UniProtKB - Q01786 (GUN5_THEFU)

BLAST

>sp|Q01786|GUN5_THEFU Endoglucanase E-5 OS=Thermobifida fusca OX=2021 GN=celE PE=1 SV=2
MAKSPAARKGXPPVAVAVTAALALLIALLSPGVAQAAGLTATVTKESSWDNGYSASVTVR
NDTSSTVSQWEVVLTLPGGTTVAQVWNAQHTSSGNSHTFTGVSWNSTIPPGGTASSGFIA
SGSGEPTHCTINGAPCDEGSEPGGPGGPGTPSPDPGTQPGTGTPVERYGKVQVCGTQLCD
EHGNPVQLRGMSTHGIQWFDHCLTDSSLDALAYDWKADIIRLSMYIQEDGYETNPRGFTD
RMHQLIDMATARGLYVIVDWHILTPGDPHYNLDRAKTFFAEIAQRHASKTNVLYEIANEP
NGVSWASIKSYAEEVIPVIRQRDPDSVIIVGTRGWSSLGVSEGSGPAEIAANPVNASNIM
YAFHFYAASHRDNYLNALREASELFPVFVTEFGTETYTGDGANDFQMADRYIDLMAERKI
GWTKWNYSDDFRSGAVFQPGTCASGGPWSGSSLKASGQWVRSKLQS

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History
Entry version 105 (23 May 2018)
Sequence version 2 (15 Jul 1999)
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Protein

Endoglucanase E-5

Gene

celE

Organism

Thermobifida fusca (Thermomonospora fusca)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalytic activityⁱ

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayⁱ: cellulose degradation

This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	299	Proton donorBy similarity		1
Active siteⁱ	391	NucleophileBy similarity		1

GO - Molecular functionⁱ

cellulase activity Source: UniProtKB-EC
polysaccharide binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellulose catabolic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Biological process	Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayⁱ	UPA00696

UniPathwayⁱ

UPA00696

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM2 Carbohydrate-Binding Module Family 2 GH5 Glycoside Hydrolase Family 5

CAZyⁱ

CBM2 Carbohydrate-Binding Module Family 2
GH5 Glycoside Hydrolase Family 5

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Endoglucanase E-5 (EC:3.2.1.4) Alternative name(s): Cellulase E-5 Cellulase E5 Endo-1,4-beta-glucanase E-4
Gene namesⁱ	Name:celE
Organismⁱ	Thermobifida fusca (Thermomonospora fusca)
Taxonomic identifierⁱ	2021 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Actinobacteria › Actinobacteria › Streptosporangiales › Nocardiopsaceae › Thermobifida

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 36	Add BLAST		36
ChainⁱPRO_0000007866	37 – 466	Endoglucanase E-5Add BLAST		430

Proteomic databases

PRIDEⁱ	Q01786

PRIDEⁱ

Q01786

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	164 – 168	Combined sources	5
Beta strandⁱ	172 – 174	Combined sources	3
Beta strandⁱ	177 – 179	Combined sources	3
Beta strandⁱ	189 – 192	Combined sources	4
Helixⁱ	196 – 199	Combined sources	4
Helixⁱ	200 – 202	Combined sources	3
Helixⁱ	205 – 213	Combined sources	9
Beta strandⁱ	218 – 227	Combined sources	10
Helixⁱ	231 – 233	Combined sources	3
Helixⁱ	235 – 250	Combined sources	16
Turnⁱ	251 – 253	Combined sources	3
Beta strandⁱ	255 – 261	Combined sources	7
Helixⁱ	268 – 271	Combined sources	4
Helixⁱ	272 – 286	Combined sources	15
Beta strandⁱ	290 – 295	Combined sources	6
Helixⁱ	305 – 322	Combined sources	18
Beta strandⁱ	328 – 330	Combined sources	3
Helixⁱ	333 – 336	Combined sources	4
Helixⁱ	340 – 342	Combined sources	3
Helixⁱ	347 – 351	Combined sources	5
Beta strandⁱ	357 – 366	Combined sources	10
Turnⁱ	367 – 369	Combined sources	3
Helixⁱ	372 – 384	Combined sources	13
Beta strandⁱ	387 – 395	Combined sources	9
Helixⁱ	405 – 418	Combined sources	14
Beta strandⁱ	422 – 425	Combined sources	4
Beta strandⁱ	435 – 437	Combined sources	3
Helixⁱ	441 – 444	Combined sources	4
Helixⁱ	450 – 452	Combined sources	3
Helixⁱ	455 – 465	Combined sources	11

Show more details

3D structure databases

ProteinModelPortalⁱ	Q01786
SMRⁱ	Q01786
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q01786

EvolutionaryTraceⁱ

Q01786

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	37 – 139	CBM2PROSITE-ProRule annotationAdd BLAST		103

