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Protein

Achaete-scute homolog 1

Gene

Ascl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways (PubMed:24243019). Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes (PubMed:20107439, PubMed:24243019, PubMed:27281220). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS (PubMed:8217843). Essential for the generation of olfactory and autonomic neurons (PubMed:8221886). Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation (PubMed:16020526, PubMed:17728344).7 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Achaete-scute homolog 12 Publications
Short name:
ASH-12 Publications
Short name:
mASH-12 Publications
Short name:
mASH12 Publications
Gene namesi
Name:Ascl1Imported
Synonyms:Ash12 Publications, Mash-12 Publications, Mash12 Publications
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96919 Ascl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethality at birth caused by severe defects in neurogenesis. While the brain and spinal cord of the mutants appear normal, their olfactory epithelium and sympathetic, parasympathetic and enteric ganglia are severely affected. In the olfactory epithelium, neuronal progenitors die at an early stage, whereas the non-neuronal supporting cells are present. In sympathetic ganglia, the development of neuronal precursors is arrested, preventing the generation of sympathetic neurons, without affecting glial precursor cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271271 – 231Achaete-scute homolog 1Add BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ02067
PRIDEiQ02067

PTM databases

iPTMnetiQ02067
PhosphoSitePlusiQ02067

Expressioni

Tissue specificityi

Developing CNS and PNS at embryonic and post-natal stages.3 Publications

Developmental stagei

Between E8.5 and E10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between E10.5 and E12.5 its expression pattern changes from a restricted to a widespread zone, it is then found at variable levels in the ventricular zone in all regions of the brain, where is expressed in a subset of p2 progenitors that can give rise to either V2a or V2b interneuron subtypes. From E12.5 to postnatal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina.3 Publications

Gene expression databases

BgeeiENSMUSG00000020052 Expressed in 156 organ(s), highest expression level in olfactory epithelium
CleanExiMM_ASCL1
GenevisibleiQ02067 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201314, 5 interactors
STRINGi10090.ENSMUSP00000020243

Structurei

3D structure databases

ProteinModelPortaliQ02067
SMRiQ02067
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 165bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 43Poly-AlaAdd BLAST14
Compositional biasi47 – 52Poly-Gln6

Phylogenomic databases

eggNOGiKOG4029 Eukaryota
ENOG411227D LUCA
GeneTreeiENSGT00530000063321
HOGENOMiHOG000013056
HOVERGENiHBG050590
InParanoidiQ02067
KOiK09067
OMAiELMRCKR
OrthoDBiEOG091G0OB6
TreeFamiTF322889

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR015660 MASH1/Ascl1a-like
PANTHERiPTHR13935 PTHR13935, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q02067-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAQSAQQQQ
60 70 80 90 100
PQAPPQQAPQ LSPVADSQPS GGGHKSAAKQ VKRQRSSSPE LMRCKRRLNF
110 120 130 140 150
SGFGYSLPQQ QPAAVARRNE RERNRVKLVN LGFATLREHV PNGAANKKMS
160 170 180 190 200
KVETLRSAVE YIRALQQLLD EHDAVSAAFQ AGVLSPTISP NYSNDLNSMA
210 220 230
GSPVSSYSSD EGSYDPLSPE EQELLDFTNW F
Length:231
Mass (Da):24,741
Last modified:October 3, 2012 - v2
Checksum:i454E6937520BD4CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81V → D in AAA37780 (PubMed:8424959).Curated1
Sequence conflicti160E → Q in AAA37780 (PubMed:8424959).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95603 mRNA Translation: AAA37780.1
AK143210 mRNA Translation: BAE25307.1
AC122360 Genomic DNA No translation available.
CH466539 Genomic DNA Translation: EDL21455.1
BC055748 mRNA Translation: AAH55748.1
CCDSiCCDS24101.1
PIRiS28186
RefSeqiNP_032579.2, NM_008553.4
UniGeneiMm.136217

Genome annotation databases

EnsembliENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052
GeneIDi17172
KEGGimmu:17172
UCSCiuc007gqs.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95603 mRNA Translation: AAA37780.1
AK143210 mRNA Translation: BAE25307.1
AC122360 Genomic DNA No translation available.
CH466539 Genomic DNA Translation: EDL21455.1
BC055748 mRNA Translation: AAH55748.1
CCDSiCCDS24101.1
PIRiS28186
RefSeqiNP_032579.2, NM_008553.4
UniGeneiMm.136217

3D structure databases

ProteinModelPortaliQ02067
SMRiQ02067
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201314, 5 interactors
STRINGi10090.ENSMUSP00000020243

PTM databases

iPTMnetiQ02067
PhosphoSitePlusiQ02067

Proteomic databases

PaxDbiQ02067
PRIDEiQ02067

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020243; ENSMUSP00000020243; ENSMUSG00000020052
GeneIDi17172
KEGGimmu:17172
UCSCiuc007gqs.1 mouse

Organism-specific databases

CTDi429
MGIiMGI:96919 Ascl1

Phylogenomic databases

eggNOGiKOG4029 Eukaryota
ENOG411227D LUCA
GeneTreeiENSGT00530000063321
HOGENOMiHOG000013056
HOVERGENiHBG050590
InParanoidiQ02067
KOiK09067
OMAiELMRCKR
OrthoDBiEOG091G0OB6
TreeFamiTF322889

Miscellaneous databases

PROiPR:Q02067
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020052 Expressed in 156 organ(s), highest expression level in olfactory epithelium
CleanExiMM_ASCL1
GenevisibleiQ02067 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR015660 MASH1/Ascl1a-like
PANTHERiPTHR13935 PTHR13935, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASCL1_MOUSE
AccessioniPrimary (citable) accession number: Q02067
Secondary accession number(s): Q7TNT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 3, 2012
Last modified: September 12, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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