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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

TIM44

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 and its co-chaperone (MGE1) to drive protein translocation into the matrix using ATP as an energy source.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi101 – 108ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31297-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Alternative name(s):
Inner membrane import site protein 45
Short name:
ISP451 Publication
Membrane import machinery protein MIM44
Mitochondrial protein import protein 1
Gene namesi
Name:TIM44
Synonyms:ISP45, MIM44, MPI11 Publication
Ordered Locus Names:YIL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001284 TIM44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180R → A or K: Disrupts association with the translocase complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000034319? – 431Mitochondrial import inner membrane translocase subunit TIM44
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ01852
PaxDbiQ01852
PRIDEiQ01852

Interactioni

Subunit structurei

Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. Forms part of the receptor complex that consists of at least 3 different proteins (TIM17, TIM23, TIM44).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34966, 85 interactors
ComplexPortaliCPX-539 Mitochondrial inner membrane pre-sequence translocase complex
DIPiDIP-694N
IntActiQ01852, 21 interactors
MINTiQ01852
STRINGi4932.YIL022W

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ01852
SMRiQ01852
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01852

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 209Interaction with SSC11 PublicationAdd BLAST167

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000000051
HOGENOMiHOG000208172
InParanoidiQ01852
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG092C2GAN

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q01852-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHRSTFIRTS GTSSRTLTAR YRSQYTGLLV ARVLFSTSTT RAQGGNPRSP
60 70 80 90 100
LQIFRDTFKK EWEKSQELQE NIKTLQDASG KLGESEAYKK AREAYLKAQR
110 120 130 140 150
GSTIVGKTLK KTGETMEHIA TKAWESELGK NTRKAAAATA KKLDESFEPV
160 170 180 190 200
RQTKIYKEVS EVIDDGESSR YGGFITKEQR RLKRERDLAS GKRHRAVKSN
210 220 230 240 250
EDAGTAVVAT NIESKESFGK KVEDFKEKTV VGRSIQSLKN KLWDESENPL
260 270 280 290 300
IVVMRKITNK VGGFFAETES SRVYSQFKLM DPTFSNESFT RHLREYIVPE
310 320 330 340 350
ILEAYVKGDV KVLKKWFSEA PFNVYAAQQK IFKEQDVYAD GRILDIRGVE
360 370 380 390 400
IVSAKLLAPQ DIPVLVVGCR AQEINLYRKK KTGEIAAGDE ANILMSSYAM
410 420 430
VFTRDPEQID DDETEGWKIL EFVRGGSRQF T
Length:431
Mass (Da):48,854
Last modified:July 1, 1993 - v1
Checksum:i8E98C738178B1E44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA Translation: CAA86970.1
X67276 Genomic DNA Translation: CAA47693.1
BK006942 Genomic DNA Translation: DAA08523.1
PIRiS25196
RefSeqiNP_012242.1, NM_001179372.1

Genome annotation databases

EnsemblFungiiYIL022W; YIL022W; YIL022W
GeneIDi854790
KEGGisce:YIL022W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA Translation: CAA86970.1
X67276 Genomic DNA Translation: CAA47693.1
BK006942 Genomic DNA Translation: DAA08523.1
PIRiS25196
RefSeqiNP_012242.1, NM_001179372.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FXTX-ray3.20A234-425[»]
3QK9X-ray3.10A/B210-431[»]
ProteinModelPortaliQ01852
SMRiQ01852
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34966, 85 interactors
ComplexPortaliCPX-539 Mitochondrial inner membrane pre-sequence translocase complex
DIPiDIP-694N
IntActiQ01852, 21 interactors
MINTiQ01852
STRINGi4932.YIL022W

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Proteomic databases

MaxQBiQ01852
PaxDbiQ01852
PRIDEiQ01852

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL022W; YIL022W; YIL022W
GeneIDi854790
KEGGisce:YIL022W

Organism-specific databases

SGDiS000001284 TIM44

Phylogenomic databases

GeneTreeiENSGT00390000000051
HOGENOMiHOG000208172
InParanoidiQ01852
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG092C2GAN

Enzyme and pathway databases

BioCyciYEAST:G3O-31297-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ01852
PROiPR:Q01852

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTIM44_YEAST
AccessioniPrimary (citable) accession number: Q01852
Secondary accession number(s): D6VVQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 7, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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Main funding by: National Institutes of Health

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