UniProtKB - Q01860 (PO5F1_HUMAN)
POU domain, class 5, transcription factor 1
POU5F1
Functioni
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 157 | DNABy similarity | 1 | |
Binding sitei | 164 | DNABy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 230 – 289 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding Source: HGNC-UCL
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor activity Source: HGNC-UCL
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: BHF-UCL
- miRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
- transcription factor binding Source: BHF-UCL
- transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- anatomical structure morphogenesis Source: ProtInc
- blastocyst development Source: BHF-UCL
- BMP signaling pathway involved in heart induction Source: BHF-UCL
- cardiac cell fate determination Source: BHF-UCL
- cell differentiation Source: GO_Central
- cell fate commitment involved in formation of primary germ layer Source: BHF-UCL
- endodermal cell fate specification Source: MGI
- negative regulation of gene silencing by miRNA Source: UniProtKB
- negative regulation of pri-miRNA transcription by RNA polymerase II Source: ARUK-UCL
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of canonical Wnt signaling pathway Source: BHF-UCL
- positive regulation of SMAD protein signal transduction Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of asymmetric cell division Source: BHF-UCL
- regulation of DNA methylation-dependent heterochromatin assembly Source: BHF-UCL
- regulation of gene expression Source: UniProtKB
- regulation of heart induction by regulation of canonical Wnt signaling pathway Source: BHF-UCL
- regulation of transcription, DNA-templated Source: HGNC-UCL
- regulation of transcription by RNA polymerase II Source: BHF-UCL
- response to wounding Source: UniProtKB
- somatic stem cell population maintenance Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q01860 |
Reactomei | R-HSA-2892245, POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation R-HSA-2892247, POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation R-HSA-452723, Transcriptional regulation of pluripotent stem cells |
SignaLinki | Q01860 |
SIGNORi | Q01860 |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 5, transcription factor 1Alternative name(s): Octamer-binding protein 3 Short name: Oct-3 Octamer-binding protein 4 Short name: Oct-4 Octamer-binding transcription factor 3 Short name: OTF-3 |
Gene namesi | Name:POU5F1 Synonyms:OCT3, OCT4, OTF3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9221, POU5F1 |
MIMi | 164177, gene |
neXtProti | NX_Q01860 |
VEuPathDBi | HostDB:ENSG00000204531.15 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nucleoplasm Source: BHF-UCL
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Biotechnological usei
Organism-specific databases
DisGeNETi | 5460 |
OpenTargetsi | ENSG00000204531 |
PharmGKBi | PA33545 |
Miscellaneous databases
Pharosi | Q01860, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3580526 |
DrugBanki | DB00988, Dopamine DB00368, Norepinephrine |
Genetic variation databases
BioMutai | POU5F1 |
DMDMi | 400659 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100747 | 1 – 360 | POU domain, class 5, transcription factor 1Add BLAST | 360 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 111 | Phosphoserine; by MAPK2 Publications | 1 | |
Cross-linki | 123 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 235 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 236 | Phosphoserine1 Publication | 1 | |
Modified residuei | 289 | Phosphoserine1 Publication | 1 | |
Modified residuei | 290 | Phosphoserine1 Publication | 1 | |
Modified residuei | 355 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q01860 |
jPOSTi | Q01860 |
MassIVEi | Q01860 |
MaxQBi | Q01860 |
PaxDbi | Q01860 |
PeptideAtlasi | Q01860 |
PRIDEi | Q01860 |
ProteomicsDBi | 58011 [Q01860-1] 58012 [Q01860-2] |
PTM databases
iPTMneti | Q01860 |
PhosphoSitePlusi | Q01860 |
SwissPalmi | Q01860 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSG00000204531, Expressed in muscle tissue and 109 other tissues |
ExpressionAtlasi | Q01860, baseline and differential |
Genevisiblei | Q01860, HS |
Organism-specific databases
HPAi | ENSG00000204531, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PKM.
Interacts with WWP2.
Interacts with UBE2I and ZSCAN10 (By similarity).
Interacts with PCGF1 (PubMed:26687479).
Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity).
Interacts with ZNF322 (By similarity).
Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity).
Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity).
Interacts with FBXW4 (By similarity).
Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ01860
GO - Molecular functioni
- transcription factor binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111456, 323 interactors |
CORUMi | Q01860 |
DIPi | DIP-33440N |
ELMi | Q01860 |
IntActi | Q01860, 19 interactors |
MINTi | Q01860 |
STRINGi | 9606.ENSP00000259915 |
Chemistry databases
BindingDBi | Q01860 |
Miscellaneous databases
RNActi | Q01860, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q01860 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 138 – 212 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 180 – 186 | DNA bindingBy similarity | 7 | |
Regioni | 193 – 196 | DNA bindingBy similarity | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000155046 |
HOGENOMi | CLU_066243_0_0_1 |
InParanoidi | Q01860 |
OMAi | CPQPYEF |
PhylomeDBi | Q01860 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR015585, POU_dom_5 |
PANTHERi | PTHR11636:SF86, PTHR11636:SF86, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGPGIGPG
60 70 80 90 100
VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG
110 120 130 140 150
VGVESNSDGA SPEPCTVTPG AVKLEKEKLE QNPEESQDIK ALQKELEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLENLFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA
310 320 330 340 350
AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVSV
360
TTLGSPMHSN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2PP83 | A0A1W2PP83_HUMAN | POU domain, class 5, transcription ... | POU5F1 | 123 | Annotation score: | ||
U3KQH6 | U3KQH6_HUMAN | POU domain protein | POU5F1 | 190 | Annotation score: | ||
F2Z381 | F2Z381_HUMAN | POU class 5 homeobox 1 transcript v... | POU5F1 | 164 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 189 | A → G in CAA79974 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 220 | I → T in CAA79974 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 227 | V → L in CAA79974 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 230 | R → G in CAA79975 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 240 | R → Q in CAA79975 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 251 | Q → R in CAA79975 (PubMed:7908264).Curated | 1 | |
Sequence conflicti | 272 | D → DVVR in AAB35990 (PubMed:8567814).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_046203 | 226 | L → F. Corresponds to variant dbSNP:rs1150767Ensembl. | 1 | |
Natural variantiVAR_003774 | 322 | T → A1 Publication | 1 | |
Natural variantiVAR_046204 | 351 | T → I. Corresponds to variant dbSNP:rs1061120Ensembl. | 1 | |
Natural variantiVAR_003775 | 357 | M → L1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002333 | 1 – 135 | MAGHL…QNPEE → MHFYRLFLGATRRFLNPEWK GEIDNWCVYVLTSLLPFKIQ in isoform B. 1 PublicationAdd BLAST | 135 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11898 mRNA Translation: CAA77951.1 Z11899 mRNA Translation: CAA77952.1 GQ472773 Genomic DNA Translation: ACZ95700.1 AB088113 Genomic DNA Translation: BAC54946.1 AB202105 Genomic DNA Translation: BAE78630.1 AB103619 Genomic DNA Translation: BAF31281.1 BA000025 Genomic DNA Translation: BAB63311.1 AL662833 Genomic DNA No translation available. AL662844 Genomic DNA No translation available. AL773544 Genomic DNA No translation available. BX088580 Genomic DNA No translation available. CR759815 Genomic DNA No translation available. CR388229 Genomic DNA No translation available. CR847794 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03367.1 CH471081 Genomic DNA Translation: EAX03368.1 BC117435 mRNA Translation: AAI17436.1 BC117437 mRNA Translation: AAI17438.1 Z21963 mRNA Translation: CAA79974.1 Z21964 mRNA Translation: CAA79975.1 S81255 mRNA Translation: AAB35990.1 |
CCDSi | CCDS34391.1 [Q01860-1] |
PIRi | S25561 S32652 |
RefSeqi | NP_001272916.1, NM_001285987.1 [Q01860-2] NP_002692.2, NM_002701.5 [Q01860-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Oct-4 entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11898 mRNA Translation: CAA77951.1 Z11899 mRNA Translation: CAA77952.1 GQ472773 Genomic DNA Translation: ACZ95700.1 AB088113 Genomic DNA Translation: BAC54946.1 AB202105 Genomic DNA Translation: BAE78630.1 AB103619 Genomic DNA Translation: BAF31281.1 BA000025 Genomic DNA Translation: BAB63311.1 AL662833 Genomic DNA No translation available. AL662844 Genomic DNA No translation available. AL773544 Genomic DNA No translation available. BX088580 Genomic DNA No translation available. CR759815 Genomic DNA No translation available. CR388229 Genomic DNA No translation available. CR847794 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03367.1 CH471081 Genomic DNA Translation: EAX03368.1 BC117435 mRNA Translation: AAI17436.1 BC117437 mRNA Translation: AAI17438.1 Z21963 mRNA Translation: CAA79974.1 Z21964 mRNA Translation: CAA79975.1 S81255 mRNA Translation: AAB35990.1 |
CCDSi | CCDS34391.1 [Q01860-1] |
PIRi | S25561 S32652 |
RefSeqi | NP_001272916.1, NM_001285987.1 [Q01860-2] NP_002692.2, NM_002701.5 [Q01860-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6T90 | electron microscopy | 3.05 | K | 1-360 | [»] | |
6YOV | electron microscopy | 3.42 | K | 1-360 | [»] | |
SMRi | Q01860 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111456, 323 interactors |
CORUMi | Q01860 |
DIPi | DIP-33440N |
ELMi | Q01860 |
IntActi | Q01860, 19 interactors |
MINTi | Q01860 |
STRINGi | 9606.ENSP00000259915 |
Chemistry databases
BindingDBi | Q01860 |
ChEMBLi | CHEMBL3580526 |
DrugBanki | DB00988, Dopamine DB00368, Norepinephrine |
PTM databases
iPTMneti | Q01860 |
PhosphoSitePlusi | Q01860 |
SwissPalmi | Q01860 |
Genetic variation databases
BioMutai | POU5F1 |
DMDMi | 400659 |
Proteomic databases
EPDi | Q01860 |
jPOSTi | Q01860 |
MassIVEi | Q01860 |
MaxQBi | Q01860 |
PaxDbi | Q01860 |
PeptideAtlasi | Q01860 |
PRIDEi | Q01860 |
ProteomicsDBi | 58011 [Q01860-1] 58012 [Q01860-2] |
Protocols and materials databases
Antibodypediai | 26854, 1441 antibodies |
DNASUi | 5460 |
Genome annotation databases
Organism-specific databases
CTDi | 5460 |
DisGeNETi | 5460 |
GeneCardsi | POU5F1 |
HGNCi | HGNC:9221, POU5F1 |
HPAi | ENSG00000204531, Low tissue specificity |
MIMi | 164177, gene |
neXtProti | NX_Q01860 |
OpenTargetsi | ENSG00000204531 |
PharmGKBi | PA33545 |
VEuPathDBi | HostDB:ENSG00000204531.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000155046 |
HOGENOMi | CLU_066243_0_0_1 |
InParanoidi | Q01860 |
OMAi | CPQPYEF |
PhylomeDBi | Q01860 |
TreeFami | TF316413 |
Enzyme and pathway databases
PathwayCommonsi | Q01860 |
Reactomei | R-HSA-2892245, POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation R-HSA-2892247, POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation R-HSA-452723, Transcriptional regulation of pluripotent stem cells |
SignaLinki | Q01860 |
SIGNORi | Q01860 |
Miscellaneous databases
BioGRID-ORCSi | 5460, 97 hits in 870 CRISPR screens |
GenomeRNAii | 5460 |
Pharosi | Q01860, Tbio |
PROi | PR:Q01860 |
RNActi | Q01860, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204531, Expressed in muscle tissue and 109 other tissues |
ExpressionAtlasi | Q01860, baseline and differential |
Genevisiblei | Q01860, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR015585, POU_dom_5 |
PANTHERi | PTHR11636:SF86, PTHR11636:SF86, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PO5F1_HUMAN | |
Accessioni | Q01860Primary (citable) accession number: Q01860 Secondary accession number(s): A6NCS1 Q5STF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 213 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants