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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 25
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi432 – 439ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 25
Taxonomic identifieri10609 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeFirstpapillomavirinaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009162 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331231 – 604Replication protein E1Add BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Phosphoserine; by hostUniRule annotation1
Modified residuei89Phosphoserine; by hostUniRule annotation1
Cross-linki513Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylated.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ02049
SMRiQ02049
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini406 – 556SF3 helicaseUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 307DNA-binding regionUniRule annotationAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 78Nuclear localization signalUniRule annotation3

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000HF

Family and domain databases

HAMAPiMF_04000 PPV_E1, 1 hit
InterProiView protein in InterPro
IPR001177 DNA_helicase_E1_C_Papillomavir
IPR014000 DNA_helicase_E1_N_Papillomavir
IPR014015 Helicase_SF3_DNA-vir
IPR027417 P-loop_NTPase
IPR016393 Rep_E1_papillomaV
PfamiView protein in Pfam
PF00519 PPV_E1_C, 1 hit
PF00524 PPV_E1_N, 1 hit
PIRSFiPIRSF003383 Rep_E1_papillomaV, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51206 SF3_HELICASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q02049-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPKGSTSK EGFNDWCILE AECSDIDNDL EQLFDQDTDS DISDLLDEND
60 70 80 90 100
VEQGNSRELF HLQECQESEE QLQKLKRKYL SPKAVAQLSP RFESISLSPQ
110 120 130 140 150
QKSKRRLFAE QDSGLELTLT NEAEDVSPEV EVPALNSQPV AEGQSGDIDI
160 170 180 190 200
SYTALLRASN NKAILMAKFK EAFGVGFNDL TRQFKSYKTC CNAWVISVYA
210 220 230 240 250
VHDDLIESSK QLLQQHCDYV WIRGIGAMSL FLVCFKAGKN RGTVHKLMTT
260 270 280 290 300
MLNVHEKQIL SEPPKLRNVA AALFWYKGSM GSGVFTYGSY PDWIAHQTIL
310 320 330 340 350
GHQSAEASTF DLSDMVQWAF DNNYLDEADI AYQYAKLAPD NSNAVAWLAH
360 370 380 390 400
NNQAKFVREC ASMVRFYKKG QMKEMSMSEW IYTKIHEVEG EGQWSTIVQF
410 420 430 440 450
LRYQQVNFIM FLAALKDLLH SVPKRNCILF YGPPNTGKSA FTMSLIKVLK
460 470 480 490 500
GRVLSFCNSK SQFWLQPLSE CKIALLDDVT DPCWVYMDTY LRNGLDGHYV
510 520 530 540 550
SLDCKHKAPM QTKFPALLLT SNINVHNEVN YRYLHSRIKG FEFPNPFPMK
560 570 580 590 600
ADNTPQFDLT DQSWKSFFTR LWHQLDLSDQ EDEGENGESQ RAFQCSTRSA

NEHL
Length:604
Mass (Da):68,926
Last modified:June 1, 1994 - v2
Checksum:i0A98EB84E93B19B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74471 Genomic DNA Translation: CAA52526.1
M96321 Genomic DNA Translation: AAA47010.1
PIRiS36493

Similar proteinsi

Entry informationi

Entry nameiVE1_HPV25
AccessioniPrimary (citable) accession number: Q02049
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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