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Protein

Potassium voltage-gated channel protein eag

Gene

eag

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Structural component of a potassium channel. Mediates the potassium permeability of membranes; potassium current is regulated by CaMKII and CASK. Has a role in growth of the perineurial glial layer of the larval peripheral nerve.4 Publications

Miscellaneous

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The segment H5 is thought to line the channel pore.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi571 – 688cNMPAdd BLAST118

GO - Molecular functioni

  • phosphorelay sensor kinase activity Source: InterPro
  • voltage-gated cation channel activity Source: FlyBase
  • voltage-gated potassium channel activity Source: FlyBase

GO - Biological processi

  • behavioral response to ether Source: FlyBase
  • courtship behavior Source: FlyBase
  • learning Source: FlyBase
  • learning or memory Source: FlyBase
  • perineurial glial growth Source: FlyBase
  • potassium ion transport Source: FlyBase
  • protein complex oligomerization Source: FlyBase
  • regulation of heart contraction Source: FlyBase
  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • regulation of membrane potential Source: GO_Central
  • sensory perception of smell Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Ion channel, Potassium channel, Voltage-gated channel
Biological processDifferentiation, Ion transport, Neurogenesis, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-DME-1296072 Voltage gated Potassium channels

Protein family/group databases

TCDBi1.A.1.20.6 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel protein eag
Alternative name(s):
Ether-a-go-go protein
Gene namesi
Name:eag
ORF Names:CG10952
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000535 eag

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 226CytoplasmicSequence analysisAdd BLAST226
Transmembranei227 – 246Helical; Name=Segment S1Sequence analysisAdd BLAST20
Topological domaini247 – 268ExtracellularSequence analysisAdd BLAST22
Transmembranei269 – 291Helical; Name=Segment S2Sequence analysisAdd BLAST23
Topological domaini292 – 313CytoplasmicSequence analysisAdd BLAST22
Transmembranei314 – 335Helical; Name=Segment S3Sequence analysisAdd BLAST22
Topological domaini336 – 342ExtracellularSequence analysis7
Transmembranei343 – 369Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST27
Topological domaini370 – 371CytoplasmicSequence analysis2
Transmembranei372 – 393Helical; Name=Segment S5Sequence analysisAdd BLAST22
Topological domaini394 – 441ExtracellularSequence analysisAdd BLAST48
Intramembranei442 – 467Pore-forming; Name=Segment H5Sequence analysisAdd BLAST26
Topological domaini468 – 470ExtracellularSequence analysis3
Transmembranei471 – 493Helical; Name=Segment S6Sequence analysisAdd BLAST23
Topological domaini494 – 1174CytoplasmicSequence analysisAdd BLAST681

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Increased growth of the perineurial glial layer of the larval peripheral nerve. Hyperexcitability at the larval neuromuscular junction (NMJ) and memory formation defects in the adult.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi787T → A: Reduced eag channel amplitude and accelerated inactivation. Does not affect binding with CASK. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539641 – 1174Potassium voltage-gated channel protein eagAdd BLAST1174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi412N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei787Phosphothreonine; by CaMK21 Publication1

Post-translational modificationi

When in complex with CASK, the efficiency of Thr-787 phosphorylation is increased.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ02280
PRIDEiQ02280

PTM databases

iPTMnetiQ02280

Expressioni

Tissue specificityi

Expressed in the axon and the terminal boutons of motor neurons.1 Publication

Gene expression databases

BgeeiFBgn0000535
ExpressionAtlasiQ02280 baseline and differential
GenevisibleiQ02280 DM

Interactioni

Subunit structurei

The voltage-dependent potassium channel is a heterotetramer of potassium channel proteins (By similarity). Interaction with CASK.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CASKQ2421010EBI-85304,EBI-214423

Protein-protein interaction databases

BioGridi58786, 6 interactors
IntActiQ02280, 4 interactors
STRINGi7227.FBpp0110308

Structurei

Secondary structure

11174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi788 – 791Combined sources4

3D structure databases

ProteinModelPortaliQ02280
SMRiQ02280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 97PASPROSITE-ProRule annotationAdd BLAST55
Domaini113 – 165PACPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi453 – 458Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0501 Eukaryota
ENOG410XPSE LUCA
GeneTreeiENSGT00900000140833
InParanoidiQ02280
KOiK04908
OMAiDKAGETR
OrthoDBiEOG091G0OXR
PhylomeDBiQ02280

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
cd00130 PAS, 1 hit
Gene3Di1.20.120.350, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR003949 K_chnl_volt-dep_EAG
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR014710 RmlC-like_jellyroll
IPR027359 Volt_channel_dom_sf
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF13426 PAS_9, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
PR01464 EAGCHANNEL
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
SSF55785 SSF55785, 1 hit
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 1 hit
PS50113 PAC, 1 hit
PS50112 PAS, 1 hit

Sequencei

Sequence statusi: Complete.

