UniProtKB - Q02111 (KPCT_MOUSE)
Protein
Protein kinase C theta type
Gene
Prkcq
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylates CCDC88A/GIV and inhibits its guanine nucleotide exchange factor activity (By similarity).By similarity6 Publications
Catalytic activityi
Cofactori
Activity regulationi
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 409 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 504 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 159 – 209 | Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 231 – 281 | Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST | 51 | |
Nucleotide bindingi | 386 – 394 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein kinase C activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- protein kinase C activity Source: MGI
- protein serine/threonine kinase activity Source: MGI
GO - Biological processi
- cell chemotaxis Source: MGI
- immune system process Source: UniProtKB-KW
- inflammatory response Source: UniProtKB-KW
- intracellular signal transduction Source: MGI
- membrane protein ectodomain proteolysis Source: BHF-UCL
- negative regulation of insulin receptor signaling pathway Source: UniProtKB
- negative regulation of T cell apoptotic process Source: UniProtKB
- peptidyl-serine phosphorylation Source: GO_Central
- positive regulation of filopodium assembly Source: MGI
- positive regulation of interleukin-17 production Source: UniProtKB
- positive regulation of interleukin-2 production Source: MGI
- positive regulation of interleukin-4 production Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of protein import into nucleus Source: MGI
- positive regulation of protein secretion Source: MGI
- positive regulation of stress fiber assembly Source: MGI
- positive regulation of T cell activation Source: UniProtKB
- positive regulation of T cell proliferation Source: MGI
- positive regulation of telomerase activity Source: MGI
- positive regulation of telomere capping Source: MGI
- positive regulation of telomere maintenance via telomerase Source: MGI
- positive regulation of T-helper 17 type immune response Source: UniProtKB
- positive regulation of T-helper 2 cell activation Source: UniProtKB
- protein phosphorylation Source: MGI
- regulation of G2/M transition of mitotic cell cycle Source: MGI
- regulation of platelet aggregation Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Immunity, Inflammatory response |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-111465, Apoptotic cleavage of cellular proteins R-MMU-114508, Effects of PIP2 hydrolysis R-MMU-202424, Downstream TCR signaling R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-373752, Netrin-1 signaling R-MMU-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: Protein kinase C theta type (EC:2.7.11.13By similarity)Alternative name(s): nPKC-theta |
Gene namesi | Name:Prkcq Synonyms:Pkcq |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97601, Prkcq |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein
Other locations
- Cytoplasm By similarity
Note: In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse (By similarity).By similarity
Cytoskeleton
- centriolar satellite Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- immunological synapse Source: MGI
- plasma membrane Source: MGI
- sarcolemma Source: MGI
Other locations
- aggresome Source: MGI
- cytoplasm Source: MGI
- membrane Source: MGI
- neuromuscular junction Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055709 | 1 – 707 | Protein kinase C theta typeAdd BLAST | 707 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 90 | Phosphotyrosine; by LCKBy similarity | 1 | |
Modified residuei | 219 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 348 | PhosphoserineBy similarity | 1 | |
Modified residuei | 538 | Phosphothreonine; by PDPK1By similarity | 1 | |
Modified residuei | 676 | Phosphoserine; by autocatalysisSequence analysisBy similarity | 1 | |
Modified residuei | 685 | PhosphoserineBy similarity | 1 | |
Modified residuei | 695 | Phosphoserine; by autocatalysisSequence analysisBy similarity | 1 |
Post-translational modificationi
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q02111 |
jPOSTi | Q02111 |
MaxQBi | Q02111 |
PaxDbi | Q02111 |
PRIDEi | Q02111 |
PTM databases
iPTMneti | Q02111 |
PhosphoSitePlusi | Q02111 |
Expressioni
Tissue specificityi
T-lymphocytes and skeletal muscle.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000026778, Expressed in habenula and 197 other tissues |
ExpressionAtlasi | Q02111, baseline and differential |
Genevisiblei | Q02111, MM |
Interactioni
Subunit structurei
Part of a membrane raft complex composed at least of BCL10, CARD11, MALT1 and IKBKB (By similarity).
Interacts with GLRX3 (via N-terminus) (By similarity).
Interacts with ECT2 (By similarity).
Interacts with CCDC88A/GIV; the interaction leads to phosphorylation of CCDC88A and inhibition of its guanine nucleotide exchange factor activity (By similarity).
