UniProtKB - Q01658 (NC2B_HUMAN)
Protein
Protein Dr1
Gene
DR1
Organism
Homo sapiens (Human)
Status
Functioni
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.2 Publications
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: GO_Central
- protein heterodimerization activity Source: InterPro
- RNA polymerase II general transcription initiation factor activity Source: ARUK-UCL
- TBP-class protein binding Source: BHF-UCL
- transcription corepressor activity Source: GO_Central
GO - Biological processi
- chromatin remodeling Source: GO_Central
- histone H3 acetylation Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- RNA polymerase II preinitiation complex assembly Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q01658 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
SIGNORi | Q01658 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Dr1Alternative name(s): Down-regulator of transcription 1 Negative cofactor 2-beta Short name: NC2-beta TATA-binding protein-associated phosphoprotein |
Gene namesi | Name:DR1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000117505.12 |
HGNCi | HGNC:3017, DR1 |
MIMi | 601482, gene |
neXtProti | NX_Q01658 |
Subcellular locationi
Nucleus
Nucleus
- Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
- nucleoplasm Source: HPA
Other locations
- negative cofactor 2 complex Source: GO_Central
- RNA polymerase II transcription regulator complex Source: ARUK-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 1810 |
OpenTargetsi | ENSG00000117505 |
PharmGKBi | PA27475 |
Miscellaneous databases
Pharosi | Q01658, Tbio |
Polymorphism and mutation databases
BioMutai | DR1 |
DMDMi | 401162 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000072440 | 2 – 176 | Protein Dr1Add BLAST | 175 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 105 | PhosphoserineCombined sources | 1 | |
Modified residuei | 106 | PhosphoserineCombined sources | 1 | |
Modified residuei | 166 | PhosphoserineCombined sources | 1 | |
Modified residuei | 167 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q01658 |
jPOSTi | Q01658 |
MassIVEi | Q01658 |
PaxDbi | Q01658 |
PeptideAtlasi | Q01658 |
PRIDEi | Q01658 |
ProteomicsDBi | 57979 |
PTM databases
iPTMneti | Q01658 |
PhosphoSitePlusi | Q01658 |
Expressioni
Gene expression databases
Bgeei | ENSG00000117505, Expressed in choroid plexus epithelium and 257 other tissues |
ExpressionAtlasi | Q01658, baseline and differential |
Genevisiblei | Q01658, HS |
Organism-specific databases
HPAi | ENSG00000117505, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer with DRAP1. DR1 exists in solution as a homotetramer that dissociates during interaction with TBP and then, after complexing with TBP, reassociates at a slow rate, to reconstitute the tetramer.
Interacts with NFIL3.
Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1.
4 PublicationsBinary interactionsi
Hide detailsQ01658
With | #Exp. | IntAct |
---|---|---|
DRAP1 [Q14919] | 31 | EBI-750300,EBI-712941 |
NFYC - isoform 1 [Q13952-2] | 3 | EBI-750300,EBI-11956831 |
POLE3 [Q9NRF9] | 7 | EBI-750300,EBI-744901 |
TBP [Q32MN6] | 3 | EBI-750300,EBI-10239991 |
GO - Molecular functioni
- protein heterodimerization activity Source: InterPro
- TBP-class protein binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 108144, 49 interactors |
ComplexPortali | CPX-1004, PCAF-containing ATAC complex CPX-997, GCN5-containing ATAC complex |
CORUMi | Q01658 |
IntActi | Q01658, 14 interactors |
MINTi | Q01658 |
STRINGi | 9606.ENSP00000359295 |
Miscellaneous databases
RNActi | Q01658, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q01658 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q01658 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 75 | Histone-foldSequence analysisAdd BLAST | 64 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 100 – 103 | Nuclear localization signalSequence analysis | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 121 – 168 | Ala/Gln-richAdd BLAST | 48 |
Sequence similaritiesi
Belongs to the NC2 beta/DR1 family.Curated
Phylogenomic databases
eggNOGi | KOG0871, Eukaryota |
GeneTreei | ENSGT00550000075010 |
HOGENOMi | CLU_066247_11_1_1 |
