DR1

Functionⁱ

The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.2 Publications

GO - Molecular functionⁱ

DNA binding
Source: ProtInc
Traceable author statementⁱ
- 8670811
protein heterodimerization activity Source: InterPro
RNA polymerase II transcription factor activity, sequence-specific DNA binding
Source: NTNU_SB
Inferred from sequence modelⁱ
- 19274049
TBP-class protein binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 18838386
transcription corepressor activity
Source: ProtInc
Traceable author statementⁱ
- 8670811
transcription factor binding
Source: ProtInc
Traceable author statementⁱ
- 1339312

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

histone H3 acetylation
Source: BHF-UCL
Inferred from direct assayⁱ
- 18838386
negative regulation of transcription by RNA polymerase II
Source: ProtInc
Traceable author statementⁱ
- 8670811
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-3214847 HATs acetylate histones
SIGNORⁱ	Q01658

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein Dr1 Alternative name(s): Down-regulator of transcription 1 Negative cofactor 2-beta Short name: NC2-beta TATA-binding protein-associated phosphoprotein
Gene namesⁱ	Name:DR1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000117505.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3017 DR1
MIMⁱ	601482 gene
neXtProtⁱ	NX_Q01658

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	1810
Open Targets More...OpenTargetsⁱ	ENSG00000117505
PharmGKBⁱ	PA27475

Polymorphism and mutation databases

BioMutaⁱ	DR1
DMDMⁱ	401162

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000072440	2 – 176	Protein Dr1Add BLAST		175

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineCombined sources	1
Modified residueⁱ	105	PhosphoserineCombined sources	1
Modified residueⁱ	106	PhosphoserineCombined sources	1
Modified residueⁱ	166	PhosphoserineCombined sources	1
Modified residueⁱ	167	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q01658
PaxDbⁱ	Q01658
PeptideAtlas More...PeptideAtlasⁱ	Q01658
PRIDEⁱ	Q01658
ProteomicsDBⁱ	57979

PTM databases

iPTMnetⁱ	Q01658
PhosphoSitePlusⁱ	Q01658

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000117505 Expressed in 246 organ(s), highest expression level in choroid plexus epithelium
CleanExⁱ	HS_DR1
ExpressionAtlasⁱ	Q01658 baseline and differential
Genevisibleⁱ	Q01658 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA050785 HPA055308

HPAⁱ

HPA050785
HPA055308

Interactionⁱ

Subunit structureⁱ

Heterodimer with DRAP1. DR1 exists in solution as a homotetramer that dissociates during interaction with TBP and then, after complexing with TBP, reassociates at a slow rate, to reconstitute the tetramer. Interacts with NFIL3. Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
POLE3	Q9NRF9	3	EBI-750300,EBI-744901

With

Entry

#Exp.

IntAct

Notes

POLE3

Q9NRF9

3

EBI-750300,EBI-744901

GO - Molecular functionⁱ

protein heterodimerization activity Source: InterPro
TBP-class protein binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 18838386
transcription factor binding
Source: ProtInc
Traceable author statementⁱ
- 1339312

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	108144, 47 interactors
ComplexPortalⁱ	CPX-1004 PCAF-containing ATAC complex CPX-997 GCN5-containing ATAC complex
CORUMⁱ	Q01658
IntActⁱ	Q01658, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q01658
STRINGⁱ	9606.ENSP00000359290

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	15 – 25	Combined sources	11
Helixⁱ	33 – 60	Combined sources	28
Beta strandⁱ	64 – 66	Combined sources	3
Helixⁱ	68 – 78	Combined sources	11
Helixⁱ	81 – 83	Combined sources	3
Helixⁱ	84 – 110	Combined sources	27
Beta strandⁱ	111 – 113	Combined sources	3
Helixⁱ	115 – 140	Combined sources	26

Show more details

3D structure databases

ProteinModelPortalⁱ	Q01658
SMRⁱ	Q01658
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q01658

EvolutionaryTraceⁱ

Q01658

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	12 – 75	Histone-foldSequence analysisAdd BLAST		64

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	100 – 103	Nuclear localization signalSequence analysis		4

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	121 – 168	Ala/Gln-richAdd BLAST		48

Sequence similaritiesⁱ

Belongs to the NC2 beta/DR1 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0871 Eukaryota COG5150 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000075010
HOGENOMⁱ	HOG000178641
HOVERGENⁱ	HBG002051
InParanoidⁱ	Q01658
KEGG Orthology (KO) More...KOⁱ	K21751
OMAⁱ	TIACDHI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ZDR
PhylomeDBⁱ	Q01658
TreeFamⁱ	TF317588

Family and domain databases

Gene3Dⁱ	1.10.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR003958 CBFA_NFYB_domain IPR009072 Histone-fold
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMⁱ	SSF47113 SSF47113, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q01658-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI 
        60         70         80         90        100
SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK 
       110        120        130        140        150
RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ 
       160        170 
AQLAAASASA SNQAGSSQDE EDDDDI

Length:176

Mass (Da):19,444

Last modified:July 1, 1993 - v1

Checksum:ⁱ36E7E59F2FD6CAB5

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_034506	171	E → D. Corresponds to variant dbSNP:rs3088371Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M97388 mRNA Translation: AAA58442.1 BT006972 mRNA Translation: AAP35618.1 AL137159 Genomic DNA No translation available. BC002809 mRNA Translation: AAH02809.1 BC035507 mRNA Translation: AAH35507.1 BC068553 mRNA Translation: AAH68553.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS744.1
PIRⁱ	A43320
NCBI Reference Sequences More...RefSeqⁱ	NP_001929.1, NM_001938.2
UniGeneⁱ	Hs.348418

