UniProtKB - Q02159 (UBC7_YEAST)
Protein
Ubiquitin-conjugating enzyme E2 7
Gene
UBC7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Involved in resistance to cadmium poisoning.PROSITE-ProRule annotation7 Publications
Miscellaneous
Present with 2100 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- chromatin assembly or disassembly Source: SGD
- fungal-type cell wall organization Source: SGD
- protein polyubiquitination Source: GO_Central
- response to cadmium ion Source: UniProtKB-KW
- ubiquitin-dependent ERAD pathway Source: SGD
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Cadmium resistance, Ubl conjugation pathway |
Ligand | ATP-binding, Cadmium, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 7 (EC:2.3.2.231 Publication)Alternative name(s): E2 ubiquitin-conjugating enzyme 7 Ubiquitin carrier protein Ubiquitin-conjugating enzyme E2-18 kDa Ubiquitin-protein ligase |
Gene namesi | Name:UBC7 Synonyms:QRI8 Ordered Locus Names:YMR022W ORF Names:YM9711.12 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004624, UBC7 |
VEuPathDBi | FungiDB:YMR022W |
Subcellular locationi
Endoplasmic reticulum
Note: Anchored via the membrane protein CUE1 to the endoplasmic reticulum membrane.
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- Doa10p ubiquitin ligase complex Source: SGD
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: SGD
- Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | C → S: No effect; when associated with S-141. 1 Publication | 1 | |
Mutagenesisi | 81 | N → A: Impairs degradation of UBC7-substrates, but does not prevent protein degradation. | 1 | |
Mutagenesisi | 89 | C → A or S: Prevents polyubiquitin chain attachment and protein degradation. 1 Publication | 1 | |
Mutagenesisi | 141 | C → S: No effect; when associated with S-39. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082554 | 1 – 165 | Ubiquitin-conjugating enzyme E2 7Add BLAST | 165 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 89 | Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Autoubiquitinated at Cys-89; undergoes 'Lys-48'-linked polyubiquitination, which leads to proteasome-dependent protein degradation. Degradation is autoregulated when its levels exceed that of its binding partner CUE1.1 Publication
Keywords - PTMi
Thioester bond, Ubl conjugationProteomic databases
MaxQBi | Q02159 |
PaxDbi | Q02159 |
PRIDEi | Q02159 |
PTM databases
iPTMneti | Q02159 |
Expressioni
Inductioni
By cadmium.
Interactioni
Subunit structurei
Forms a heterodimer with CUE1.
Interacts with SSM4/DOA10 and HDR1.
Interacts with UFD4.
5 PublicationsProtein-protein interaction databases
BioGRIDi | 35193, 249 interactors |
ComplexPortali | CPX-2948, CUE1-UBC7 ubiquitin-conjugating enzyme complex |
DIPi | DIP-6583N |
IntActi | Q02159, 619 interactors |
STRINGi | 4932.YMR022W |
Miscellaneous databases
RNActi | Q02159, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02159 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q02159 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 164 | UBC corePROSITE-ProRule annotationAdd BLAST | 161 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0426, Eukaryota |
GeneTreei | ENSGT00940000158193 |
HOGENOMi | CLU_030988_10_1_1 |
InParanoidi | Q02159 |
OMAi | PNMYESS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02159-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKTAQKRLL KELQQLIKDS PPGIVAGPKS ENNIFIWDCL IQGPPDTPYA
60 70 80 90 100
DGVFNAKLEF PKDYPLSPPK LTFTPSILHP NIYPNGEVCI SILHSPGDDP
110 120 130 140 150
NMYELAEERW SPVQSVEKIL LSVMSMLSEP NIESGANIDA CILWRDNRPE
160
FERQVKLSIL KSLGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66829 Genomic DNA Translation: CAA47302.1 X69100 Genomic DNA Translation: CAA48846.1 Z49211 Genomic DNA Translation: CAA89125.1 AY558116 Genomic DNA Translation: AAS56442.1 BK006946 Genomic DNA Translation: DAA09920.1 |
PIRi | S28951 |
RefSeqi | NP_013735.1, NM_001182518.1 |
Genome annotation databases
EnsemblFungii | YMR022W_mRNA; YMR022W; YMR022W |
GeneIDi | 855036 |
KEGGi | sce:YMR022W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66829 Genomic DNA Translation: CAA47302.1 X69100 Genomic DNA Translation: CAA48846.1 Z49211 Genomic DNA Translation: CAA89125.1 AY558116 Genomic DNA Translation: AAS56442.1 BK006946 Genomic DNA Translation: DAA09920.1 |
PIRi | S28951 |
RefSeqi | NP_013735.1, NM_001182518.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2UCZ | X-ray | 2.93 | A | 1-165 | [»] | |
4JQU | X-ray | 1.81 | A | 2-165 | [»] | |
SMRi | Q02159 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35193, 249 interactors |
ComplexPortali | CPX-2948, CUE1-UBC7 ubiquitin-conjugating enzyme complex |
DIPi | DIP-6583N |
IntActi | Q02159, 619 interactors |
STRINGi | 4932.YMR022W |
PTM databases
iPTMneti | Q02159 |
Proteomic databases
MaxQBi | Q02159 |
PaxDbi | Q02159 |
PRIDEi | Q02159 |
Genome annotation databases
EnsemblFungii | YMR022W_mRNA; YMR022W; YMR022W |
GeneIDi | 855036 |
KEGGi | sce:YMR022W |
Organism-specific databases
SGDi | S000004624, UBC7 |
VEuPathDBi | FungiDB:YMR022W |
Phylogenomic databases
eggNOGi | KOG0426, Eukaryota |
GeneTreei | ENSGT00940000158193 |
HOGENOMi | CLU_030988_10_1_1 |
InParanoidi | Q02159 |
OMAi | PNMYESS |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | Q02159 |
PROi | PR:Q02159 |
RNActi | Q02159, protein |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC7_YEAST | |
Accessioni | Q02159Primary (citable) accession number: Q02159 Secondary accession number(s): D6VZJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families