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UniProtKB - Q02251 (MCAS_MYCBO)
Protein
Mycocerosic acid synthase
Gene
mas
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Catalyzes the elongation of long-chain fatty acyl-CoA with 3 or 4 methylmalonyl-CoA (not malonyl-CoA) as the elongating agent to form tri- or tetramethylated-branched fatty acids known as mycocerosyl lipids.
1 PublicationCatalytic activityi
- 3 (R)-methylmalonyl-CoA + 9 H+ + long-chain fatty acyl-[mycocerosate synthase] + 6 NADPH = 3 CO2 + 3 CoA + 3 H2O + 6 NADP+ + trimethylated-mycocerosoyl-[mycocerosate synthase]1 PublicationEC:2.3.1.1111 Publication
- 4 (R)-methylmalonyl-CoA + 12 H+ + long-chain fatty acyl-[mycocerosate synthase] + 8 NADPH = 4 CO2 + 4 CoA + 4 H2O + 8 NADP+ + tetramethylated-mycocerosoyl-[mycocerosate synthase]1 PublicationEC:2.3.1.1111 Publication
Cofactori
pantetheine 4'-phosphate1 PublicationNote: Binds 1 phosphopantetheine covalently.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 177 | For beta-ketoacyl synthase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 623 | For acyltransferase activityPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1562 – 1579 | NADP; for enoyl reductase activityAdd BLAST | 18 | |
Nucleotide bindingi | 1766 – 1781 | NADP; for ketoreductase activityAdd BLAST | 16 |
GO - Molecular functioni
- 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: InterPro
- hydrolase activity Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
- mycocerosate synthase activity Source: UniProtKB-EC
- oxidoreductase activity Source: UniProtKB-KW
- phosphopantetheine binding Source: InterPro
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Ligase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mas Ordered Locus Names:BQ2027_MB2965C |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180298 | 1 – 2111 | Mycocerosic acid synthaseAdd BLAST | 2111 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2060 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinInteractioni
Subunit structurei
Homodimer whose monomers probably have a head to tail arrangement.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2025 – 2100 | CarrierPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 430 | Beta-ketoacyl synthaseAdd BLAST | 430 | |
Regioni | 533 – 852 | AcyltransferaseAdd BLAST | 320 |
Phylogenomic databases
OMAi | PDFEWAI |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.10.129.110, 1 hit 3.40.366.10, 1 hit 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR036291, NAD(P)-bd_dom_sf IPR032821, PKS_assoc IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020843, PKS_ER IPR013968, PKS_KR IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit PF16197, KAsynt_C_assoc, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF08659, KR, 1 hit PF00550, PP-binding, 1 hit PF14765, PS-DH, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00826, PKS_DH, 1 hit SM00829, PKS_ER, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 3 hits SSF52151, SSF52151, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02251-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESRVTPVAV IGMGCRLPGG INSPDKLWES LLRGDDLVTE IPPDRWDADD
60 70 80 90 100
YYDPEPGVPG RSVSRWGGFL DDVAGFDAEF FGISEREATS IDPQQRLLLE
110 120 130 140 150
TSWEAIEHAG LDPASLAGSS TAVFTGLTHE DYLVLTTTAG GLASPYVVTG
160 170 180 190 200
LNNSVASGRI AHTLGLHGPA MTFDTACSSG LMAVHLACRS LHDGEADLAL
210 220 230 240 250
AGGCAVLLEP HACVAASAQG MLSSTGRCHS FDADADGFVR SEGCAMVLLK
260 270 280 290 300
RLPDALRDGN RIFAVVRGTA TNQDGRTETL TMPSEDAQVA VYRAALAAAG
310 320 330 340 350
VQPETVGVVE AHGTGTPIGD PIEYRSLARV YGAGTPCALG SAKSNMGHST
360 370 380 390 400
ASAGTVGLIK AILSLRHGVV PPLLHFNRLP DELSDVETGL FVPQAVTPWP
410 420 430 440 450
NGNDHTPKRV AVSSFGMSGT NVHAIVEEAP AEASAPESSP GDAEVGPRLF
460 470 480 490 500
MLSSTSSDAL RQTARQLATW VEEHQDCVAA SDLAYTLARG RAHRPVRTAV
510 520 530 540 550
VAANLPELVE GLREVADGDA LYDAAVGHGD RGPVWVFSGQ GSQWAAMGTQ
560 570 580 590 600
LLASEPVFAA TIAKLEPVIA AESGFSVTEA ITAQQTVTGI DKVQPAVFAV
610 620 630 640 650
QVALAATMEQ TYGVRPGAVV GHSMGESAAA VVAGALSLED AARVICRRSK
660 670 680 690 700
LMTRIAGAGA MGSVELPAKQ VNSELMARGI DDVVVSVVAS PQSTVIGGTS
710 720 730 740 750
DTVRDLIARW EQRDVMAREV AVDVASHSPQ VDPILDDLAA ALADIAPMTP
760 770 780 790 800
KVPYYSATLF DPREQPVCDG AYWVDNLRNT VQFAAAVQAA MEDGYRVFAE
810 820 830 840 850
LSPHPLLTHA VEQTGRSLDM SVAALAGMRR EQPLPHGLRG LLTELHRAGA
860 870 880 890 900
ALDYSALYPA GRLVDAPLPA WTHARLFIDD DGQEQRAQGA CTITVHPLLG
910 920 930 940 950
SHVRLTEEPE RHVWQGDVGT SVLSWLSDHQ VHNVAALPGA AYCEMALAAA
960 970 980 990 1000
AEVFGEAAEV RDITFEQMLL LDEQTPIDAV ASIDAPGVVN FTVETNRDGE
1010 1020 1030 1040 1050
TTRHATAALR AAEDDCPPPG YDITALLQAH PHAVNGTAMR ESFAERGVTL
1060 1070 1080 1090 1100
GAAFGGLTTA HTAEAGAATV LAEVALPASI RFQQGAYRIH PALLDACFQS
1110 1120 1130 1140 1150
VGAGVQAGTA TGGLLLPLGV RSLRAYGPTR NARYCYTRLT KAFNDGTRGG
1160 1170 1180 1190 1200
EADLDVLDEH GTVLLAVRGL RMGTGTSERD ERDRLVSERL LTLGWQQRAL
1210 1220 1230 1240 1250
PEVGDGEAGS WLLIDTSNAV DTPDMLASTL TDALKSHGPQ GTECASLSWS
1260 1270 1280 1290 1300
VQDTPPNDQA GLEKLGSQLR GRDGVVIVYG PRVGDPDEHS LLAGREQVRH
1310 1320 1330 1340 1350
LVRITRELAE FEGELPRLFV VTRQAQIVKP HDSGERANLE QAGLRGLLRV
1360 1370 1380 1390 1400
ISSEHPMLRT TLIDVDEHTD VERVAQQLLS GSEEDETAWR NGDWYVARLT
1410 1420 1430 1440 1450
PSPLGHEERR TAVLDPDHDG MRVQVRRPGD LQTLEFVASD RVPPGPGQIE
1460 1470 1480 1490 1500
VAVSMSSINF ADVLIAFGRF PIIDDREPQL GMDFVGVVTA VGEGVTGHQV
1510 1520 1530 1540 1550
GDRVGGFSEG GCWRTFLTCD ANLAVTLPPG LTDEQAITAA TAHATAWYGL
1560 1570 1580 1590 1600
NDLAQIKAGD KVLIHSATGG VGQAAISIAR AKGAEIFATA GNPAKRAMLR
1610 1620 1630 1640 1650
DMGVEHVYDS RSVEFAEQIR RDTDGYGVDI VLNSLTGAAQ RAGLELLAFG
1660 1670 1680 1690 1700
GRFVEIGKAD VYGNTRLGLF PFRRGLTFYY LDLALMSVTQ PDRVRELLAT
1710 1720 1730 1740 1750
VFKLTADGVL TAPQCTHYPL AEAADAIRAM SNAEHTGKLV LDVPRSGRRS
1760 1770 1780 1790 1800
VAVTPEQAPL YRRDGSYIIT GGLGGLGLFF ASKLAAAGCG RIVLTARSQP
1810 1820 1830 1840 1850