Genome annotation databases

Ensemblⁱ	ENST00000370267; ENSP00000359290; ENSG00000117505 ENST00000370272; ENSP00000359295; ENSG00000117505
GeneIDⁱ	1810
KEGGⁱ	hsa:1810

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q01658	Down-regulator of transcription 1, TBP-binding (Negative cofactor 2), isoform CRA_a		HUMAN		176	UniRef100_Q01658
DR1 isoform 1		PANTR		176
Protein Dr1		MACMU		176
Protein Dr1		CALJA		176
Down-regulator of transcription 1, TBP-binding (Negative cofactor 2)		BOVIN		176
+24

Protein	Similar proteins		Species	Score	Length	Source
Q01658	Protein Dr1		RAT		176	UniRef90_Q01658
Protein Dr1		CHICK		176
Protein Dr1		MOUSE		176
Down-regulator of transcription 1, TBP-binding (Negative cofactor 2), isoform CRA_a		HUMAN		176
DR1 isoform 1		PANTR		176
+169

Protein	Similar proteins		Species	Score	Length	Source
Q01658	Protein Dr1		RAT		176	UniRef50_Q01658
Protein Dr1		MOUSE		176
Protein Dr1		CHICK		176
Down-regulator of transcription 1, TBP-binding (Negative cofactor 2), isoform CRA_a		HUMAN		176
DR1 isoform 1		PANTR		176
+175

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M97388 mRNA Translation: AAA58442.1 BT006972 mRNA Translation: AAP35618.1 AL137159 Genomic DNA No translation available. BC002809 mRNA Translation: AAH02809.1 BC035507 mRNA Translation: AAH35507.1 BC068553 mRNA Translation: AAH68553.1
CCDSⁱ	CCDS744.1
PIRⁱ	A43320
RefSeqⁱ	NP_001929.1, NM_001938.2
UniGeneⁱ	Hs.348418

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1JFI	X-ray	2.62	B	1-176	[»]
ProteinModelPortalⁱ	Q01658
SMRⁱ	Q01658
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	108144, 47 interactors
ComplexPortalⁱ	CPX-1004 PCAF-containing ATAC complex CPX-997 GCN5-containing ATAC complex
CORUMⁱ	Q01658
IntActⁱ	Q01658, 10 interactors
MINTⁱ	Q01658
STRINGⁱ	9606.ENSP00000359290

PTM databases

iPTMnetⁱ	Q01658
PhosphoSitePlusⁱ	Q01658

Polymorphism and mutation databases

BioMutaⁱ	DR1
DMDMⁱ	401162

Proteomic databases

EPDⁱ	Q01658
PaxDbⁱ	Q01658
PeptideAtlasⁱ	Q01658
PRIDEⁱ	Q01658
ProteomicsDBⁱ	57979

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	1810
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000370267; ENSP00000359290; ENSG00000117505 ENST00000370272; ENSP00000359295; ENSG00000117505
GeneIDⁱ	1810
KEGGⁱ	hsa:1810

Organism-specific databases

CTDⁱ	1810
DisGeNETⁱ	1810
EuPathDBⁱ	HostDB:ENSG00000117505.12
GeneCardsⁱ	DR1
HGNCⁱ	HGNC:3017 DR1
HPAⁱ	HPA050785 HPA055308
MIMⁱ	601482 gene
neXtProtⁱ	NX_Q01658
OpenTargetsⁱ	ENSG00000117505
PharmGKBⁱ	PA27475
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0871 Eukaryota COG5150 LUCA
GeneTreeⁱ	ENSGT00550000075010
HOGENOMⁱ	HOG000178641
HOVERGENⁱ	HBG002051
InParanoidⁱ	Q01658
KOⁱ	K21751
OMAⁱ	TIACDHI
OrthoDBⁱ	EOG091G0ZDR
PhylomeDBⁱ	Q01658
TreeFamⁱ	TF317588

Enzyme and pathway databases

Reactomeⁱ	R-HSA-3214847 HATs acetylate histones
SIGNORⁱ	Q01658

Miscellaneous databases

ChiTaRSⁱ	DR1 human
EvolutionaryTraceⁱ	Q01658
GeneWikiⁱ	DR1_(gene)
GenomeRNAiⁱ	1810
Protein Ontology More...PROⁱ	PR:Q01658
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000117505 Expressed in 246 organ(s), highest expression level in choroid plexus epithelium
CleanExⁱ	HS_DR1
ExpressionAtlasⁱ	Q01658 baseline and differential
Genevisibleⁱ	Q01658 HS

Family and domain databases

Gene3Dⁱ	1.10.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR003958 CBFA_NFYB_domain IPR009072 Histone-fold
Pfamⁱ	View protein in Pfam PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMⁱ	SSF47113 SSF47113, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NC2B_HUMAN
Accessionⁱ	Q01658Primary (citable) accession number: Q01658
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	July 1, 1993
	Last modified:	September 12, 2018
	This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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Supporting data

UniProtKB - Q01658 (NC2B_HUMAN)

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Protein Dr1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