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG4107SMB Bacteria COG2730 LUCA

eggNOGⁱ

ENOG4107SMB Bacteria
COG2730 LUCA

Family and domain databases

Gene3Dⁱ	2.60.40.290, 1 hit
InterProⁱ	View protein in InterPro IPR001919 CBD2 IPR008965 CBM2/CBM3_carb-bd_dom_sf IPR012291 CBM2_carb-bd_dom_sf IPR001547 Glyco_hydro_5 IPR018087 Glyco_hydro_5_CS IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00553 CBM_2, 1 hit PF00150 Cellulase, 1 hit
SMARTⁱ	View protein in SMART SM00637 CBD_II, 1 hit
SUPFAMⁱ	SSF49384 SSF49384, 1 hit SSF51445 SSF51445, 1 hit
PROSITEⁱ	View protein in PROSITE PS51173 CBM2, 1 hit PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q01786-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAKSPAARKG XPPVAVAVTA ALALLIALLS PGVAQAAGLT ATVTKESSWD 
        60         70         80         90        100
NGYSASVTVR NDTSSTVSQW EVVLTLPGGT TVAQVWNAQH TSSGNSHTFT 
       110        120        130        140        150
GVSWNSTIPP GGTASSGFIA SGSGEPTHCT INGAPCDEGS EPGGPGGPGT 
       160        170        180        190        200
PSPDPGTQPG TGTPVERYGK VQVCGTQLCD EHGNPVQLRG MSTHGIQWFD 
       210        220        230        240        250
HCLTDSSLDA LAYDWKADII RLSMYIQEDG YETNPRGFTD RMHQLIDMAT 
       260        270        280        290        300
ARGLYVIVDW HILTPGDPHY NLDRAKTFFA EIAQRHASKT NVLYEIANEP 
       310        320        330        340        350
NGVSWASIKS YAEEVIPVIR QRDPDSVIIV GTRGWSSLGV SEGSGPAEIA 
       360        370        380        390        400
ANPVNASNIM YAFHFYAASH RDNYLNALRE ASELFPVFVT EFGTETYTGD 
       410        420        430        440        450
GANDFQMADR YIDLMAERKI GWTKWNYSDD FRSGAVFQPG TCASGGPWSG 
       460 
SSLKASGQWV RSKLQS

Length:466

Mass (Da):49,801

Last modified:July 15, 1999 - v2

Checksum:ⁱ1CF0ADFBF2DEF82E

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L01577 Genomic DNA Translation: AAC09379.1
PIRⁱ	C42360

Protein	Similar proteins		Species	Score	Length	Source
Q01786	Endoglucanase		THEFY		466	UniRef90_Q01786
UPI0001529951		THEFU		306
UPI000D1A9EB5		THEFU		312

Protein	Similar proteins		Species	Score	Length	Source
Q01786	Endoglucanase CelA		STRLI		459	UniRef50_P27035
Endoglucanase		STRCO		486
Endoglucanase		Streptomyces sp. 111WW2		461
Endoglucanase		Streptomyces lividans TK24		461
Endoglucanase		Streptomyces sp. M1013		461
+144

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L01577 Genomic DNA Translation: AAC09379.1
PIRⁱ	C42360

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2CKR	X-ray	1.77	A/B	161-466	[»]
	2CKS	X-ray	1.60	A/B	161-466	[»]
ProteinModelPortalⁱ	Q01786
SMRⁱ	Q01786
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

CAZyⁱ	CBM2 Carbohydrate-Binding Module Family 2 GH5 Glycoside Hydrolase Family 5

CAZyⁱ

CBM2 Carbohydrate-Binding Module Family 2
GH5 Glycoside Hydrolase Family 5

Proteomic databases

PRIDEⁱ	Q01786

PRIDEⁱ

Q01786

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

eggNOGⁱ	ENOG4107SMB Bacteria COG2730 LUCA

eggNOGⁱ

ENOG4107SMB Bacteria
COG2730 LUCA

Enzyme and pathway databases

UniPathwayⁱ	UPA00696

UniPathwayⁱ

UPA00696

Miscellaneous databases

EvolutionaryTraceⁱ	Q01786

EvolutionaryTraceⁱ

Q01786

Family and domain databases

Gene3Dⁱ	2.60.40.290, 1 hit
InterProⁱ	View protein in InterPro IPR001919 CBD2 IPR008965 CBM2/CBM3_carb-bd_dom_sf IPR012291 CBM2_carb-bd_dom_sf IPR001547 Glyco_hydro_5 IPR018087 Glyco_hydro_5_CS IPR017853 Glycoside_hydrolase_SF
Pfamⁱ	View protein in Pfam PF00553 CBM_2, 1 hit PF00150 Cellulase, 1 hit
SMARTⁱ	View protein in SMART SM00637 CBD_II, 1 hit
SUPFAMⁱ	SSF49384 SSF49384, 1 hit SSF51445 SSF51445, 1 hit
PROSITEⁱ	View protein in PROSITE PS51173 CBM2, 1 hit PS00659 GLYCOSYL_HYDROL_F5, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GUN5_THEFU
Accessionⁱ	Q01786Primary (citable) accession number: Q01786
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	July 15, 1999
	Last modified:	May 23, 2018
	This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Direct protein sequencing

Documents

Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q01786 (GUN5_THEFU)

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Endoglucanase E-5

celE

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: cellulose degradation

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

Pathwayⁱ: cellulose degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