UniProtKB - Q02085 (SNAI1_MOUSE)
Zinc finger protein SNAI1
Snai1
Functioni
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro) (By similarity).
During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (PubMed:24239292).
SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (PubMed:24239292).
Associates with EGR1 and SP1 to mediate 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3'. In addition, may also activate the CDKN2B promoter by itself.
By similarity3 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 156 – 176 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 180 – 202 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 210 – 230 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 236 – 259 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- E-box binding Source: MGI
- kinase binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cartilage morphogenesis Source: MGI
- cell migration Source: MGI
- epithelial to mesenchymal transition Source: UniProtKB
- epithelial to mesenchymal transition involved in endocardial cushion formation Source: BHF-UCL
- hair follicle morphogenesis Source: MGI
- heterochromatin organization Source: UniProtKB
- left/right pattern formation Source: MGI
- mesoderm development Source: MGI
- mesoderm formation Source: UniProtKB
- negative regulation of cell differentiation involved in embryonic placenta development Source: MGI
- negative regulation of DNA damage response, signal transduction by p53 class mediator Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- negative regulation of vitamin D biosynthetic process Source: MGI
- Notch signaling involved in heart development Source: BHF-UCL
- osteoblast differentiation Source: Ensembl
- positive regulation of cell migration Source: BHF-UCL
- positive regulation of epithelial to mesenchymal transition Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of bicellular tight junction assembly Source: MGI
- regulation of transcription, DNA-templated Source: GO_Central
- regulation of transcription by RNA polymerase II Source: MGI
- roof of mouth development Source: MGI
- trophoblast giant cell differentiation Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein SNAI1Alternative name(s): Protein snail homolog 1 Short name: Protein sna |
Gene namesi | Name:Snai1 Synonyms:Sna |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98330, Snai1 |
VEuPathDBi | HostDB:ENSMUSG00000042821 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- fibrillar center Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: MGI
- pericentric heterochromatin Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047030 | 1 – 264 | Zinc finger protein SNAI1Add BLAST | 264 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 82 | PhosphoserineBy similarity | 1 | |
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 96 | PhosphoserineBy similarity | 1 | |
Cross-linki | 98 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 100 | PhosphoserineBy similarity | 1 | |
Modified residuei | 104 | PhosphoserineBy similarity | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 111 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 112 | O-linked (GlcNAc) serineBy similarity | 1 | |
Modified residuei | 115 | PhosphoserineBy similarity | 1 | |
Modified residuei | 119 | PhosphoserineBy similarity | 1 | |
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 146 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 203 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 246 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q02085 |
PaxDbi | Q02085 |
PRIDEi | Q02085 |
ProteomicsDBi | 261285 |
PTM databases
GlyGeni | Q02085, 1 site |
iPTMneti | Q02085 |
PhosphoSitePlusi | Q02085 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000042821, Expressed in ectoplacental cone and 239 other tissues |
ExpressionAtlasi | Q02085, baseline and differential |
Genevisiblei | Q02085, MM |
Interactioni
Subunit structurei
Interacts with LOXL2 and LOXL3 (By similarity).
Interacts with FBXL14 and GSK3B.
Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif.
Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).
Interacts (via N-terminal region) with CSNK2A1.
Interacts with EGR1 upon TPA induction.
Interacts (via N-terminal region) with LATS2; the interaction is dependent on LATS2 kinase activity but independent of SNAI1 Thr-203 phosphorylation.
Interacts (via zinc fingers) with KPNB1 and TNPO1; the interactions mediate nuclear import.
Interacts (via zinc fingers) with KPNA1; the interaction disrupts the transport complex with KPNB1 and prevents nuclear import increasing SNAI1 degradation in the cytoplasm.
Interacts (via zinc fingers) with KPNA2; the interaction, in combination with KPNB1, mediates nuclear import.
Interacts with KPNA4; this interaction mediates nuclear import. May interact (via zinc fingers) with IPO7.
Interacts (via zinc fingers) with PARP1; the interaction requires SNAI1 to be poly-ADP-ribosylated and non-phosphorylated (active) by GSK3B.
Interacts (via SNAG domain) with KDM1A; the interaction is necessary for the down-regulation of dimethylated H3K4 mark and promoter activity of E-cadherin/CDH1, CDN7 and KRT8.
Interacts with TP53/p53 and (via zinc fingers) with NOTCH1 (via intracellular domain); the interactions induce SNAI1 degradation via MDM2-mediated ubiquitination and inhibit SNAI1-induced cell invasion.
Interacts with MDM2; the interaction promotes SNAI1 ubiquitination.
Interacts (via zinc fingers) with CSNK1E.
Interacts with PAK1.
By similarity4 PublicationsBinary interactionsi
Q02085
With | #Exp. | IntAct |
---|---|---|
PARP1 [P09874] from Homo sapiens. | 3 | EBI-6049807,EBI-355676 |
GO - Molecular functioni
- kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203361, 32 interactors |
IntActi | Q02085, 2 interactors |
STRINGi | 10090.ENSMUSP00000050581 |
Miscellaneous databases
RNActi | Q02085, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | SNAG domainCuratedAdd BLAST | 20 | |
Regioni | 2 – 7 | Required and sufficient for interaction with KDM1ABy similarity | 6 | |
Regioni | 10 – 40 | LATS2 bindingBy similarityAdd BLAST | 31 | |
Regioni | 85 – 116 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 120 – 151 | Required for FBXL14-triggered degradationBy similarityAdd BLAST | 32 | |
Regioni | 151 – 264 | Required for nuclear localization and interaction with KPNB1, NOTCH1 and PARP1By similarityAdd BLAST | 114 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 95 – 100 | Destruction motif | 6 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 156 – 176 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 180 – 202 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 210 – 230 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 236 – 259 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154681 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | Q02085 |
OMAi | GWASFRP |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q02085 |
TreeFami | TF315515 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPRSFLVRKP SDPRRKPNYS ELQDACVEFT FQQPYDQAHL LAAIPPPEVL
60 70 80 90 100
NPAASLPTLI WDSLLVPQVR PVAWATLPLR ESPKAVELTS LSDEDSGKSS
110 120 130 140 150
QPPSPPSPAP SSFSSTSASS LEAEAFIAFP GLGQLPKQLA RLSVAKDPQS
160 170 180 190 200
RKIFNCKYCN KEYLSLGALK MHIRSHTLPC VCTTCGKAFS RPWLLQGHVR
210 220 230 240 250
THTGEKPFSC SHCNRAFADR SNLRAHLQTH SDVKRYQCQA CARTFSRMSL
260
LHKHQESGCS GGPR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 219 | D → V in CAA47675 (PubMed:1483390).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95604 mRNA Translation: AAA03481.1 X67253 mRNA Translation: CAA47675.1 BC034857 mRNA Translation: AAH34857.1 U95961 Genomic DNA Translation: AAB58054.1 |
CCDSi | CCDS17102.1 |
PIRi | A49149 |
RefSeqi | NP_035557.1, NM_011427.3 |
Genome annotation databases
Ensembli | ENSMUST00000052631; ENSMUSP00000050581; ENSMUSG00000042821 |
GeneIDi | 20613 |
KEGGi | mmu:20613 |
UCSCi | uc008nzy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95604 mRNA Translation: AAA03481.1 X67253 mRNA Translation: CAA47675.1 BC034857 mRNA Translation: AAH34857.1 U95961 Genomic DNA Translation: AAB58054.1 |
CCDSi | CCDS17102.1 |
PIRi | A49149 |
RefSeqi | NP_035557.1, NM_011427.3 |
3D structure databases
AlphaFoldDBi | Q02085 |
SMRi | Q02085 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203361, 32 interactors |
IntActi | Q02085, 2 interactors |
STRINGi | 10090.ENSMUSP00000050581 |
PTM databases
GlyGeni | Q02085, 1 site |
iPTMneti | Q02085 |
PhosphoSitePlusi | Q02085 |
Proteomic databases
jPOSTi | Q02085 |
PaxDbi | Q02085 |
PRIDEi | Q02085 |
ProteomicsDBi | 261285 |
Protocols and materials databases
Antibodypediai | 3135, 900 antibodies from 46 providers |
DNASUi | 20613 |
Genome annotation databases
Ensembli | ENSMUST00000052631; ENSMUSP00000050581; ENSMUSG00000042821 |
GeneIDi | 20613 |
KEGGi | mmu:20613 |
UCSCi | uc008nzy.1, mouse |
Organism-specific databases
CTDi | 6615 |
MGIi | MGI:98330, Snai1 |
VEuPathDBi | HostDB:ENSMUSG00000042821 |
Phylogenomic databases
eggNOGi | KOG2462, Eukaryota |
GeneTreei | ENSGT00940000154681 |
HOGENOMi | CLU_002678_42_3_1 |
InParanoidi | Q02085 |
OMAi | GWASFRP |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q02085 |
TreeFami | TF315515 |
Miscellaneous databases
BioGRID-ORCSi | 20613, 10 hits in 78 CRISPR screens |
ChiTaRSi | Snai1, mouse |
PROi | PR:Q02085 |
RNActi | Q02085, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042821, Expressed in ectoplacental cone and 239 other tissues |
ExpressionAtlasi | Q02085, baseline and differential |
Genevisiblei | Q02085, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SNAI1_MOUSE | |
Accessioni | Q02085Primary (citable) accession number: Q02085 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families