Q02280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGGRRGLVA PQNTFLENII RRSNSQPDSS FLLANAQIVD FPIVYCNESF
60 70 80 90 100
CKISGYNRAE VMQKSCRYVC GFMYGELTDK ETVGRLEYTL ENQQQDQFEI
110 120 130 140 150
LLYKKNNLQC GCALSQFGKA QTQETPLWLL LQVAPIRNER DLVVLFLLTF
160 170 180 190 200
RDITALKQPI DSEDTKGVLG LSKFAKLARS VTRSRQFSAH LPTLKDPTKQ
210 220 230 240 250
SNLAHMMSLS ADIMPQYRQE APKTPPHILL HYCAFKAIWD WVILCLTFYT
260 270 280 290 300
AIMVPYNVAF KNKTSEDVSL LVVDSIVDVI FFIDIVLNFH TTFVGPGGEV
310 320 330 340 350
VSDPKVIRMN YLKSWFIIDL LSCLPYDVFN AFDRDEDGIG SLFSALKVVR
360 370 380 390 400
LLRLGRVVRK LDRYLEYGAA MLILLLCFYM LVAHWLACIW YSIGRSDADN
410 420 430 440 450
GIQYSWLWKL ANVTQSPYSY IWSNDTGPEL VNGPSRKSMY VTALYFTMTC
460 470 480 490 500
MTSVGFGNVA AETDNEKVFT ICMMIIAALL YATIFGHVTT IIQQMTSATA
510 520 530 540 550
KYHDMLNNVR EFMKLHEVPK ALSERVMDYV VSTWAMTKGL DTEKVLNYCP
560 570 580 590 600
KDMKADICVH LNRKVFNEHP AFRLASDGCL RALAMHFMMS HSAPGDLLYH
610 620 630 640 650
TGESIDSLCF IVTGSLEVIQ DDEVVAILGK GDVFGDQFWK DSAVGQSAAN
660 670 680 690 700
VRALTYCDLH AIKRDKLLEV LDFYSAFANS FARNLVLTYN LRHRLIFRKV
710 720 730 740 750
ADVKREKELA ERRKNEPQLP QNQDHLVRKI FSKFRRTPQV QAGSKELVGG
760 770 780 790 800
SGQSDVEKGD GEVERTKVFP KAPKLQASQA TLARQDTIDE GGEVDSSPPS
810 820 830 840 850
RDSRVVIEGA AVSSATVGPS PPVATTSSAA AGAGVSGGPG SGGTVVAIVT
860 870 880 890 900
KADRNLALER ERQIEMASSR ATTSDTYDTG LRETPPTLAQ RDLIATVLDM
910 920 930 940 950
KVDVRLELQR MQQRIGRIED LLGELVKRLA PGAGSGGNAP DNSSGQTTPG
960 970 980 990 1000
DEICAGCGAG GGGTPTTQAP PTSAVTSPVD TVITISSPGA SGSGSGTGAG
1010 1020 1030 1040 1050
AGSAVAGAGG AGLLNPGATV VSSAGGNGLG PLMLKKRRSK SRKAPAPPKQ
1060 1070 1080 1090 1100
TLASTAGTAT AAPAGVAGSG MTSSAPASAD QQQQHQSTAD QSPTTPGAEL
1110 1120 1130 1140 1150
LHLRLLEEDF TAAQLPSTSS GGAGGGGGSG SGATPTTPPP TTAGGSGSGT
1160 1170
PTSTTATTTP TGSGTATRGK LDFL
Length:1,174
Mass (Da):126,371
Last modified:June 21, 2005 - v2
Checksum:iB8F86ACBB5627FD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti548Y → C in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti567N → D in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti571A → T in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti699K → R in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti769F → L in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti894I → V in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti934G → S in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti1015N → D in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti1042R → G in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti1049K → E in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti1088T → A in AAA28495 (PubMed:1840699).Curated1
Sequence conflicti1142T → I in AAA28495 (PubMed:1840699).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61157 mRNA Translation: AAA28495.1
AE014298 Genomic DNA Translation: AAF48410.1
PIRiA40853
RefSeqiNP_511158.2, NM_078603.4
UniGeneiDm.2914

Genome annotation databases

EnsemblMetazoaiFBtr0073955; FBpp0073772; FBgn0000535
GeneIDi32428
KEGGidme:Dmel_CG10952

Similar proteinsi

Entry informationi

Entry nameiKCNAE_DROME
AccessioniPrimary (citable) accession number: Q02280
Secondary accession number(s): Q9VXZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 21, 2005
Last modified: July 18, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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