Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

PLCB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei379PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.4.11 2681
ReactomeiR-HSA-112043 PLC beta mediated events
R-HSA-1855204 Synthesis of IP3 and IP4 in the cytosol
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-4086398 Ca2+ pathway
R-HSA-416476 G alpha (q) signalling events
R-HSA-418217 G beta:gamma signalling through PLC beta
R-HSA-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-HSA-500657 Presynaptic function of Kainate receptors
SIGNORiQ01970

Chemistry databases

SwissLipidsiSLP:000001756

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name:
PLC-beta-3
Gene namesi
Name:PLCB3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149782.11
HGNCiHGNC:9056 PLCB3
MIMi600230 gene
neXtProtiNX_Q01970

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5331
OpenTargetsiENSG00000149782
PharmGKBiPA33386

Chemistry databases

ChEMBLiCHEMBL5449

Polymorphism and mutation databases

BioMutaiPLCB3
DMDMi1730573

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000884912 – 12341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3Add BLAST1233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei474PhosphoserineCombined sources1
Modified residuei490PhosphoserineCombined sources1
Modified residuei495PhosphoserineCombined sources1
Modified residuei537PhosphoserineCombined sources1
Modified residuei926PhosphoserineCombined sources1
Modified residuei1105PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ01970
MaxQBiQ01970
PaxDbiQ01970
PeptideAtlasiQ01970
PRIDEiQ01970
ProteomicsDBi58024

PTM databases

iPTMnetiQ01970
PhosphoSitePlusiQ01970

Expressioni

Gene expression databases

BgeeiENSG00000149782 Expressed in 180 organ(s), highest expression level in small intestine Peyer's patch
CleanExiHS_PLCB3
GenevisibleiQ01970 HS

Organism-specific databases

HPAiCAB009257
HPA039279
HPA043907

Interactioni

Subunit structurei

Interacts with SHANK2 (By similarity). Interacts with LPAR2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GnaqP212796EBI-4289548,EBI-771975From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111347, 19 interactors
CORUMiQ01970
DIPiDIP-41928N
IntActiQ01970, 6 interactors
MINTiQ01970
STRINGi9606.ENSP00000279230

Chemistry databases

BindingDBiQ01970

Structurei

Secondary structure

11234
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ01970
SMRiQ01970
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 468PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST151
Domaini590 – 706PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini713 – 810C2PROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1231 – 1234Interaction with SHANK2By similarity4

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiQ01970
KOiK05858
OMAiPHHHGCS
OrthoDBiEOG091G00XL
PhylomeDBiQ01970
TreeFamiTF313216