By similarityBinary interactionsi
Hide detailsQ02111
With | #Exp. | IntAct |
---|---|---|
Map4k3 [Q99JP0] | 2 | EBI-2639157,EBI-5324222 |
Nfix - isoform NFIX2 [P70257-2] | 3 | EBI-2639157,EBI-2639084 |
MAP4K3 [Q8IVH8] from Homo sapiens. | 4 | EBI-2639157,EBI-1758170 |
Protein-protein interaction databases
BioGRIDi | 202202, 5 interactors |
DIPi | DIP-55947N |
IntActi | Q02111, 6 interactors |
STRINGi | 10090.ENSMUSP00000028118 |
Chemistry databases
BindingDBi | Q02111 |
Miscellaneous databases
RNActi | Q02111, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q02111 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 107 | C2PROSITE-ProRule annotationAdd BLAST | 107 | |
Domaini | 380 – 634 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 635 – 706 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 72 |
Domaini
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 159 – 209 | Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 231 – 281 | Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000157638 |
HOGENOMi | CLU_000288_54_4_1 |
InParanoidi | Q02111 |
OrthoDBi | 222529at2759 |
PhylomeDBi | Q02111 |
TreeFami | TF102004 |
Family and domain databases
CDDi | cd00029, C1, 2 hits cd05619, STKc_nPKC_theta, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR035892, C2_domain_sf IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR034668, nPKC_theta IPR002219, PE/DAG-bd IPR027264, PKC_theta IPR017892, Pkinase_C IPR014376, Prot_kin_PKC_delta IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00130, C1_1, 2 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
PIRSFi | PIRSF000551, PKC_delta, 1 hit PIRSF501105, Protein_kin_C_theta, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50004, C2, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00479, ZF_DAG_PE_1, 2 hits PS50081, ZF_DAG_PE_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q02111-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPFLRIGLS NFDCGTCQAC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP
60 70 80 90 100
TMYPPWDSTF DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN
110 120 130 140 150
GRTEIWLELK PQGRMLMNAR YFLEMSDTKD MSEFENEGFF ALHQRRGAIK
160 170 180 190 200
QAKVHHVKCH EFTATFFPQP TFCSVCHEFV WGLNKQGYQC RQCNAAIHKK
210 220 230 240 250
CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK SPTFCEHCGT
260 270 280 290 300
LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ
310 320 330 340 350
ARSLRDSEHI FREGPVEIGL PCSTKNETRP PCVPTPGKRE PQGISWDSPL
360 370 380 390 400
DGSNKSAGPP EPEVSMRRTS LQLKLKIDDF ILHKMLGKGS FGKVFLAEFK
410 420 430 440 450
RTNQFFAIKA LKKDVVLMDD DVECTMVEKR VLSLAWEHPF LTHMFCTFQT
460 470 480 490 500
KENLFFVMEY LNGGDLMYHI QSCHKFDLSR ATFYAAEVIL GLQFLHSKGI
510 520 530 540 550
VYRDLKLDNI LLDRDGHIKI ADFGMCKENM LGDAKTNTFC GTPDYIAPEI
560 570 580 590 600
LLGQKYNHSV DWWSFGVLVY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR
610 620 630 640 650
WLEREAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP
660 670 680 690 700
FRPKVKSPYD CSNFDKEFLS EKPRLSFADR ALINSMDQNM FSNFSFINPG
METLICS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8WGT6 | F8WGT6_MOUSE | Protein kinase C | Prkcq | 643 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11091 mRNA Translation: BAA01864.1 |
CCDSi | CCDS15682.1 |
PIRi | A44500 |
RefSeqi | NP_032885.1, NM_008859.2 |
Genome annotation databases
Ensembli | ENSMUST00000028118; ENSMUSP00000028118; ENSMUSG00000026778 |
GeneIDi | 18761 |
KEGGi | mmu:18761 |
UCSCi | uc008iic.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11091 mRNA Translation: BAA01864.1 |
CCDSi | CCDS15682.1 |
PIRi | A44500 |
RefSeqi | NP_032885.1, NM_008859.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FKD | X-ray | 1.63 | A | 232-281 | [»] | |
SMRi | Q02111 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202202, 5 interactors |
DIPi | DIP-55947N |
IntActi | Q02111, 6 interactors |
STRINGi | 10090.ENSMUSP00000028118 |
Chemistry databases
BindingDBi | Q02111 |
ChEMBLi | CHEMBL1075295 |
PTM databases
iPTMneti | Q02111 |
PhosphoSitePlusi | Q02111 |
Proteomic databases
EPDi | Q02111 |
jPOSTi | Q02111 |
MaxQBi | Q02111 |
PaxDbi | Q02111 |
PRIDEi | Q02111 |
Protocols and materials databases
Antibodypediai | 10940, 802 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028118; ENSMUSP00000028118; ENSMUSG00000026778 |
GeneIDi | 18761 |
KEGGi | mmu:18761 |
UCSCi | uc008iic.1, mouse |
Organism-specific databases
CTDi | 5588 |
MGIi | MGI:97601, Prkcq |
Phylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000157638 |
HOGENOMi | CLU_000288_54_4_1 |
InParanoidi | Q02111 |
OrthoDBi | 222529at2759 |
PhylomeDBi | Q02111 |
TreeFami | TF102004 |
Enzyme and pathway databases
Reactomei | R-MMU-111465, Apoptotic cleavage of cellular proteins R-MMU-114508, Effects of PIP2 hydrolysis R-MMU-202424, Downstream TCR signaling R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-373752, Netrin-1 signaling R-MMU-9648002, RAS processing |
Miscellaneous databases
BioGRID-ORCSi | 18761, 3 hits in 19 CRISPR screens |
PROi | PR:Q02111 |
RNActi | Q02111, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026778, Expressed in habenula and 197 other tissues |
ExpressionAtlasi | Q02111, baseline and differential |
Genevisiblei | Q02111, MM |
Family and domain databases
CDDi | cd00029, C1, 2 hits cd05619, STKc_nPKC_theta, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR035892, C2_domain_sf IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR034668, nPKC_theta IPR002219, PE/DAG-bd IPR027264, PKC_theta IPR017892, Pkinase_C IPR014376, Prot_kin_PKC_delta IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00130, C1_1, 2 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
PIRSFi | PIRSF000551, PKC_delta, 1 hit PIRSF501105, Protein_kin_C_theta, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50004, C2, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00479, ZF_DAG_PE_1, 2 hits PS50081, ZF_DAG_PE_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KPCT_MOUSE | |
Accessioni | Q02111Primary (citable) accession number: Q02111 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index