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Protein

Transcriptional adapter 2

Gene

ADA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B.1 Publication

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 48ZZ-typePROSITE-ProRule annotationAdd BLAST48

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: InterPro
  • DNA binding transcription factor activity Source: GO_Central
  • phosphatidylserine binding Source: SGD
  • transcription coactivator activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • chromatin organization Source: SGD
  • chromatin remodeling Source: GO_Central
  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at telomere Source: SGD
  • histone acetylation Source: GOC
  • positive regulation of histone acetylation Source: SGD
  • regulation of transcription by RNA polymerase II Source: SGD
  • replication-born double-strand break repair via sister chromatid exchange Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29979-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional adapter 2
Gene namesi
Name:ADA2
Ordered Locus Names:YDR448W
ORF Names:D9461.33
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR448W
SGDiS000002856 ADA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001970821 – 434Transcriptional adapter 2Add BLAST434

Proteomic databases

MaxQBiQ02336
PaxDbiQ02336
PRIDEiQ02336

PTM databases

iPTMnetiQ02336

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least of TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9. Component of the ADA/GCN5 complex that consists of HFI1/ADA1, ADA2, ADA3, SPT20/ADA5 and GCN5 and is probably a subcomplex of SAGA. Component of the 0.8 MDa ADA complex, which at least consists of ADA2, ADA3, AHC1 and GCN5. ADA2 interacts with GCN5.8 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32503, 302 interactors
ComplexPortaliCPX-608 Ada2/Gcn5/Ada3 transcription activator complex
CPX-656 SAGA complex
CPX-675 SLIK (SAGA-like) complex
DIPiDIP-183N
IntActiQ02336, 169 interactors
MINTiQ02336
STRINGi4932.YDR448W

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi358 – 361Combined sources4
Beta strandi367 – 369Combined sources3
Helixi371 – 379Combined sources9
Helixi384 – 401Combined sources18
Helixi407 – 413Combined sources7
Helixi418 – 430Combined sources13

3D structure databases

ProteinModelPortaliQ02336
SMRiQ02336
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02336

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 112SANTPROSITE-ProRule annotationAdd BLAST53
Domaini349 – 434SWIRMPROSITE-ProRule annotationAdd BLAST86

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 48ZZ-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063657
HOGENOMiHOG000163455
InParanoidiQ02336
KOiK11314
OMAiCHEYDLC
OrthoDBiEOG092C1EX3

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR016827 Ada2/TADA2
IPR009057 Homeobox-like_sf
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR007526 SWIRM
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
PF00569 ZZ, 1 hit
PIRSFiPIRSF025024 Transcriptional_adaptor_2, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SM00291 ZnF_ZZ, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
PROSITEiView protein in PROSITE
PS51293 SANT, 1 hit
PS50934 SWIRM, 1 hit
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q02336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKFHCDVC SADCTNRVRV SCAICPEYDL CVPCFSQGSY TGKHRPYHDY
60 70 80 90 100
RIIETNSYPI LCPDWGADEE LQLIKGAQTL GLGNWQDIAD HIGSRGKEEV
110 120 130 140 150
KEHYLKYYLE SKYYPIPDIT QNIHVPQDEF LEQRRHRIES FRERPLEPPR
160 170 180 190 200
KPMASVPSCH EVQGFMPGRL EFETEFENEA EGPVKDMVFE PDDQPLDIEL
210 220 230 240 250
KFAILDIYNS RLTTRAEKKR LLFENHLMDY RKLQAIDKKR SKEAKELYNR
260 270 280 290 300
IKPFARVMTA QDFEEFSKDI LEELHCRARI QQLQEWRSNG LTTLEAGLKY
310 320 330 340 350
ERDKQARISS FEKFGASTAA SLSEGNSRYR SNSAHRSNAE YSQNYSENGG
360 370 380 390 400
RKKNMTISDI QHAPDYALLS NDEQQLCIQL KILPKPYLVL KEVMFRELLK
410 420 430
TGGNLSKSAC RELLNIDPIK ANRIYDFFQS QNWM
Length:434
Mass (Da):50,569
Last modified:July 1, 1993 - v1
Checksum:i9637E1EDBBED0AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95396 Genomic DNA Translation: AAA34393.1
U33007 Genomic DNA Translation: AAB64871.1
BK006938 Genomic DNA Translation: DAA12283.1
PIRiA43252
RefSeqiNP_010736.3, NM_001180756.3

Genome annotation databases

EnsemblFungiiYDR448W; YDR448W; YDR448W
GeneIDi852059
KEGGisce:YDR448W

Similar proteinsi

Entry informationi

Entry nameiADA2_YEAST
AccessioniPrimary (citable) accession number: Q02336
Secondary accession number(s): D6VT73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 18, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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