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q01970-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGAQPGVHA LQLEPPTVVE TLRRGSKFIK WDEETSSRNL VTLRVDPNGF
60 70 80 90 100
FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDARLE
110 120 130 140 150
EKLMTVVSGP DPVNTVFLNF MAVQDDTAKV WSEELFKLAM NILAQNASRN
160 170 180 190 200
TFLRKAYTKL KLQVNQDGRI PVKNILKMFS ADKKRVETAL ESCGLKFNRS
210 220 230 240 250
ESIRPDEFSL EIFERFLNKL CLRPDIDKIL LEIGAKGKPY LTLEQLMDFI
260 270 280 290 300
NQKQRDPRLN EVLYPPLRPS QARLLIEKYE PNQQFLERDQ MSMEGFSRYL
310 320 330 340 350
GGEENGILPL EALDLSTDMT QPLSAYFINS SHNTYLTAGQ LAGTSSVEMY
360 370 380 390 400
RQALLWGCRC VELDVWKGRP PEEEPFITHG FTMTTEVPLR DVLEAIAETA
410 420 430 440 450
FKTSPYPVIL SFENHVDSAK QQAKMAEYCR SIFGDALLIE PLDKYPLAPG
460 470 480 490 500
VPLPSPQDLM GRILVKNKKR HRPSAGGPDS AGRKRPLEQS NSALSESSAA
510 520 530 540 550
TEPSSPQLGS PSSDSCPGLS NGEEVGLEKP SLEPQKSLGD EGLNRGPYVL
560 570 580 590 600
GPADREDEEE DEEEEEQTDP KKPTTDEGTA SSEVNATEEM STLVNYIEPV
610 620 630 640 650
KFKSFEAARK RNKCFEMSSF VETKAMEQLT KSPMEFVEYN KQQLSRIYPK
660 670 680 690 700
GTRVDSSNYM PQLFWNVGCQ LVALNFQTLD VAMQLNAGVF EYNGRSGYLL
710 720 730 740 750
KPEFMRRPDK SFDPFTEVIV DGIVANALRV KVISGQFLSD RKVGIYVEVD
760 770 780 790 800
MFGLPVDTRR KYRTRTSQGN SFNPVWDEEP FDFPKVVLPT LASLRIAAFE
810 820 830 840 850
EGGKFVGHRI LPVSAIRSGY HYVCLRNEAN QPLCLPALLI YTEASDYIPD
860 870 880 890 900
DHQDYAEALI NPIKHVSLMD QRARQLAALI GESEAQAGQE TCQDTQSQQL
910 920 930 940 950
GSQPSSNPTP SPLDASPRRP PGPTTSPAST SLSSPGQRDD LIASILSEVA
960 970 980 990 1000
PTPLDELRGH KALVKLRSRQ ERDLRELRKK HQRKAVTLTR RLLDGLAQAQ
1010 1020 1030 1040 1050
AEGRCRLRPG ALGGAADVED TKEGEDEAKR YQEFQNRQVQ SLLELREAQV
1060 1070 1080 1090 1100
DAEAQRRLEH LRQALQRLRE VVLDANTTQF KRLKEMNERE KKELQKILDR
1110 1120 1130 1140 1150
KRHNSISEAK MRDKHKKEAE LTEINRRHIT ESVNSIRRLE EAQKQRHDRL
1160 1170 1180 1190 1200
VAGQQQVLQQ LAEEEPKLLA QLAQECQEQR ARLPQEIRRS LLGEMPEGLG
1210 1220 1230
DGPLVACASN GHAPGSSGHL SGADSESQEE NTQL
Length:1,234
Mass (Da):138,799
Last modified:October 1, 1996 - v2
Checksum:iC5106EFBA8037788
GO
Isoform 2 (identifier: Q01970-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-99: Missing.

Note: No experimental confirmation available.
Show »
Length:1,167
Mass (Da):131,205
Checksum:i597C35D7C25D4773
GO

Sequence cautioni

The sequence AAH32659 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti845S → L in CAA78903 (PubMed:1333955).Curated1
Sequence conflicti881G → E in CAA85776 (PubMed:7607669).Curated1
Sequence conflicti1089 – 1119REKKE…HKKEA → SWPSWPRSVRSSGRGSPRRS AGACWARCRRG in CAA85776 (PubMed:7607669).CuratedAdd BLAST31

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029229483R → H. Corresponds to variant dbSNP:rs12146487Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04605433 – 99Missing in isoform 2. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26425 Genomic DNA Translation: AAA77683.1
Z37544
, Z37545, Z37546, Z37547, Z37548, Z37549, Z37550, Z37551, Z37552, Z37553, Z37554, Z37555, Z37556, Z37557, Z37558, Z37559, Z37560, Z37561, Z37562, Z37564, Z37565, Z37566, Z37567, Z37568, Z37569, Z37570, Z37571, Z37572, Z37573, Z37574 Genomic DNA Translation: CAA85776.1
AP001453 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74230.1
CH471076 Genomic DNA Translation: EAW74231.1
BC032659 mRNA Translation: AAH32659.1 Different initiation.
BC142681 mRNA Translation: AAI42682.1
Z16411 mRNA Translation: CAA78903.1
CCDSiCCDS53654.1 [Q01970-2]
CCDS8064.1 [Q01970-1]
PIRiI38994
S27002
S52099
RefSeqiNP_000923.1, NM_000932.2 [Q01970-1]
NP_001171812.1, NM_001184883.1 [Q01970-2]
NP_001303243.1, NM_001316314.1 [Q01970-1]
UniGeneiHs.523761

Genome annotation databases

EnsembliENST00000279230; ENSP00000279230; ENSG00000149782 [Q01970-1]
ENST00000325234; ENSP00000324660; ENSG00000149782 [Q01970-2]
ENST00000540288; ENSP00000443631; ENSG00000149782 [Q01970-1]
GeneIDi5331
KEGGihsa:5331
UCSCiuc009ypg.3 human [Q01970-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26425 Genomic DNA Translation: AAA77683.1
Z37544
, Z37545, Z37546, Z37547, Z37548, Z37549, Z37550, Z37551, Z37552, Z37553, Z37554, Z37555, Z37556, Z37557, Z37558, Z37559, Z37560, Z37561, Z37562, Z37564, Z37565, Z37566, Z37567, Z37568, Z37569, Z37570, Z37571, Z37572, Z37573, Z37574 Genomic DNA Translation: CAA85776.1
AP001453 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74230.1
CH471076 Genomic DNA Translation: EAW74231.1
BC032659 mRNA Translation: AAH32659.1 Different initiation.
BC142681 mRNA Translation: AAI42682.1
Z16411 mRNA Translation: CAA78903.1
CCDSiCCDS53654.1 [Q01970-2]
CCDS8064.1 [Q01970-1]
PIRiI38994
S27002
S52099
RefSeqiNP_000923.1, NM_000932.2 [Q01970-1]
NP_001171812.1, NM_001184883.1 [Q01970-2]
NP_001303243.1, NM_001316314.1 [Q01970-1]
UniGeneiHs.523761

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OHMX-ray2.70B10-886[»]
4GNKX-ray4.00B/D10-1234[»]
E934-1192[»]
4QJ3X-ray3.00B10-891[»]
4QJ4X-ray3.30B10-891[»]
4QJ5X-ray3.41B10-891[»]
ProteinModelPortaliQ01970
SMRiQ01970
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111347, 19 interactors
CORUMiQ01970
DIPiDIP-41928N
IntActiQ01970, 6 interactors
MINTiQ01970
STRINGi9606.ENSP00000279230

Chemistry databases

BindingDBiQ01970
ChEMBLiCHEMBL5449
SwissLipidsiSLP:000001756

PTM databases

iPTMnetiQ01970
PhosphoSitePlusiQ01970

Polymorphism and mutation databases

BioMutaiPLCB3
DMDMi1730573

Proteomic databases

EPDiQ01970
MaxQBiQ01970
PaxDbiQ01970
PeptideAtlasiQ01970
PRIDEiQ01970
ProteomicsDBi58024

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000279230; ENSP00000279230; ENSG00000149782 [Q01970-1]
ENST00000325234; ENSP00000324660; ENSG00000149782 [Q01970-2]
ENST00000540288; ENSP00000443631; ENSG00000149782 [Q01970-1]
GeneIDi5331
KEGGihsa:5331
UCSCiuc009ypg.3 human [Q01970-1]

Organism-specific databases

CTDi5331
DisGeNETi5331
EuPathDBiHostDB:ENSG00000149782.11
GeneCardsiPLCB3
HGNCiHGNC:9056 PLCB3
HPAiCAB009257
HPA039279
HPA043907
MIMi600230 gene
neXtProtiNX_Q01970
OpenTargetsiENSG00000149782
PharmGKBiPA33386
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiQ01970
KOiK05858
OMAiPHHHGCS
OrthoDBiEOG091G00XL
PhylomeDBiQ01970
TreeFamiTF313216

Enzyme and pathway databases

BRENDAi3.1.4.11 2681
ReactomeiR-HSA-112043 PLC beta mediated events
R-HSA-1855204 Synthesis of IP3 and IP4 in the cytosol
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-4086398 Ca2+ pathway
R-HSA-416476 G alpha (q) signalling events
R-HSA-418217 G beta:gamma signalling through PLC beta
R-HSA-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-HSA-500657 Presynaptic function of Kainate receptors
SIGNORiQ01970

Miscellaneous databases

ChiTaRSiPLCB3 human
GeneWikiiPLCB3
GenomeRNAii5331
PROiPR:Q01970
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000149782 Expressed in 180 organ(s), highest expression level in small intestine Peyer's patch
CleanExiHS_PLCB3
GenevisibleiQ01970 HS

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB3_HUMAN
AccessioniPrimary (citable) accession number: Q01970
Secondary accession number(s): A5PKZ6, G5E960, Q8N1A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 190 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again