UniProtKB - Q02013 (AQP1_MOUSE)
Protein
Aquaporin-1
Gene
Aqp1
Organism
Mus musculus (Mouse)
Status
Functioni
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.1 Publication
Miscellaneous
Pharmacologically inhibited by submillimolar concentrations of mercury.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 56 | Substrate discriminationBy similarity | 1 | |
Sitei | 180 | Substrate discriminationBy similarity | 1 | |
Sitei | 189 | Hg(2+)-sensitive residueBy similarity | 1 | |
Sitei | 195 | Substrate discriminationBy similarity | 1 |
GO - Molecular functioni
- ammonium transmembrane transporter activity Source: UniProtKB
- carbon dioxide transmembrane transporter activity Source: UniProtKB
- ephrin receptor binding Source: UniProtKB
- glycerol transmembrane transporter activity Source: UniProtKB
- identical protein binding Source: MGI
- intracellular cGMP-activated cation channel activity Source: UniProtKB
- nitric oxide transmembrane transporter activity Source: UniProtKB
- potassium channel activity Source: UniProtKB
- potassium ion transmembrane transporter activity Source: UniProtKB
- transmembrane transporter activity Source: MGI
- water channel activity Source: UniProtKB
- water transmembrane transporter activity Source: MGI
GO - Biological processi
- ammonium transport Source: UniProtKB
- camera-type eye morphogenesis Source: MGI
- carbon dioxide transmembrane transport Source: UniProtKB
- carbon dioxide transport Source: UniProtKB
- cellular homeostasis Source: UniProtKB
- cellular hyperosmotic response Source: UniProtKB
- cellular response to cAMP Source: UniProtKB
- cellular response to copper ion Source: UniProtKB
- cellular response to dexamethasone stimulus Source: UniProtKB
- cellular response to hydrogen peroxide Source: UniProtKB
- cellular response to hypoxia Source: UniProtKB
- cellular response to inorganic substance Source: UniProtKB
- cellular response to mechanical stimulus Source: UniProtKB
- cellular response to mercury ion Source: UniProtKB
- cellular response to nitric oxide Source: MGI
- cellular response to retinoic acid Source: UniProtKB
- cellular response to salt stress Source: UniProtKB
- cellular response to UV Source: UniProtKB
- cellular water homeostasis Source: UniProtKB
- cell volume homeostasis Source: UniProtKB
- cerebrospinal fluid secretion Source: Ensembl
- cGMP-mediated signaling Source: UniProtKB
- corticotropin secretion Source: MGI
- defense response to Gram-negative bacterium Source: UniProtKB
- establishment or maintenance of actin cytoskeleton polarity Source: UniProtKB
- glomerular filtration Source: MGI
- glycerol transport Source: UniProtKB
- hyperosmotic response Source: GO_Central
- hyperosmotic salinity response Source: Ensembl
- lateral ventricle development Source: UniProtKB
- lipid digestion Source: MGI
- maintenance of symbiont-containing vacuole by host Source: UniProtKB
- metanephric descending thin limb development Source: UniProtKB
- metanephric glomerulus vasculature development Source: UniProtKB
- metanephric proximal convoluted tubule segment 2 development Source: UniProtKB
- metanephric proximal straight tubule development Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- nitric oxide transport Source: UniProtKB
- odontogenesis Source: Ensembl
- pancreatic juice secretion Source: Ensembl
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of cell migration Source: MGI
- positive regulation of epithelial cell migration Source: MGI
- positive regulation of fibroblast proliferation Source: UniProtKB
- positive regulation of lamellipodium assembly Source: MGI
- positive regulation of saliva secretion Source: UniProtKB
- potassium ion transport Source: UniProtKB
- renal water absorption Source: MGI
- renal water transport Source: MGI
- response to estrogen Source: Ensembl
- secretion by cell Source: MGI
- secretory granule organization Source: MGI
- sensory perception of pain Source: MGI
- transepithelial water transport Source: UniProtKB
- water homeostasis Source: MGI
- water transport Source: MGI
- wound healing Source: MGI
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-MMU-432047, Passive transport by Aquaporins |
Names & Taxonomyi
Protein namesi | Recommended name: Aquaporin-1Short name: AQP-1 Alternative name(s): Aquaporin-CHIP Delayed early response protein 2 Short name: DER2 Water channel protein for red blood cells and kidney proximal tubule |
Gene namesi | Name:Aqp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103201, Aqp1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Extracellular region or secreted
- extracellular exosome Source: MGI
- symbiont-containing vacuole Source: UniProtKB
Nucleus
- nuclear membrane Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- basal plasma membrane Source: BHF-UCL
- basolateral plasma membrane Source: UniProtKB
- brush border membrane Source: UniProtKB
- caveola Source: BHF-UCL
- integral component of plasma membrane Source: MGI
- neuronal cell body membrane Source: MGI
- plasma membrane Source: MGI
- sarcolemma Source: UniProtKB
Other locations
- apical part of cell Source: MGI
- axon Source: MGI
- axon terminus Source: MGI
- brush border Source: UniProtKB
- cytoplasm Source: UniProtKB
- dense core granule membrane Source: MGI
- integral component of membrane Source: MGI
- membrane Source: MGI
- neuron projection Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicBy similarity | 7 | |
Transmembranei | 8 – 36 | Helical; Name=Helix 1By similarityAdd BLAST | 29 | |
Topological domaini | 37 – 48 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 49 – 66 | Helical; Name=Helix 2By similarityAdd BLAST | 18 | |
Topological domaini | 67 – 70 | CytoplasmicBy similarity | 4 | |
Intramembranei | 71 – 76 | By similarity | 6 | |
Intramembranei | 77 – 84 | Helical; Name=Helix BBy similarity | 8 | |
Topological domaini | 85 – 94 | CytoplasmicBy similarity | 10 | |
Transmembranei | 95 – 115 | Helical; Name=Helix 3By similarityAdd BLAST | 21 | |
Topological domaini | 116 – 136 | ExtracellularBy similarityAdd BLAST | 21 | |
Transmembranei | 137 – 155 | Helical; Name=Helix 4By similarityAdd BLAST | 19 | |
Topological domaini | 156 – 166 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 167 – 183 | Helical; Name=Helix 5By similarityAdd BLAST | 17 | |
Topological domaini | 184 – 186 | ExtracellularBy similarity | 3 | |
Intramembranei | 187 – 192 | By similarity | 6 | |
Intramembranei | 193 – 200 | Helical; Name=Helix EBy similarity | 8 | |
Topological domaini | 201 – 207 | ExtracellularBy similarity | 7 | |
Transmembranei | 208 – 228 | Helical; Name=Helix 6By similarityAdd BLAST | 21 | |
Topological domaini | 229 – 269 | CytoplasmicBy similarityAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutant mice are born at the expected Mendelian rate and appear healthy and normal, but display strongly reduced urine osmolality. Besides, their erythrocytes show reduced water permeability. Mice lacking both Aqp1 and Slc14a1 are born at the expected Mendelian ratio, but do not thrive; half of them die within ten days after birth and none are alive after two weeks. Urine osmolality is somewhat lower than that observed with mice lacking only Aqp1. Besides, erythrocyte water permeability is significantly lower than in mice lacking only Aqp1.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063921 | 1 – 269 | Aquaporin-1Add BLAST | 269 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 205 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 247 | PhosphoserineCombined sources | 1 | |
Modified residuei | 253 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 262 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q02013 |
MaxQBi | Q02013 |
PaxDbi | Q02013 |
PeptideAtlasi | Q02013 |
PRIDEi | Q02013 |
PTM databases
GlyGeni | Q02013, 1 site |
iPTMneti | Q02013 |
PhosphoSitePlusi | Q02013 |
SwissPalmi | Q02013 |
Expressioni
Tissue specificityi
Detected in erythrocytes (at protein level) (PubMed:12133842). In the kidney, expressed on luminal and basal borders of proximal tubules and in the thin limb of Henle's loop (at protein level) (PubMed:31605441).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000004655, Expressed in decidua and 259 other tissues |
Genevisiblei | Q02013, MM |
Interactioni
Subunit structurei
Homotetramer.
Identified in a complex with STOM (By similarity).
Interacts with EPHB2; involved in endolymph production in the inner ear.
By similarity1 PublicationGO - Molecular functioni
- ephrin receptor binding Source: UniProtKB
- identical protein binding Source: MGI
Protein-protein interaction databases
IntActi | Q02013, 3 interactors |
MINTi | Q02013 |
STRINGi | 10090.ENSMUSP00000004774 |
Miscellaneous databases
RNActi | Q02013, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 76 – 78 | NPA 1 | 3 | |
Motifi | 192 – 194 | NPA 2 | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 159 – 162 | Poly-Arg | 4 |
Domaini
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000157015 |
HOGENOMi | CLU_020019_3_3_1 |
InParanoidi | Q02013 |
OMAi | ELFGTFW |
OrthoDBi | 1152704at2759 |
PhylomeDBi | Q02013 |
TreeFami | TF312940 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023274, Aquaporin_1 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02013, AQUAPORIN1 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q02013-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVATA ILSGITSSLV DNSLGRNDLA HGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGG ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 190 | G → S in AAH07125 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02914 mRNA Translation: AAB53928.1 AK081886 mRNA Translation: BAC38360.1 AK086688 mRNA Translation: BAC39719.1 AK157333 mRNA Translation: BAE34051.1 AK158226 mRNA Translation: BAE34412.1 AK158389 mRNA Translation: BAE34482.1 AK171627 mRNA Translation: BAE42573.1 AK172361 mRNA Translation: BAE42966.1 BC007125 mRNA Translation: AAH07125.1 |
CCDSi | CCDS20164.1 |
PIRi | B44499 |
RefSeqi | NP_031498.1, NM_007472.2 |
Genome annotation databases
Ensembli | ENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655 |
GeneIDi | 11826 |
KEGGi | mmu:11826 |
UCSCi | uc009caq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02914 mRNA Translation: AAB53928.1 AK081886 mRNA Translation: BAC38360.1 AK086688 mRNA Translation: BAC39719.1 AK157333 mRNA Translation: BAE34051.1 AK158226 mRNA Translation: BAE34412.1 AK158389 mRNA Translation: BAE34482.1 AK171627 mRNA Translation: BAE42573.1 AK172361 mRNA Translation: BAE42966.1 BC007125 mRNA Translation: AAH07125.1 |
CCDSi | CCDS20164.1 |
PIRi | B44499 |
RefSeqi | NP_031498.1, NM_007472.2 |
3D structure databases
SMRi | Q02013 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q02013, 3 interactors |
MINTi | Q02013 |
STRINGi | 10090.ENSMUSP00000004774 |
PTM databases
GlyGeni | Q02013, 1 site |
iPTMneti | Q02013 |
PhosphoSitePlusi | Q02013 |
SwissPalmi | Q02013 |
Proteomic databases
jPOSTi | Q02013 |
MaxQBi | Q02013 |
PaxDbi | Q02013 |
PeptideAtlasi | Q02013 |
PRIDEi | Q02013 |
Genome annotation databases
Ensembli | ENSMUST00000004774; ENSMUSP00000004774; ENSMUSG00000004655 |
GeneIDi | 11826 |
KEGGi | mmu:11826 |
UCSCi | uc009caq.1, mouse |
Organism-specific databases
CTDi | 358 |
MGIi | MGI:103201, Aqp1 |
Phylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000157015 |
HOGENOMi | CLU_020019_3_3_1 |
InParanoidi | Q02013 |
OMAi | ELFGTFW |
OrthoDBi | 1152704at2759 |
PhylomeDBi | Q02013 |
TreeFami | TF312940 |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-MMU-432047, Passive transport by Aquaporins |
Miscellaneous databases
BioGRID-ORCSi | 11826, 0 hits in 18 CRISPR screens |
ChiTaRSi | Aqp1, mouse |
PROi | PR:Q02013 |
RNActi | Q02013, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004655, Expressed in decidua and 259 other tissues |
Genevisiblei | Q02013, MM |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR023274, Aquaporin_1 IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR02013, AQUAPORIN1 PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AQP1_MOUSE | |
Accessioni | Q02013Primary (citable) accession number: Q02013 Secondary accession number(s): Q542P1, Q91VY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families