InParanoidi | Q01658 |
OMAi | ANSGMSM |
OrthoDBi | 1465912at2759 |
PhylomeDBi | Q01658 |
TreeFami | TF317588 |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID00206 |
InterProi | View protein in InterPro IPR003958, CBFA_NFYB_domain IPR009072, Histone-fold IPR042225, Ncb2 |
PANTHERi | PTHR46138, PTHR46138, 1 hit |
Pfami | View protein in Pfam PF00808, CBFD_NFYB_HMF, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q01658-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI
60 70 80 90 100
SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK
110 120 130 140 150
RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ
160 170
AQLAAASASA SNQAGSSQDE EDDDDI
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_034506 | 171 | E → D. Corresponds to variant dbSNP:rs3088371Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97388 mRNA Translation: AAA58442.1 BT006972 mRNA Translation: AAP35618.1 AL137159 Genomic DNA No translation available. BC002809 mRNA Translation: AAH02809.1 BC035507 mRNA Translation: AAH35507.1 BC068553 mRNA Translation: AAH68553.1 |
CCDSi | CCDS744.1 |
PIRi | A43320 |
RefSeqi | NP_001929.1, NM_001938.2 |
Genome annotation databases
Ensembli | ENST00000370267; ENSP00000359290; ENSG00000117505 ENST00000370272; ENSP00000359295; ENSG00000117505 |
GeneIDi | 1810 |
KEGGi | hsa:1810 |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97388 mRNA Translation: AAA58442.1 BT006972 mRNA Translation: AAP35618.1 AL137159 Genomic DNA No translation available. BC002809 mRNA Translation: AAH02809.1 BC035507 mRNA Translation: AAH35507.1 BC068553 mRNA Translation: AAH68553.1 |
CCDSi | CCDS744.1 |
PIRi | A43320 |
RefSeqi | NP_001929.1, NM_001938.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JFI | X-ray | 2.62 | B | 1-176 | [»] | |
SMRi | Q01658 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108144, 49 interactors |
ComplexPortali | CPX-1004, PCAF-containing ATAC complex CPX-997, GCN5-containing ATAC complex |
CORUMi | Q01658 |
IntActi | Q01658, 14 interactors |
MINTi | Q01658 |
STRINGi | 9606.ENSP00000359295 |
PTM databases
iPTMneti | Q01658 |
PhosphoSitePlusi | Q01658 |
Polymorphism and mutation databases
BioMutai | DR1 |
DMDMi | 401162 |
Proteomic databases
EPDi | Q01658 |
jPOSTi | Q01658 |
MassIVEi | Q01658 |
PaxDbi | Q01658 |
PeptideAtlasi | Q01658 |
PRIDEi | Q01658 |
ProteomicsDBi | 57979 |
Protocols and materials databases
Antibodypediai | 19942, 193 antibodies |
DNASUi | 1810 |
Genome annotation databases
Ensembli | ENST00000370267; ENSP00000359290; ENSG00000117505 ENST00000370272; ENSP00000359295; ENSG00000117505 |
GeneIDi | 1810 |
KEGGi | hsa:1810 |
Organism-specific databases
CTDi | 1810 |
DisGeNETi | 1810 |
EuPathDBi | HostDB:ENSG00000117505.12 |
GeneCardsi | DR1 |
HGNCi | HGNC:3017, DR1 |
HPAi | ENSG00000117505, Low tissue specificity |
MIMi | 601482, gene |
neXtProti | NX_Q01658 |
OpenTargetsi | ENSG00000117505 |
PharmGKBi | PA27475 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0871, Eukaryota |
GeneTreei | ENSGT00550000075010 |
HOGENOMi | CLU_066247_11_1_1 |
InParanoidi | Q01658 |
OMAi | ANSGMSM |
OrthoDBi | 1465912at2759 |
PhylomeDBi | Q01658 |
TreeFami | TF317588 |
Enzyme and pathway databases
PathwayCommonsi | Q01658 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
SIGNORi | Q01658 |
Miscellaneous databases
BioGRID-ORCSi | 1810, 537 hits in 861 CRISPR screens |
ChiTaRSi | DR1, human |
EvolutionaryTracei | Q01658 |
GeneWikii | DR1_(gene) |
GenomeRNAii | 1810 |
Pharosi | Q01658, Tbio |
PROi | PR:Q01658 |
RNActi | Q01658, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117505, Expressed in choroid plexus epithelium and 257 other tissues |
ExpressionAtlasi | Q01658, baseline and differential |
Genevisiblei | Q01658, HS |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID00206 |
InterProi | View protein in InterPro IPR003958, CBFA_NFYB_domain IPR009072, Histone-fold IPR042225, Ncb2 |
PANTHERi | PTHR46138, PTHR46138, 1 hit |
Pfami | View protein in Pfam PF00808, CBFD_NFYB_HMF, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NC2B_HUMAN | |
Accessioni | Q01658Primary (citable) accession number: Q01658 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations