UniProtKB - Q01827 (VMAT2_RAT)
Protein
Synaptic vesicular amine transporter
Gene
Slc18a2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.
GO - Molecular functioni
- amine transmembrane transporter activity Source: RGD
- drug binding Source: RGD
- enzyme binding Source: RGD
- heat shock protein binding Source: RGD
- monoamine transmembrane transporter activity Source: GO_Central
- serotonin:sodium symporter activity Source: RGD
- transmembrane transporter activity Source: GO_Central
- xenobiotic transmembrane transporter activity Source: InterPro
GO - Biological processi
- aging Source: RGD
- aminergic neurotransmitter loading into synaptic vesicle Source: RGD
- cellular response to ammonium ion Source: RGD
- cellular response to drug Source: RGD
- endocytic recycling Source: RGD
- glucose homeostasis Source: RGD
- insulin secretion Source: RGD
- locomotory behavior Source: RGD
- monoamine transport Source: RGD
- negative regulation of neurotransmitter transport Source: RGD
- neurotransmitter loading into synaptic vesicle Source: RGD
- neurotransmitter transport Source: RGD
- post-embryonic development Source: RGD
- response to amphetamine Source: RGD
- response to cocaine Source: RGD
- response to corticosterone Source: RGD
- response to drug Source: RGD
- response to herbicide Source: RGD
- response to metal ion Source: RGD
- response to starvation Source: RGD
- response to toxic substance Source: RGD
- response to zinc ion Source: RGD
Keywordsi
Biological process | Neurotransmitter transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-181429, Serotonin Neurotransmitter Release Cycle R-RNO-181430, Norepinephrine Neurotransmitter Release Cycle R-RNO-212676, Dopamine Neurotransmitter Release Cycle R-RNO-442660, Na+/Cl- dependent neurotransmitter transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptic vesicular amine transporterAlternative name(s): Monoamine transporter Solute carrier family 18 member 2 Vesicular amine transporter 2 Short name: VAT2 |
Gene namesi | Name:Slc18a2 Synonyms:Svat, Vmat2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3694, Slc18a2 |
Subcellular locationi
Other locations
Other locations
- axon terminus Source: RGD
- cell body Source: RGD
- cell projection Source: RGD
- cytoplasm Source: RGD
- dense core granule Source: RGD
- dopaminergic synapse Source: SynGO
- integral component of synaptic vesicle membrane Source: SynGO
- neuron projection Source: RGD
- neuronal cell body Source: RGD
- postsynapse Source: SynGO
- synaptic vesicle Source: RGD
- synaptic vesicle membrane Source: RGD
- terminal bouton Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 21 – 41 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 42 – 130 | Lumenal, vesicleSequence analysisAdd BLAST | 89 | |
Transmembranei | 131 – 151 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 152 – 160 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 161 – 181 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 182 – 190 | Lumenal, vesicleSequence analysis | 9 | |
Transmembranei | 191 – 211 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 212 – 220 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 221 – 243 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 244 – 249 | Lumenal, vesicleSequence analysis | 6 | |
Transmembranei | 250 – 272 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 273 – 292 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 293 – 312 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 313 – 329 | Lumenal, vesicleSequence analysisAdd BLAST | 17 | |
Transmembranei | 330 – 353 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 354 – 358 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 359 – 379 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 380 – 390 | Lumenal, vesicleSequence analysisAdd BLAST | 11 | |
Transmembranei | 391 – 411 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 412 – 415 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 416 – 436 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 437 – 441 | Lumenal, vesicleSequence analysis | 5 | |
Transmembranei | 442 – 463 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 464 – 515 | CytoplasmicSequence analysisAdd BLAST | 52 |
Keywords - Cellular componenti
Cytoplasmic vesicle, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL4828 |
DrugCentrali | Q01827 |
GuidetoPHARMACOLOGYi | 1012 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127516 | 1 – 515 | Synaptic vesicular amine transporterAdd BLAST | 515 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 56 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 81 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 111 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 118 ↔ 325 | By similarity | ||
Modified residuei | 512 | Phosphoserine; by CK2Combined sources1 Publication | 1 | |
Modified residuei | 514 | Phosphoserine; by CK2Combined sources1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q01827 |
PRIDEi | Q01827 |
PTM databases
GlyGeni | Q01827, 5 sites |
iPTMneti | Q01827 |
PhosphoSitePlusi | Q01827 |
Expressioni
Tissue specificityi
Brainstem and stomach.