NPKARQTIEG LRAAGADIVV ECGNIAEPDT ADRLVSAATA TGLPLRGVLH
1860 1870 1880 1890 1900
SAAVVEDATL TNITDELIDR DWSPKVFGSW NLHRATLGQP LDWFCLFSSG
1910 1920 1930 1940 1950
AALLGSPGQG AYAAANSWVD VFAHWRRAQG LPVSAIAWGA WGEVGRATFL
1960 1970 1980 1990 2000
AEGGEIMITP EEGAYAFETL VRHDRAYSGY IPILGAPWLA DLVRRSPWGE
2010 2020 2030 2040 2050
MFASTGQRSR GPSKFRMELL SLPQDEWAGR LRRLLVEQAS VILRRTIDAD
2060 2070 2080 2090 2100
RSFIEYGLDS LGMLEMRTHV ETETGIRLTP KVIATNNTAR ALAQYLADTL
2110
AEEQAAAPAA S
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 196 | A → R in AAA25369 (PubMed:1527058).Curated | 1 | |
Sequence conflicti | 492 | A → R in AAA25369 (PubMed:1527058).Curated | 1 | |
Sequence conflicti | 800 – 801 | EL → DV in AAA25369 (PubMed:1527058).Curated | 2 | |
Sequence conflicti | 1063 – 1150 | AEAGA…GTRGG → PRREPRRCWPRSRCPRRSGS SRAPTESTRRCWTLVSSRSA RASSRYGHWWPAVAVGCAQP ACLRAYPQCPLLLHAVDQGL QRRDPRW in AAA25369 (PubMed:1527058).CuratedAdd BLAST | 88 | |
Sequence conflicti | 1648 | A → P in AAA25369 (PubMed:1527058).Curated | 1 | |
Sequence conflicti | 1758 | A → R in AAA25369 (PubMed:1527058).Curated | 1 | |
Sequence conflicti | 1926 | R → P in AAA25369 (PubMed:1527058).Curated | 1 | |
Sequence conflicti | 2087 | N → K in AAA25369 (PubMed:1527058).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95808 Genomic DNA Translation: AAA25369.1 LT708304 Genomic DNA Translation: SIU01586.1 |
PIRi | B44110 |
RefSeqi | NP_856610.1, NC_002945.3 WP_010950802.1, NC_002945.4 |
Genome annotation databases
EnsemblBacteriai | SIU01586; SIU01586; BQ2027_MB2965C |
PATRICi | fig|233413.5.peg.3254 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95808 Genomic DNA Translation: AAA25369.1 LT708304 Genomic DNA Translation: SIU01586.1 |
PIRi | B44110 |
RefSeqi | NP_856610.1, NC_002945.3 WP_010950802.1, NC_002945.4 |
3D structure databases
SMRi | Q02251 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | SIU01586; SIU01586; BQ2027_MB2965C |
PATRICi | fig|233413.5.peg.3254 |
Phylogenomic databases
OMAi | PDFEWAI |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.10.129.110, 1 hit 3.40.366.10, 1 hit 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR036291, NAD(P)-bd_dom_sf IPR032821, PKS_assoc IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020843, PKS_ER IPR013968, PKS_KR IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit PF16197, KAsynt_C_assoc, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF08659, KR, 1 hit PF00550, PP-binding, 1 hit PF14765, PS-DH, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00826, PKS_DH, 1 hit SM00829, PKS_ER, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 3 hits SSF52151, SSF52151, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCAS_MYCBO | |
Accessioni | Q02251Primary (citable) accession number: Q02251 Secondary accession number(s): A0A1R3Y3F3, X2BLY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | July 11, 2003 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |