UniProtKB - Q01939 (PRS8_YEAST)
Protein
26S proteasome regulatory subunit 8 homolog
Gene
RPT6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity1 Publication
Miscellaneous
Present with 4700 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 189 – 196 | ATP | 8 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- proteasome-activating ATPase activity Source: InterPro
- protein domain specific binding Source: SGD
- TBP-class protein binding Source: GO_Central
- ubiquitin protein ligase binding Source: SGD
GO - Biological processi
- chromatin remodeling Source: SGD
- negative regulation of DNA-binding transcription factor activity Source: SGD
- nonfunctional rRNA decay Source: SGD
- nucleotide-excision repair Source: SGD
- positive regulation of DNA-binding transcription factor activity Source: SGD
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: SGD
- positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- proteasome regulatory particle assembly Source: SGD
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit 8 homologAlternative name(s): Protein CIM3 Protein SUG1 Tat-binding protein TBY1 |
Gene namesi | Name:RPT6 Synonyms:CIM3, CRL3, SUG1, TBPY, TBY1 Ordered Locus Names:YGL048C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003016, RPT6 |
VEuPathDBi | FungiDB:YGL048C |
Subcellular locationi
Cytosol
- cytosolic proteasome complex Source: GO_Central
- proteasome storage granule Source: SGD
Nucleus
- nuclear proteasome complex Source: GO_Central
- nucleus Source: SGD
Other locations
- proteasome regulatory particle, base subcomplex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000084730 | 2 – 405 | 26S proteasome regulatory subunit 8 homologAdd BLAST | 404 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonine1 Publication | 1 |
Post-translational modificationi
N-acetylated by NAT1.1 Publication
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q01939 |
PaxDbi | Q01939 |
PRIDEi | Q01939 |
PTM databases
CarbonylDBi | Q01939 |
iPTMneti | Q01939 |
Interactioni
Subunit structurei
May form a homodimer or a heterodimer with a related family member.
Interacts with OLA1, TMA17, and UBR1.
3 PublicationsBinary interactionsi
Hide detailsQ01939
GO - Molecular functioni
- protein domain specific binding Source: SGD
- TBP-class protein binding Source: GO_Central
- ubiquitin protein ligase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33200, 725 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-979N |
IntActi | Q01939, 84 interactors |
MINTi | Q01939 |
STRINGi | 4932.YGL048C |
Miscellaneous databases
RNActi | Q01939, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Phylogenomic databases
eggNOGi | KOG0728, Eukaryota |
GeneTreei | ENSGT01000000214468 |
HOGENOMi | CLU_000688_2_0_1 |
InParanoidi | Q01939 |
OMAi | MALDTFF |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB IPR035261, PSMC5 |
PANTHERi | PTHR23073:SF102, PTHR23073:SF102, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q01939-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTAAVTSSNI VLETHESGIK PYFEQKIQET ELKIRSKTEN VRRLEAQRNA
60 70 80 90 100
LNDKVRFIKD ELRLLQEPGS YVGEVIKIVS DKKVLVKVQP EGKYIVDVAK
110 120 130 140 150
DINVKDLKAS QRVCLRSDSY MLHKVLENKA DPLVSLMMVE KVPDSTYDMV
160 170 180 190 200
GGLTKQIKEI KEVIELPVKH PELFESLGIA QPKGVILYGP PGTGKTLLAR
210 220 230 240 250
AVAHHTDCKF IRVSGAELVQ KYIGEGSRMV RELFVMAREH APSIIFMDEI
260 270 280 290 300
DSIGSTRVEG SGGGDSEVQR TMLELLNQLD GFETSKNIKI IMATNRLDIL
310 320 330 340 350
DPALLRPGRI DRKIEFPPPS VAARAEILRI HSRKMNLTRG INLRKVAEKM
360 370 380 390 400
NGCSGADVKG VCTEAGMYAL RERRIHVTQE DFELAVGKVM NKNQETAISV
AKLFK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | V → G in CAA47023 (PubMed:1614516).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66400 Genomic DNA Translation: CAA47023.1 L01626 Genomic DNA Translation: AAA35138.1 Z72570 Genomic DNA Translation: CAA96750.1 AY693135 Genomic DNA Translation: AAT93154.1 BK006941 Genomic DNA Translation: DAA08053.1 |
PIRi | S64052 |
RefSeqi | NP_011467.1, NM_001180913.1 |
Genome annotation databases
EnsemblFungii | YGL048C_mRNA; YGL048C; YGL048C |
GeneIDi | 852834 |
KEGGi | sce:YGL048C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66400 Genomic DNA Translation: CAA47023.1 L01626 Genomic DNA Translation: AAA35138.1 Z72570 Genomic DNA Translation: CAA96750.1 AY693135 Genomic DNA Translation: AAT93154.1 BK006941 Genomic DNA Translation: DAA08053.1 |
PIRi | S64052 |
RefSeqi | NP_011467.1, NM_001180913.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCO | electron microscopy | 4.80 | J | 1-405 | [»] | |
3JCP | electron microscopy | 4.60 | J | 1-405 | [»] | |
4CR2 | electron microscopy | 7.70 | J | 1-405 | [»] | |
4CR3 | electron microscopy | 9.30 | J | 1-405 | [»] | |
4CR4 | electron microscopy | 8.80 | J | 1-405 | [»] | |
5A5B | electron microscopy | 9.50 | J | 1-405 | [»] | |
5MP9 | electron microscopy | 4.10 | J | 1-405 | [»] | |
5MPA | electron microscopy | 4.50 | J | 1-405 | [»] | |
5MPB | electron microscopy | 7.80 | J | 1-405 | [»] | |
5MPC | electron microscopy | 7.70 | J | 1-405 | [»] | |
5WVI | electron microscopy | 6.30 | J | 1-405 | [»] | |
5WVK | electron microscopy | 4.20 | J | 1-405 | [»] | |
6EF0 | electron microscopy | 4.43 | J | 130-405 | [»] | |
6EF1 | electron microscopy | 4.73 | J | 133-405 | [»] | |
6EF2 | electron microscopy | 4.27 | J | 144-405 | [»] | |
6EF3 | electron microscopy | 4.17 | J | 1-405 | [»] | |
6FVT | electron microscopy | 4.10 | J | 1-405 | [»] | |
6FVU | electron microscopy | 4.50 | J | 1-405 | [»] | |
6FVV | electron microscopy | 5.40 | J | 1-405 | [»] | |
6FVW | electron microscopy | 4.50 | J | 3-405 | [»] | |
6FVX | electron microscopy | 4.90 | J | 1-405 | [»] | |
6FVY | electron microscopy | 6.10 | J | 1-405 | [»] | |
6J2C | electron microscopy | 7.00 | J | 1-405 | [»] | |
6J2N | electron microscopy | 7.50 | J | 1-405 | [»] | |
6J2Q | electron microscopy | 3.80 | J | 1-405 | [»] | |
6J2X | electron microscopy | 3.80 | J | 1-405 | [»] | |
6J30 | electron microscopy | 4.50 | J | 1-405 | [»] | |
BMRBi | Q01939 | |||||
SMRi | Q01939 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33200, 725 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-979N |
IntActi | Q01939, 84 interactors |
MINTi | Q01939 |
STRINGi | 4932.YGL048C |
PTM databases
CarbonylDBi | Q01939 |
iPTMneti | Q01939 |
Proteomic databases
MaxQBi | Q01939 |
PaxDbi | Q01939 |
PRIDEi | Q01939 |
Genome annotation databases
EnsemblFungii | YGL048C_mRNA; YGL048C; YGL048C |
GeneIDi | 852834 |
KEGGi | sce:YGL048C |
Organism-specific databases
SGDi | S000003016, RPT6 |
VEuPathDBi | FungiDB:YGL048C |
Phylogenomic databases
eggNOGi | KOG0728, Eukaryota |
GeneTreei | ENSGT01000000214468 |
HOGENOMi | CLU_000688_2_0_1 |
InParanoidi | Q01939 |
OMAi | MALDTFF |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:Q01939 |
RNActi | Q01939, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB IPR035261, PSMC5 |
PANTHERi | PTHR23073:SF102, PTHR23073:SF102, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS8_YEAST | |
Accessioni | Q01939Primary (citable) accession number: Q01939 Secondary accession number(s): D6VU92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 201 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families