Interactioni
Subunit structurei
Interacts with SLC6A3.
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- heat shock protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247580, 2 interactors |
CORUMi | Q01827 |
STRINGi | 10116.ENSRNOP00000011983 |
Chemistry databases
BindingDBi | Q01827 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3764, Eukaryota |
InParanoidi | Q01827 |
OrthoDBi | 956763at2759 |
PhylomeDBi | Q01827 |
TreeFami | TF313494 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q01827-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNSTEI QTTRPELVVS TSESIFSYYN NSTVLITGNA TGTLPGGQSH
110 120 130 140 150
KATSTQHTVA NTTVPSDCPS EDRDLLNENV QVGLLFASKA TVQLLTNPFI
160 170 180 190 200
GLLTNRIGYP IPMFAGFCIM FISTVMFAFS SSYAFLLIAR SLQGIGSSCS
210 220 230 240 250
SVAGMGMLAS VYTDDEERGK PMGIALGGLA MGVLVGPPFG SVLYEFVGKT
260 270 280 290 300
APFLVLAALV LLDGAIQLFV LQPSRVQPES QKGTPLTTLL KDPYILIAAG
310 320 330 340 350
SICFANMGIA MLEPALPIWM METMCSRKWQ LGVAFLPASI SYLIGTNIFG
360 370 380 390 400
ILAHKMGRWL CALLGMVIVG ISILCIPFAK NIYGLIAPNF GVGFAIGMVD
410 420 430 440 450
SSMMPIMGYL VDLRHVSVYG SVYAIADVAF CMGYAIGPSA GGAIAKAIGF
460 470 480 490 500
PWLMTIIGII DIAFAPLCFF LRSPPAKEEK MAILMDHNCP IKRKMYTQNN
510
VQSYPIGDDE ESESD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JSJ6 | A0A0G2JSJ6_RAT | Solute carrier family 18 (Vesicular... | Slc18a2 rCG_57498 | 515 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 – 221 | KP → NA in AAA41627 (PubMed:1438304).Curated | 2 | |
Sequence conflicti | 397 | G → F in AAA42190 (PubMed:1505023).Curated | 1 | |
Sequence conflicti | 493 | R → T in AAA41627 (PubMed:1438304).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97381 mRNA Translation: AAA42190.1 L00603 mRNA Translation: AAA41627.1 |
PIRi | A46374 |
RefSeqi | NP_037163.1, NM_013031.1 XP_006231726.1, XM_006231664.3 XP_017444313.1, XM_017588824.1 |
Genome annotation databases
GeneIDi | 25549 |
KEGGi | rno:25549 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97381 mRNA Translation: AAA42190.1 L00603 mRNA Translation: AAA41627.1 |
PIRi | A46374 |
RefSeqi | NP_037163.1, NM_013031.1 XP_006231726.1, XM_006231664.3 XP_017444313.1, XM_017588824.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 247580, 2 interactors |
CORUMi | Q01827 |
STRINGi | 10116.ENSRNOP00000011983 |
Chemistry databases
BindingDBi | Q01827 |
ChEMBLi | CHEMBL4828 |
DrugCentrali | Q01827 |
GuidetoPHARMACOLOGYi | 1012 |
PTM databases
GlyGeni | Q01827, 5 sites |
iPTMneti | Q01827 |
PhosphoSitePlusi | Q01827 |
Proteomic databases
PaxDbi | Q01827 |
PRIDEi | Q01827 |
Protocols and materials databases
ABCDi | Q01827, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 25549 |
KEGGi | rno:25549 |
Organism-specific databases
CTDi | 6571 |
RGDi | 3694, Slc18a2 |
Phylogenomic databases
eggNOGi | KOG3764, Eukaryota |
InParanoidi | Q01827 |
OrthoDBi | 956763at2759 |
PhylomeDBi | Q01827 |
TreeFami | TF313494 |
Enzyme and pathway databases
Reactomei | R-RNO-181429, Serotonin Neurotransmitter Release Cycle R-RNO-181430, Norepinephrine Neurotransmitter Release Cycle R-RNO-212676, Dopamine Neurotransmitter Release Cycle R-RNO-442660, Na+/Cl- dependent neurotransmitter transporters |
Miscellaneous databases
PROi | PR:Q01827 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VMAT2_RAT | |
Accessioni | Q01827Primary (citable) accession number: Q01827 Secondary accession number(s): Q9QVP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | September 19, 2003 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families