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000000908
HOGENOMⁱ	HOG000234788
HOVERGENⁱ	HBG009009
InParanoidⁱ	Q02363
KEGG Orthology (KO) More...KOⁱ	K17693
OMAⁱ	FPSEFMS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0VHR
PhylomeDBⁱ	Q02363
TreeFamⁱ	TF326217

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR026052 DNA-bd_prot-inh IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR11723 PTHR11723, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q02363-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV 
        60         70         80         90        100
PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR 
       110        120        130 
TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG

Length:134

Mass (Da):14,917

Last modified:February 1, 1995 - v1

Checksum:ⁱA0D98B96396EB11E

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	68	V → L in AAA58681 (PubMed:1741406).Curated		1
Sequence conflictⁱ	98	A → R in AAA58681 (PubMed:1741406).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13891 mRNA Translation: BAA02990.1 M97796 mRNA Translation: AAA58681.1 BC030639 mRNA Translation: AAH30639.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS1659.1
PIRⁱ	A40227 JC2007
NCBI Reference Sequences More...RefSeqⁱ	NP_002157.2, NM_002166.4
UniGeneⁱ	Hs.180919 Hs.726053

Genome annotation databases

Ensemblⁱ	ENST00000234091; ENSP00000234091; ENSG00000115738 ENST00000331129; ENSP00000385465; ENSG00000115738 ENST00000396290; ENSP00000379585; ENSG00000115738
GeneIDⁱ	3398
KEGGⁱ	hsa:3398

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q02363	DNA-binding protein inhibitor ID-2		MACFA		134	UniRef100_Q02363
DNA-binding protein inhibitor ID-2		PONAB		134
Cell growth-inhibiting gene 8		HUMAN		134
ID2 isoform 1		PANTR		134
DNA-binding protein inhibitor ID-2		MACMU		134
+15

Protein	Similar proteins		Species	Score	Length	Source
Q02363	DNA-binding protein inhibitor ID-2		MACFA		134	UniRef90_Q02363
DNA-binding protein inhibitor ID-2		PONAB		134
Cell growth-inhibiting gene 8		HUMAN		134
ID2 isoform 1		PANTR		134
DNA-binding protein inhibitor ID-2		MACMU		134
+26

Protein	Similar proteins		Species	Score	Length	Source
Q02363	DNA-binding protein inhibitor ID-2		MACFA		134	UniRef50_Q02363
DNA-binding protein inhibitor ID-2		PONAB		134
Cell growth-inhibiting gene 8		HUMAN		134
ID2 isoform 1		PANTR		134
DNA-binding protein inhibitor ID-2		MACMU		134
+34

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13891 mRNA Translation: BAA02990.1 M97796 mRNA Translation: AAA58681.1 BC030639 mRNA Translation: AAH30639.1
CCDSⁱ	CCDS1659.1
PIRⁱ	A40227 JC2007
RefSeqⁱ	NP_002157.2, NM_002166.4
UniGeneⁱ	Hs.180919 Hs.726053

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4AYA	X-ray	2.10	A/B	1-82	[»]
ProteinModelPortalⁱ	Q02363
SMRⁱ	Q02363
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	109624, 77 interactors
DIPⁱ	DIP-46874N
ELMⁱ	Q02363
IntActⁱ	Q02363, 29 interactors
MINTⁱ	Q02363
STRINGⁱ	9606.ENSP00000234091

PTM databases

iPTMnetⁱ	Q02363
PhosphoSitePlusⁱ	Q02363

Polymorphism and mutation databases

BioMutaⁱ	ID2
DMDMⁱ	729806

Proteomic databases

EPDⁱ	Q02363
PaxDbⁱ	Q02363
PeptideAtlasⁱ	Q02363
PRIDEⁱ	Q02363
ProteomicsDBⁱ	58083

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	3398
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000234091; ENSP00000234091; ENSG00000115738 ENST00000331129; ENSP00000385465; ENSG00000115738 ENST00000396290; ENSP00000379585; ENSG00000115738
GeneIDⁱ	3398
KEGGⁱ	hsa:3398

Organism-specific databases

CTDⁱ	3398
DisGeNETⁱ	3398
EuPathDBⁱ	HostDB:ENSG00000115738.9
GeneCardsⁱ	ID2
H-InvDBⁱ	HIX0204445
HGNCⁱ	HGNC:5361 ID2
HPAⁱ	HPA027612
MIMⁱ	600386 gene
neXtProtⁱ	NX_Q02363
OpenTargetsⁱ	ENSG00000115738
PharmGKBⁱ	PA29609
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IVXR Eukaryota ENOG4111PGK LUCA
GeneTreeⁱ	ENSGT00390000000908
HOGENOMⁱ	HOG000234788
HOVERGENⁱ	HBG009009
InParanoidⁱ	Q02363
KOⁱ	K17693
OMAⁱ	FPSEFMS
OrthoDBⁱ	EOG091G0VHR
PhylomeDBⁱ	Q02363
TreeFamⁱ	TF326217

Enzyme and pathway databases

SIGNORⁱ	Q02363

SIGNORⁱ

Q02363

Miscellaneous databases

GeneWikiⁱ	ID2
GenomeRNAiⁱ	3398
Protein Ontology More...PROⁱ	PR:Q02363
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000115738 Expressed in 227 organ(s), highest expression level in adrenal gland
CleanExⁱ	HS_ID2
ExpressionAtlasⁱ	Q02363 baseline and differential
Genevisibleⁱ	Q02363 HS

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR026052 DNA-bd_prot-inh IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR11723 PTHR11723, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ID2_HUMAN
Accessionⁱ	Q02363Primary (citable) accession number: Q02363
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
	Last sequence update:	February 1, 1995
	Last modified:	November 7, 2018
	This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q02363 (ID2_HUMAN)

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DNA-binding protein inhibitor ID-2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